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VOLUME 17 , ISSUE 9 ( September, 2024 ) > List of Articles

ORIGINAL RESEARCH

Effect of Herbal Extract of Spilanthes acmella and Cinnamon Oil on Enterococcus faecalis Biofilm Eradication: An In Vitro Study

Shruti A Bhamare, Prasanna T Dahake, Yogesh J Kale, Mahesh V Dadpe, Shrikant B Kendre

Keywords : Antibacterial, Antimicrobial efficacy, Biofilm, Calcium hydroxide, Cinnamon oil, Root canal therapy, Virulence factor

Citation Information : Bhamare SA, Dahake PT, Kale YJ, Dadpe MV, Kendre SB. Effect of Herbal Extract of Spilanthes acmella and Cinnamon Oil on Enterococcus faecalis Biofilm Eradication: An In Vitro Study. Int J Clin Pediatr Dent 2024; 17 (9):1004-1013.

DOI: 10.5005/jp-journals-10005-2922

License: CC BY-NC 4.0

Published Online: 30-09-2024

Copyright Statement:  Copyright © 2024; The Author(s).


Abstract

Introduction: Enterococcus faecalis has a pathogenic role in failed endodontic treatments. The study aimed to assess the efficiency of Spilanthes acmella (SA) and cinnamon oil (CO) extract on E. faecalis biofilm eradication. Materials and methods: The antibacterial efficacy of SA and CO against E. faecalis was assessed by the tests of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC), and further, the interaction with agents was evaluated at different time intervals by a time-kill assay. The inhibition efficacy of both agents was determined by biofilm adhesion reduction crystal violet assay. Results: The MIC of SA was 25 μg/mL, and for CO, it was 12.5 μg/mL. The time-kill assay revealed that antibacterial efficacy was identified till 36 hours by both the test materials. The mean biofilm reduction at 25 µg/mL of calcium hydroxide [Ca(OH)2], SA, and CO was 1.53 ± 0.05, 1.83 ± 1.57, and 2.06 ± 0.05, respectively. Conclusion: SA and CO demonstrated promising antibacterial efficacy against E. faecalis and CO presented significant eradication of biofilms compared to SA.

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