Aims and background: This study aimed to assess the accuracy of digital intraoral scans in capturing the three-dimensional (3D) surface of teeth and dental arches in mixed dentition, compared with conventional plaster models. Intraoral scanning technology has seen rapid advancements in recent years, revolutionizing orthodontic and dental practices. However, its accuracy in mixed dentition remains a subject of investigation. Materials and methods: Children with mixed dentition (n = 34) were selected according to specific criteria. Digital intraoral scanning was performed using the Medit i500, and conventional plaster models were created following alginate impression techniques. The arch widths on the 3D digital models were measured using Medit Link software, while the conventional models were measured using calipers. Both sets of data were then analyzed using Moyer's analysis. Results: The results of the study showed no significant difference (p > 0.05) between the mean space analysis measurements obtained conventionally and digitally in the mixed dentition phase using Moyer's analysis method. Conclusion: Despite minor discrepancies compared to conventional plaster models, digital intraoral scans offer a clinically acceptable level of accuracy for orthodontic diagnosis and treatment planning. The benefits of digital scanning, including improved patient experience and enhanced digital workflows, make it a promising tool for modern dental and orthodontic practices. Clinical significance: There is no significant difference between space analysis measurements in the mixed dentition phase using Moyer's analysis on conventional and 3D digital models.