Citation Information :
Manohar R, Venkatesan K, Raja S, Ganesh A, Kanakasabapathy BS. Assessment of Microbial Contamination of a Toothbrush Head with and without a Protective Cover: An Ex Vivo Study. Int J Clin Pediatr Dent 2022; 15 (4):455-457.
Introduction: The oral cavity is colonized by various groups of microorganisms. Toothbrushing is a common method of maintaining oral hygiene and, upon regular use, can become heavily contaminated with microorganisms. To avoid contamination of the toothbrush with microorganisms from the external environment, toothbrushes can be protected by a protective cap, the significance of which is unknown. Objectives: To assess the microbial contamination of the toothbrush with and without a protective cap and to find the significance of the same against microbial contamination. Materials and methods: An ex vivo study was conducted in the Faculty of Dental Sciences, Sri Ramachandra University. A total of 40 toothbrushes were distributed among dental students aged 18–25 years; 20 were protected by a cap, 20 were unprotected, and instructions were given to recap the toothbrush after brushing. After 1 month of regular usage, toothbrushes were collected, and organisms were identified based on Gram's reaction followed by a biochemical test. Results: From the study conducted, it is evident that the microbial contamination of the unprotected toothbrush is higher than that of the toothbrushes that were protected with a cover.
Karibasappa GN, Nagesh L, Sujatha BK. Assessment of microbial contamination of toothbrush head: an in vitro study. Indian J Dent Res 2011;22(1):2–5. DOI: 10.4103/0970-9290.79965
Filho PN, Macari S, Faria G, et al. Microbial contamination of toothbrushes and their decontamination. Pediatr Dent 2000;22(5):381–384. https://www.aapd.org/globalassets/media/publications/archives/filho-22-05.pdf
Himratul-Aznita WH, Raja Dzulkeffli RNS, Muhadi SH. Isolation and antibiotic resistance of microorganisms from toothbrush. Res J Biol Sci 2007;2(1):54–56. https://docsdrive.com/pdfs/medwelljournals/rjbsci/2007/54-56.pdf
Taji SS, Rogers AH. The microbial contamination of toothbrushes. A pilot study. Aus Dent J 1998;43(2);128–130. DOI: 10.1111/j.1834-7819.1998.tb06101.x
Contreras A, Arce R, Botero JE, et al. Toothbrush contamination in family members. Rev Clin Periodoncia Implantol Rehabil Oral 2010;3(1):24–26. DOI: 10.4067/S0719-01072010000100004
Sogi SHP, Subbareddy VV, Kiran SND. Contamination of toothbrush at different time intervals and effectiveness of various disinfecting solutions in reducing the contamination of toothbrush. J Indian Sot Pedod Prev Dent 2002;20(3):81–85. https://www.jisppd.com/article.asp?issn=0970-4388;year=2002;volume=20;issue=3;spage=81;epage=5;aulast=Sogi;type=0
Sato S, Ito IY, Lara EHG, et al. Bacterial survival rate on toothbrushes and their decontamination with antimicrobial solutions. J Appl Oral Sci 2004;12(2):99–103. DOI: 10.1590/s1678-77572004000200003
Frazelle MR, Munro CL. Toothbrush contamination: a review of the literature. Nurs Res Pract 2012:420630. DOI: 10.1155/2012/420630
Konidala U, Nuvvula S, Mohapatra A, et al. Efficacy of various disinfectants on microbially contaminated toothbrushes due to brushing. Contemp Clin Dent 2011;2(4);302–307. DOI: 10.4103/0976-237X.91793
Thamke MV, Beldar A, Thakkar P, et al. Comparison of bacterial contamination and antibacterial efficacy in bristles of charcoal toothbrushes versus noncharcoal toothbrushes: a microbiological study. Contemp Clin Dent 2018;9(3):463–467. DOI: 10.4103/ccd.ccd_309_18
Do Nascimento C, Scarabel TT, Miani PK, et al. In vitro evaluation of the microbial contamination on new toothbrushes: a preliminary study. Microsc Res Tech 2012;75(1):42–45. DOI: 10.1002/jemt.21020