Success Rate of MTA Pulpotomy on Vital Pulp of Primary Molars: A 3-Year Observational Study
Brinda Godhi
Citation Information :
Godhi B. Success Rate of MTA Pulpotomy on Vital Pulp of Primary Molars: A 3-Year Observational Study. Int J Clin Pediatr Dent 2016; 9 (3):222-227.
Introduction: Vital pulp therapy is a major contributor in the preservation of primary dentition after caries affliction. Introduction of mineral trioxide aggregate (MTA) has revolutionized such treatment.
Aim: The aim of our study was to evaluate and correlate the effects of MTA clinically and radiographically on pulpotomized primary molars till their exfoliation or extraction followed by histological evaluation.
Study design: This is an observational study.
Materials and methods: A total of 25 teeth were selected from 5- to 8-year-old children requiring pulp therapy on the basis of inclusion and exclusion criterion. The teeth were treated by conventional pulpotomy technique under aseptic conditions using MTA and were immediately restored with stainless steel crown. The teeth were assessed postoperatively till 36 months. The exfoliated or extracted teeth were examined histologically.
Results: The pulpotomized teeth were vital with no adverse clinical findings during the observation period. After 3 months, one tooth showed internal resorption, but the same was not observed after 12 months. Pulp canal obliteration was seen in three cases. At the end of the study, five teeth were exfoliated and one tooth was extracted for maintaining arch symmetry. The histological examination of extracted tooth revealed the presence of healthy pulp and the area of true calcification. Remaining exfoliated teeth presented dentin bridge formation.
Statistics: Frequencies and percentages were used for descriptive statistics. Fisher's exact tests were used to see the difference between clinical and radiological findings. The probability value was fixed at 5% level of significance.
Conclusion: The response of pulp in primary teeth to MTA was favorable in all cases from clinical and radiographic perspective, and histological evaluation confirmed the observation.
Milnes AR. Persuasive evidence that formocresol use in pedi-atric dentistry is safe. J Can Dent Assoc 2006 Apr;72(3):247-248.
Hingston EJ, Parmer S, Hunter ML. Vital pulpotomy in the primary dentition: attitudes and practices of community dental staff in Wales. Int J Paediatr Dent 2007 May:17(3):186-191.
Casas MJ, Kenny DJ, Judd PL, Johnston DH. Do we still need formocresol in pediatric dentistry? J Can Dent Assoc 2005 Nov;71(10):749-751.
Judd PL, Kenny DJ. Formocresol concerns: a review. J Can Dent Assoc 1987 May;53(5):401-404.
Shaw DW, Sheller B, Barras BD, Morton TH. Electrosurgical pulpotomy: a 6 months study in primates. J Endod 1987 Oct;13(10):500-505.
Ruemping DR, Morton TH Jr, Anderson MW. Electrosurgical pulpotomy in primates –a comparison with formocresol pulpotomy. Pediatr Dent 1983 Mar;5(1):14-18.
Shoji S, Nakamura M, Horiuchi H. Histopathological changes in dental pulps irradiated by CO2 laser: a preliminary report on laser pulpotomy. J Endod 1985 Sep;11(9):379-384.
García-Godoy F, Ranly DM. Clinical evaluation of pulpoto-mies with ZOE as the vehicle of glutaraldehyde. Pediatr Dent 1987 Jun;9(2):144-146.
Fuks AB, Bimstein E, Klein H, Guelmann M. Assessment of a 2% buffered glutaraldehyde solution in pulpotomized primary teeth of schoolchildren. ASDC J Dent Child 1990 Sep-Oct;57(5):371-375.
Fei AL, Udin RD, Johnson R. A clinical study of ferric sulfate as a pulpotomy agent in primary teeth. Pediatr Dent 1991 Nov-Dec;13(6):327-332.
Nakashima M. The induction of reparative dentine in the amputated dental pulp of the dog by bone morphogenetic protein. Arch Oral Biol 1990;35(7):493-497.
Rutherford RB, Wahle J, Tucker M, Roger D, Charette M. Induction of reparative dentine formation in monkeys by recombinant human osteogenic protein-1. Arch Oral Biol 1993 Jul;38(7):571-576.
Ranly DM. Pulpotomy therapy in primary teeth: new modalities for old rationales. Pediatr Dent 1994 Nov-Dec;16(6):403-409.
Lee SJ, Monsef M, Torabinejad M. Sealing ability of a mineral trioxide aggregate for repair of lateral root perforations. J Endod 1993 Nov;19(11):541-544.
Schwartz RS, Mauger M, Clement DJ, Walker WA III. Mineral trioxide aggregate: a new material for endodontics. J Am Dent Assoc 1999 Jul;130(7):967-975.
Aeinehchi M, Eslami B, Ghanbariha M, Saffar AS. Mineral trioxide aggregate (MTA) and calcium hydroxide as pulp capping agents in human teeth: a preliminary report. Int Endod J. 2003 Mar;36(3):225-231.
Chacko V, Kurikose S. Human pulpal response to mineral trioxide aggregate (MTA): a histologic study. J Clin Pediatr Dent 2006 Spring;30(3):203-210.
Caicedo R, Abbott PV, Alongi DJ, Alarcon MY. Clinical radio-graphic and histological analysis of the effects of mineral trioxide aggregate used in direct pulp capping and pulpoto-mies of primary teeth. Aust Dent J 2006 Dec;51(4):297-305.
Koh ET, Pittford TR, Torabinejad M, McDonold F. Mineral trioxide aggregate stimulates cytokine production in human osteoblasts. J Bone Min Res 1995;10S, S406.
Eidelman E, Holan G, Fuks AB. Mineral trioxide aggregate vs formocresol in pulpotomized primary molars: a preliminary report. Pediatr Dent 2001 Jan-Feb;23(1):15-18.
Agamy HA, Bakry NS, Mounir MM, Avery DR. Comparison of mineral trioxide aggreagate and formocresol as pulp capping agents in pulpotomized primary teeth. Pediatr Dent 2004 Jul-Aug;26(4):302-309.
Farsi N, Alamoudi N, Balto K, Mushayt A. Success of mineral trioxide aggregate in pulpotomized primary molars. J Clin Pediatr Dent 2005 Summer;29(4):307-311.
Moretti AB, Sakai VT, Oliveira TM, Fornetti AP, Santos CF, Machado MA, Abdo RC. The effectiveness of mineral trioxide aggregate, calcium hydroxide and formocresol for pulpoto-mies in primary teeth. Int Endod J 2008 Jul;41(7):547-555.
Subramaniam P, Konde S, Mathew S, Sugnani S. Mineral triox-ide aggregate as pulp capping agent for primary: 2 year follow-up study. J Clin Pediatr Dent 2009 Summer;33(4):311-314.
Godhi B, Sood PB, Sharma A. Effect of mineral trioxide and formocresol on vital pulp after pulpotomy of primary molars: An in vivo study. Contemp Clin Dent 2011 Oct;2(4):296-301.
Torabinejad M, Chivian N. Clinical applications of mineral trioxide aggregate. J Endod 1999 Mar;25(3):197-205.
Holan G, Eidelman E, Fuks AB. Long-term evaluation of pulp-otomy in primary molars using mineral trioxide aggregate or formocresol. Pediatr Dent 2005 Mar-Apr;27(2):129-136.
Jabbarifar SE, Khademi D, Ghasemi D. Success rate of formo-cresol in pulpotomy versus mineral trioxide aggregate in human primary molar tooth. J Res Med Sci 2004 Dec;6(6):304-307.
Smith NL, Seale NS, Nunn ME. Ferric sulfate pulpotomy in primary molars: a retrospective study. Pediatr Dent 2000 May-Jun;22(3):192-199.
Papagiannoulis L. Clinical studies on ferric sulphate as a pulpotomy medicaments in primary teeth. Eur J Paediatr Dent 2002 Sep;3(3):126-132.
Koh ET, McDonald F, Pittford TR, Torabinejad M. Cellular response to mineral trioxide aggregate. J Endod 1998 Aug; 24(8):543-547.
Seux D, Coulbe ML, Hartmann DJ, Gauthier JP, Magloire H. Odontoblast-like cytodifferentiation of human dental pulp cells in vitro in the presence of a calcium hydroxide-contain cement. Arch Oral Biol 1991;36(2):117-128.
Faraco IM Jr, Holland R. Response of pulp of dogs to capping with mineral trioxide aggregate or a calcium hydroxide cement. Dent Traumatol 2001 Aug;17(4):163-166.
Sonmez D, Sari S, Cetinbas T. A comparison of four pulpotomy techniques in primary molars: a long term follow-up. J Endod 2008 Aug;34(8):950-955.
Iwamoto CE, Erika A, Pameijer CH, Barnes D, Romberg EE, Jefferies S. Clinical and histological evaluation of white Pro Root MTA in direct pulp capping. Am J Dent 2006 Apr;19(2):85-90.
Chueh LH, Chiang CP. Histology of irreversible pulpitis pre-molars treated with mineral trioxide aggregate pulpotomy. Oper Dent 2010 May-Jun;35(3):370-374.
Wongprasartsuk P, Stevens J. Cerebral palsy and anaesthesia. Paediatr Anaesth 2002 May;12(4):296-303.
Verschuren O, Wiart L, Ketelaar M. Stages of change in physical activity behavior in children and adolescents with cerebral palsy. Disabil Rehabil 2013 Jan;35(19):1630-1635.
Surén P, Bakken IJ, Aase H, Chin R, Gunnes N, Lie KK, Magnus P, Reichborn-Kjennerud T, Schj⊘lberg S, Øyen AS, et al. Autism spectrum disorder, ADHD, epilepsy, and cerebral palsy in norwegian children. Pediatrics 2012 Jul;130(1):e152-e158.
Pollare T, Lithell H, Selinus I, Berne C. Application of prazosin is associated with an increase of insulin sensitivity in obese patients with hypertension. Diabetologia 1988 Jul;31(7):415-420.
Berne C, Pollare T, Lithell H. Effects of antihypertensive treatment on insulin sensitivity with special reference to ACE inhibitors. Diabetes Care 1991 Nov;14(Suppl 4):39-47.
Cohn JN. Relationship of plasma volume changes to resistance and capacitance vessel effects of sympathomimetic amines and angiotensin in man. Clin Sci 1966 Apr;30(2):267-278.
Julius S, Pascual AV, Reilly K, London R. Abnormalities of plasma volume in borderline hypertension. Arch Intern Med 1971 Jan;127(1):116-119.
Greenwood JP, Scott EM, Stoker JB, Mary DA. Hypertensive left ventricular hypertrophy: relation to peripheral sympathetic drive. J Am Coll Cardiol 2001 Nov;38(6):1711-1717.
Spirito P, Bellone P, Harris KM, Bernabo P, Bruzzi P, Maron BJ. Magnitude of left ventricular hypertrophy and risk of sudden death in hypertrophic cardiomyopathy. N Engl J Med 2000 Jun;342:1778-1785.
Haider AW, Larson MG, Benjamin EJ, Levy D. Increased left ventricular mass and hypertrophy are associated with increased risk for sudden death. J Am Coll Cadiol 1998 Nov;32(5):1454-1459.
Jouven X, Zureik M, Desnos M, Guérot C, Ducimetière P. Resting heart rate as a predictive factor for sudden death in middle-aged men. Cardiovasc Res 2001 May;50(2):373-378.
Gillman MW, Kannel WB, Belanger A, D'Agostino RB. Influence of heart rate on mortality among persons with hypertension: the Framingham Study. Am Heart J 1993 Apr;125(4):1148-1154.
Julius S, Palatini P, Kjeldsen SE, Zanchetti A, Weber MA, McInnes GT, Brunner HR, Mancia G, Schork MA, Hua TA, et al. Usefulness of heart rate to predict future cardiac events in treated patients with high-risk systemic hypertension. Am J Cardiol 2012 Mar;109(5):685-692.
Palatini P, Roawi W, Casiglia E, Chalmers J, Ferrari R, Grassi G, Inoui T, Jelakovic B, Jensen MT, Julius S, et al. Management of the hypertensive patient with elevated heart rate: Statement of the Second Consensus Conference endorsed by the European Society of Hypertension. J Hypertens 2016 May;34(5):813-821.
Benedict CR, Shelton B, Johnstone DE, Francis G, Greenberg B, Konstam M, Probstfield JL, Yusuf S. Prognostic significance of plasma norepinephrine in patients with asymptomatic left ventricular dysfunction. Circulation 1996 Aug;94(4):690-697.
Grassi G, Seravalle G, Mancia G. Sympathetic activation in cardiovascular disease: evidence, clinical impact and therapeutic implications. Eur J Clin Invest 2015 Dec;45(12):1367-1375.
Cullington D, Goode KM, Zhang J, Cleland JG, Clark AL. Is heart rate important for patients with heart failure in atrial fibrillation? JACC Heart Fail 2014 Jun;2(3):213-220.
Wikstrand J, HJalmarson A, Waagstein F, Fagerberg J, Goldstein S, Kjekshus J, Wedel H; MERIT-HF Study Group. Dose of metoprolol CR/XL and clinical outcomes in patients with heart failure: analysis of the experience in metoprolol CR/XL randomized intervention trial in chronic heart failure (MERIT-HF). J Am Coll Cardiol 2002 Aug;40(3):491-498.
Yancy CW, Jessup M, Bozkurt B, Bulter J, Casey DE Jr, Colvin MM, Drazner MH, Filippatos GS, Fonarow GC, Givertz MM, et al. 2017 ACC/AHA/HFSA focused update of the 2013 ACCF/AHA guideline for the management of heart failure: A Report of the American College of Cardiology/American Heart Association Task Force on Clinical Practice Guidelines and the Heart Failure Society of America. J Am Coll Cardiol 2017 Aug;70(6):776-803.
Luetic AT. First experience in clinical application of KANET. Donald School J Ultrasound Obstet Gynecol 2015;9(1):96-99.
Vasilj O. Is in utero fetal neurological assessment comparible to postnatal neurological assessment? Donald School J Ultrasound Obstet Gynecol 2015;9(1):91-95.
Tomasovic S, Predojevic M. 4D ultrasound medical devices for recent advances on the etiology of cerebral palsy. Acta Inform Med 2011 Dec;19(4):228-234.
Vladareanu R, Lebit D, Constantinescu S. Ultrasound assessment of fetal neurobehavior in high-risk pregnancies. Donald School J Ultrasound Obstet Gynecol 2012 Apr-Jun;6(2):132-147.
Honemeyer U, Talic A, Therwat A, Paulose I, Patidar R. The clinical value of KANET test in studying fetal neurobehavior in normal and at-risk pregnancies. J Perinat Med 2013 Mar;41(2):187-197.
Kadic AS. Fetal neurology: the role of fetal stress. Donald School J Ultrasound Obstet Gynecol 2015;9(1):30-39.
Hata T, Kanenishi J, Hanoka U, Uematsu R, Marumo G, Tanaka H. HDlive study of fetal development and behavior. Donald School J Ultrasound Obstet Gynecol 2014;8(3):250-265.
Reynoso C, Crespo-Eguílaz N, Alcázar JL, Narbona J. Motor behavior of human fetuses during the second trimester of gestation: a longitudinal ultrasound study. An Pediatr (Barc) 2015 Mar;82(3):183-191.
Predojević M, Talić A, Stanojević M, Kurjak A, Salihagić Kadić A. Assessment of motoric and hemodynamic parameters in growth restricted fetuses-case study. J Matern Fetal Neonatal Med 2014 Feb;27(3):247-251.
Kurjak A, Talic A, Stanojevic M, Honemeyer U, Serra B, Prats P, Di Renzo GC. The study of fetal neurobehavior in twins in all three trimesters of pregnancy. J Matern Fetal Neonatal Med 2013 Aug;26(12):1186-1195.
Athanasiadis AP, Mikos T, Tambakoudis GP, Theodoridis TD, Papastergiou M, Assimakopoulos E, Tarlatzis BC. Neuro-developmental fetal assessment using Kanet scoring system in low and high-risk pregnancies. J Matern Fetal Neonatal Med 2013;26(4):363-368.
Kurjak A, Stanojević M, Predojević M, Laušin I, Salihagić-Kadić A. Neurobehavior in fetal life. Semin Fetal Neonatal Med 2012 Dec;17(6):319-323.
Hepper PG, Dornan JC, Lynch C, Maguire JF. Alcohol delays the emergence of the fetal elicited startle response, but only transiently. Physiol Behav 2012 Aug 20;107(1):76-81.
Talic A, Kurjak A, Stanojevic M, Honemeyer U, Badreldeen A, Direnzo GC. The assessment of fetal brain function in fetuses with ventriculomegaly: the role of the Kanet test. J Matern Fetal Neonatal Med 2012 Aug;25(8):1267-1272.
Kurjak A, Honemeyer U, Stanojevic M, Zalud I. Comparison between antenatal neurodevelopmental test and fetal Doppler in the assessment of fetal well-being. J Perinat Med 2013 Jan;41(1):107-114.
Neto MR. KANET in Brazil: first experience. Donald School J Ultrasound Obstet Gynecol 2015;9(1):1-5.
Morokuma S, Fukushima K, Yumoto Y, Uchimura M, Fujiwara A, Matsumoto M, Satoh S, Nakano H. Simplified ultrasound screening for fetal brain function based on behavioral pattern. Early Hum Dev 2007 Mar;83(3):177-181.
Kurjak A, Tikvica Luetic A, Stanojevic M, Talic A, Zalud I, Al-Noobi M, Perva S, Abushama M, Tomasovic S, Zaputovic S, et al. Further experience in the clinical assessment of fetal neurobehavior. Donald School J Ultrasound Obstet Gynecol 2010 Jan-Mar;4(1):59-71.
Kurjak A, Abo-Yaqoub S, Stanojevic M, Yigiter AB, Vasilj O, Lebit D, Shaddad AN, Ahmed B, Kavak ZN, Miskovic B, et al. The potential of 4D sonography in the assessment of fetal neurobehavior: multicentric study in high-risk pregnancies. J Perinat Med 2010;38(1):77-82.
Miskovic B, Vasilj O, Stanojevic M, Ivankovicc D, Kerner M, Tikvica A. The comparison of fetal behavior in high-risk and normal pregnancies assessed by four-dimensional ultrasound. J Matern Fetal Neonatal Med 2010 Dec;23(12):1461-1467.
Kurjak A, Ahmed B, Abo-Yaguab S, Younis M, Saleh H, Shaddad AN, Vasilj O, Al Bahar AJ, Miskovic B, Khenyab N. An attempt to introduce neurological test for fetus based on 3D and 4D sonography. Donald School J Ultrasound Obstet Gynecol 2008;2:29-44.
Livingston JH, Stivaros S, Warren D, Crow YJ. Intracranial calcification in childhood: a review of aetiologies and recognizable phenotypes. Dev Med Child Neurol 2014 Jul;56(7):612-626.
Couvreur J, Desmonts G, Tournier G, Szusterkac M. A homogeneous series of 210 cases of congenital toxoplasmosis in 0 to 11-month-old infants detected prospectively. Ann Pediatr (Paris) 1984 Nov;31(10):815-819. (French).
Valentini P, Annunziata ML, Angelone DF, Masini L, De Santis M, Testa A, Grillo RL, Speziale D, Ranno O. Role of spiramycin/cotrimoxazole association in the mother-to-child transmission of toxoplasmosis infection in pregnancy. Eur J Clin Microbiol Infect Dis 2009 Mar;28(3):297-300.
Valentini P, Buonsenso D, Barone G, Serranti D, Calzedda R, Ceccarelli M, Speziale D, Ricci R, Masini L. Spiramycin/cotrimoxazole versus pyrimethamine/sulfonamide and spiramycin alone for the treatment of toxoplasmosis in pregnancy. J Perinatol 2015 Feb;35(2):90-94.
Masini L, De Santis M, Apicella M, Noia G, Santangelo R, Speziale D, Lanzone A, Valentini P. Prenatal diagnosis by ultrasound and amniocentesis in a single center cohort of 1065 pregnancies complicated by maternal Toxoplasma infection. ISUOG 2017—27th World Congress—16-19 September, Vienna, Austria OC05.05. Ultrasound Obstet Gynecol 2017;50(Suppl 1):10.
Vynnycky E, Adams EJ, Cutts FT, Reef SE, Navar AM, Simons E, Yoshida LM, Brown DW, Jackson C, Strebel PM, et al. Using seroprevalence and immunisation coverage data to estimate the global burden of congenital rubella syndrome, 1996-2010: a systematic review. PLoS One 2016 Mar 10;11(3):e0149160.
Hutton J. Does rubella Cause autism: a 2015 reappraisal? Front Hum Neurosci 2016 Feb 1;10:25.
Thompson KM, Simons EA, Badizadegan K, Reef SE, Cooper LZ. Characterization of the risks of adverse outcomes following rubella infection in pregnancy. Risk Anal 2016 Jul;36(7):1315-1331.
De Santis M, Cavaliere AF, Straface G, Caruso A. Rubella infection in pregnancy. Reprod Toxicol 2006 May;21(4):390-298.
Harger JH, Ernest JM, Thurnau GR, Moawad A, Thom E, Landon MB, Paul R, Miodovnik M, Dombrowski M, Sibai B, et al. Frequency of congenital varicella syndrome in a prospective cohort of 347 pregnant women. Obstet Gynecol 2002 Aug;100(2):260-265.
Enders G, Miller E, Cradock-Watson J, Bolley I, Ridehalgh M. Consequences of varicella and herpes zoster in pregnancy: prospective study of 1739 cases. Lancet 1994 Jun 18;343(8912):1548-1551.
Tan MP, Koren G. Chickenpox in pregnancy: revisited. Reprod Toxicol 2006 May;21(4):410-420.
Pastuszak AL, Levy M, Schick B, Zuber C, Feldkamp M, Gladstone J, Bar-Levy F, Jackson E, Donnenfeld A, Meschino W, et al. Outcome after maternal varicella infection in the first 20 weeks of pregnancy. N Engl J Med 1994 Mar 31;330(13):901-905.
Pretorius DH, Hayward I, Jones KL, Stamm E. Sonographic evaluation of pregnancies with maternal varicella infection. J Ultrasound Med 1992 Sep;11(9):459-463.
Verstraelen H, Vanzieleghem B, Defoort P, Vanhaesebrouck P, Temmerman M. Prenatal ultrasound and magnetic resonance imaging in fetal varicella syndrome: correlation with pathology findings. Prenat Diagn 2003 Sep;23(9):705-709.
Paryani SG, Arvin AM. Intrauterine infection with varicella-zoster virus after maternal varicella. N Engl J Med 1986 Jun 12;314(24):1542-1546.
Meyberg-Solomayer GC, Fehm T, Muller-Hansen I, Enders G, Poets C, Wallwiener D, Solomayer EF. Prenatal ultrasound diagnosis, follow-up, and outcome of congenital varicella syndrome. Fetal Diagn Ther 2006;21(3):296-301.
Pichler M, Staffler A, Bonometti N, Messner H, Deluca J, Thuile T, Kluge R, Schmuth M, Eisendle K. Premature newborns with fatal intrauterine herpes simplex virus-1 infection: first report of twins and review of the literature. J Eur Acad Dermatol Venereol 2015 Jun;29(6):1216-1220.
Plourde AR, Bloch EM. A literature review of Zika virus. Emerg Infect Dis 2016 Jul;22(7):1185-1192.
Paixão ES, Barreto F, Teixeira Mda G, Costa Mda C, Rodrigues LC. History, epidemiology, and clinical manifestations of Zika: a systematic review. Am J Public Health 2016 Apr;106(4):606-612.
Rasmussen SA, Jamieson DJ, Honein MA, Petersen LR. Zika virus and birth defects—reviewing the evidence for causality. N Engl J Med 2016 May 19;374(20):1981-1987.
Honein MA, Dawson AL, Petersen EE, Jones AM, Lee EH, Yazdy MM, Ahmad N, Macdonald J, Evert N, Bingham A, et al. Birth defects among fetuses and infants of US women with evidence of possible Zika virus infection during pregnancy. JAMA 2017 Jan 3;317(1):59-68.
Chervenak FA, Jeanty P, Cantraine F, Chitkara U, Venus I, Berkowitz RL, Hobbins JC. The diagnosis of fetal microcephaly. Am J Obstet Gynecol 1984 Jul 1;149(5):512-517.
Papageorghiou AT, Thilaganathan B, Bilardo CM, Ngu A, Malinger G, Herrera M, Salomon LJ, Riley LE, Copel JA. ISUOG Interim Guidance on ultrasound for Zika virus infection in pregnancy: information for healthcare professionals. Ultrasound Obstet Gynecol 2016 Apr;47(4):530-532.
Chibueze EC, Parsons AJ, Lopes KD, Yo T, Swa T, Nagata C, Horita N, Morisaki N, Balogun OO, Dagvadorj A, et al. Diagnostic accuracy of ultrasound scanning for prenatal microcephaly in the context of Zika virus infection: a systematic review and meta-analysis. Sci Rep 2017 May 23;7(1):2310.
Cordeiro MT. Zika virus: laboratory diagnosis. In: Zika in focus. Springer International Publishing; 2017. pp. 59-62.
Mlakar J, Korva M, Tul N, Popović M, Poljšak-Prijatelj M, Mraz J, Kolenc M, Resman Rus K, Vesnaver Vipotnik T, Fabjan Vodušek V, et al. Zika virus associated with microcephaly. N Engl J Med 2016 Mar 10;374(10):951-958.
Zare Mehrjardi M, Keshavarz E, Poretti A, Hazin AN. Neuroimaging findings of Zika virus infection: a review article. Jpn J Radiol 2016 Dec;34(12):765-770.
Oliveira Melo AS, Malinger G, Ximenes R, Szejnfeld PO, Alves Sampaio S, Bispo de Filippis AM. Zika virus intrauterine infection causes fetal brain abnormality and microcephaly: tip of the iceberg? Ultrasound Obstet Gynecol 2016 Jan;47(1):6-7.
Brasil P, Pereira JP Jr, Moreira ME, Ribeiro Nogueira RM, Damasceno L, Wakimoto M, Rabello RS, Valderramos SG, Halai UA, Salles TS, et al. Zika virus infection in pregnant women in Rio de Janeiro. N Engl J Med 2016 Dec 15;375(24):2321-2334.