International Journal of Clinical Pediatric Dentistry

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VOLUME 14 , ISSUE 5 ( September-October, 2021 ) > List of Articles

RESEARCH ARTICLE

Efficacy of Silver Diamine Fluoride on Streptococcus mutans Count Present in Saliva

Sarbojit Chakraborty, Nidhi Gupta, Natasha Gambhir, Rashi Singh

Keywords : 38% SDF, Carious primary tooth, Silver diamine fluoride, Streptococcus mutans

Citation Information : Chakraborty S, Gupta N, Gambhir N, Singh R. Efficacy of Silver Diamine Fluoride on Streptococcus mutans Count Present in Saliva. Int J Clin Pediatr Dent 2021; 14 (5):700-704.

DOI: 10.5005/jp-journals-10005-2037

License: CC BY-NC 4.0

Published Online: 20-11-2021

Copyright Statement:  Copyright © 2021; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

Aim and objective: Evaluate the efficacy and longevity of silver diamine fluoride (SDF) to inhibit the dental caries initiator bacteria; Streptococcus mutans count in saliva. Study design: Twenty children having class I and II dental caries with no prior restorations were included in the study. Unstipulated saliva samples were collected before and immediately after application of SDF, 1, 3, and 6 months after application, and intergroup comparison was done. Materials and methods: Silver diamine fluoride was applied on the cavitated surfaces with microbrush tip under isolation, then wait for 4 minutes and washed away all surfaces with water spray. Saliva was collected in a sterile saliva collection tube and sent for microbiological culture assessment. CFU/mL of S. mutans count was done with the Digital colony counter. For statistical analysis, paired T-test was performed. Results: Silver diamine fluoride showed a significant drop in CFU/mL of S. mutans count at 1 month then 3 and 6 months accordingly. Conclusion: Silver diamine fluoride can be used to prevent and arrest dental caries and the best result can be achieved by quarterly application.


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  1. Bowen WH, Birkhed D. Dental caries: dietary and microbiology factors. In: Granath L, McHugh WD, ed. Systemized prevention of oral disease: theory and practice. Boca Raton (FL): CRC Press, Inc.; 1986. pp. 19–41.
  2. Van Houte J. Role of microorganisms in caries etiology. J Dent Res 1994;73(3):672–681. DOI: 10.1177/00220345940730031301.
  3. Loesche WJ. Role of Streptococcus mutans in human dental decay. Microbiol Rev 1986;50(4):353–380. DOI: 10.1128/mr.50.4.353-380.1986.
  4. Marsh PD. The significance of maintaining the stability of the natural micro flora of the mouth. Br Dent J 1991;17(6):174–177. DOI: 10.1038/sj.bdj.4807647.
  5. Chosak A, Cleaton-Jones P, Woods A, et al. Caries prevalence and severity in the primary dentition and Streptococcus mutans level in saliva of preschool children in South Africa. Community Dent Oral Epidemiol 1988;16(5):289–291. DOI: 10.1111/j.1600-0528.1988.tb01777.x.
  6. Mattos-Silveira J, Floriano I, Ferreira FR, et al. New proposal of silver diamine fluoride use in arresting approximal caries: study protocol for a randomized controlled trial. Trials 2014;15(1):448. DOI: 10.1186/1745-6215-15-448.
  7. Mei ML, Chu CH, Low KH, et al. Caries arresting effect of silver diamine fluoride on dentine carious lesion with S. mutans and L. acidophilus dual-species cariogenic biofilm. Med Oral Patol Oral Cir Bucal 2013;18(6):e824–e831. DOI: 10.4317/medoral.18831.
  8. Newbrun E. Sugar and dental caries: a review of human studies. Science 1982;217(4558):418–423. DOI: 10.1126/science.7046052.
  9. Islam B, Khan SN, Khan AU. Dental caries: from infection to prevention. Med Sci Monit 2007;13(11):RA196–RA203.
  10. Slavkin HC. Streptococcus mutans, early childhood caries and new opportunities. J Am Dent Assoc 1999;130(12):1787–1792. DOI: 10.14219/jada.archive.1999.0138.
  11. Rosenblatt A, Stamford TCM, Niederman R. Silver diamine fluoride: a caries “silver fluoride bullet”. J Dent Res 2009;88(2):116–125. DOI: 10.1177/0022034508329406.
  12. Yee R, Holmgren C, Mulder J, et al. Efficacy of silver diamine fluoride for arresting caries treatment. J Dent Res 2009;88(7):644–647. DOI: 10.1177/0022034509338671.
  13. Featherstone JD, Horst JA. Long used in other countries, silver diamine fluoride is now cleared for use in the U.S. While there is an assumption of use for pediatric caries control, here we explore indications for adults. J Multidisciplin Care Decis Dentis 2015. 1–8. Retrieved from Pubmed.
  14. Braga MM, Mendes FM, De Benedetto MS, et al. Effect of silver diammine fluoride on incipient caries lesions in erupting permanent first molars: a pilot study. J Dent Child 2009;76(1):28–33.
  15. Nelson T, Scott JM, Crystal YO, et al. Silver diamine fluoride in pediatric dentistry training programs: survey of graduate program directors. Pediatr Dent 2016;38(3):212–217.
  16. Naegeli C. On the oligodynamic phenomenon in living cells. Denkschriften der Schweizerischen Naturforschenden Gesellschaft 1893;33:174–182.
  17. Howe PR. A method of sterilizing and at the same time impregnating with a metal affected dentinal tissue. Dent Cosm 1917;59:891–904.
  18. Gao SS, Zhao IS, Duffin S, et al. Revitalising silver nitrate for caries management. Int J Environ Res Public Health 2018;15(1):80. DOI: 10.3390/ijerph15010080.
  19. Craig G, Powell K, Cooper M. Caries progression in primary molars: 24-month results from a minimal treatment programme. Community Dentis Oral Epidemiol 1982;9(6):260–265. DOI: 10.1111/j.1600-0528.1981.tb00342.x.
  20. Shah S, Bhaskar V, Venkatraghavan K, et al. Silver diamine fluoride: a review and current applications. J Adv Oral Res 2014;5(1):25–35. DOI: 10.1177/2229411220140106.
  21. Fung MHT, Wong MCM, Lo ECM, et al. Arresting early childhood caries with silver diamine fluoride-a literature review. J Oral Hyg Health 2013;1(03):117. DOI: 10.4172/2332-0702.1000117.
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