International Journal of Clinical Pediatric Dentistry

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VOLUME 14 , ISSUE 2 ( March-April, 2021 ) > List of Articles


Antimicrobial Efficacy of Commercially Available Low-fluoride and Fluoride-free Dentifrices for Children

Divya Reddy, Arul Selvan

Citation Information : Reddy D, Selvan A. Antimicrobial Efficacy of Commercially Available Low-fluoride and Fluoride-free Dentifrices for Children. Int J Clin Pediatr Dent 2021; 14 (2):183-186.

DOI: 10.5005/jp-journals-10005-1915

License: CC BY-NC 4.0

Published Online: 30-07-2021

Copyright Statement:  Copyright © 2021; The Author(s).


Aim and objective: The objective of this study was to evaluate and compare the antimicrobial efficacy of low-fluoride and fluoride-free dentifrices against Streptococcus mutans. Materials and methods: The antimicrobial efficacy of four commercially available low-fluoride child formula dentifrices and four fluoride-free dentifrices against S. mutans was determined using the agar diffusion test. Fifty microliters of various dilutions (1:1, 1:2, 1:4) of each dentifrice were inoculated on the assigned plates under aseptic conditions. Saline was taken as negative control and 0.2% chlorhexidine was considered as a positive control. The plates were incubated at 37°C for 24 hours and the zone of inhibition around the wells was measured. Results: All the tested low-fluoride dentifrices showed varying degrees of antimicrobial activity against S. mutans with F2 (Pediflor®) and F4 (Cheerio™) showing greater zones of inhibition when compared to F1 (Colgate®kids) and F3 (Kidodent). When the mean zones of inhibition produced by non-fluoridated dentifrices were compared with that of fluoridated dentifrices, no statistically significant difference was noted between NF1, NF3, NF4, and F2, F4. The antibacterial activity of F1 and F3 was significantly lower when compared to others. However, no antibacterial activity was noted with NF2. Conclusion: Both low-fluoride and fluoride-free formulations tested in the study exhibited antimicrobial activity against S. mutans. In very young children where the risk of fluorosis is of concern, fluoride-free formulations can be considered as safe alternatives to fluoride formulations. Clinical significance: Several dentifrices, both fluoride-free and low-fluoride formulations, are being aggressively marketed for young children. Though these toothpastes are being very commonly used by young parents for their infants and toddlers, there is very little published literature available on their antimicrobial activity and this study focuses on addressing this.

  1. Van Houte J. Role of microorganisms in caries etiology. J Dent Res 1994;73(3):672–681. DOI: 10.1177/00220345940730031301.
  2. Holt RD, Murray JJ. Developments in fluoride toothpastes: an overview. Community Dent Health 1997;14(1):4–10.
  3. Levy SM. A review of fluoride intake from fluoride dentifrice. ASDC J Dent Child 1993;60(2):115–124.
  4. Beltran ED, Szpunar SM. Fluoride in toothpastes for children: suggestion for change. Pediatr Dent 1988;10(3):185–188.
  5. Stookey GK. Review of fluorosis risk of self-applied topical fluorides: dentifrices, mouthrinses and gels. Community Dent Oral Epidemiol 1978;6(5):222–226. DOI: 10.1111/j.1600-0528.1978.tb01154.x.
  6. Malhotra R, Singla S, Shashikiran ND. Comparison of antimicrobial activity of child formula dentifrices at different concentrations: an in vitro study. Int J Clin Pediatr Dent 2017;10(2):131–135. DOI: 10.5005/jp-journals-10005-1422.
  7. Lodaya R, Venkataraman S, Lakade L, et al. Comparative evaluation of antimicrobial efficiency of marheted children's fluoridated toothpastes at diluted concentrations against Streptococcus mutans – an in vitro study. Int Dent Med J Adv Res 2018;4(1):1–5. DOI: 10.11648/j.ijdm.20180401.11.
  8. Reed MW. Clinical evaluation of three concentrations of sodium fluoride in dentifrices. J Am Dent Assoc 1973;87(7):1401–1403. DOI: 10.14219/jada.archive.1973.0618.
  9. Peterson LG, Birkhed D, Gleerup A, et al. Caries-preventive effect of dentifrices containing various types and concentrations of fluorides and sugar alcohols. Caries Res 1991;25(1):74–79. DOI: 10.1159/000261346.
  10. Randall JP, Seow WK, Walsh LJ. Antibacterial activity of fluoride compounds and herbal toothpastes on Streptococcus mutans: an in vitro study. Aust Dent J 2015;60(3):368–374. DOI: 10.1111/adj. 12247.
  11. Roopavathi KM, Gopal SV, Pushpalatha G, et al. Antimicrobial efficacy of commercially available toothpastes – an in vitro study. J Young Pharm 2015;7(3):187–193. DOI: 10.5530/jyp.2015.3.8.
  12. Takahashi-Abbe S, Abbe K, Takahashi N, et al. Inhibitory effect of sorbitol on sugar metabolism of Streptococcus mutans in vitro and on acid production in dental plaque in vivo. Oral Microbiol Immunol 2001;16(2):94–99. DOI: 10.1034/j.1399-302x.2001.016002094.x.
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