International Journal of Clinical Pediatric Dentistry

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VOLUME 13 , ISSUE 6 ( November-December, 2020 ) > List of Articles

Original Article

Antibacterial Efficacy of Sodium Hypochlorite, Ozonated Water, and 980 nm Diode Laser Used for Disinfection of Root Canal against Enterococcus faecalis: A Microbiological Study

Jagriti Kushwah, Rahul Mishra, Vishal Bhadauria

Keywords : 980 nm diode laser, Enterococcus faecalis, Ozonated water, Sodium hypochlorite

Citation Information : Kushwah J, Mishra R, Bhadauria V. Antibacterial Efficacy of Sodium Hypochlorite, Ozonated Water, and 980 nm Diode Laser Used for Disinfection of Root Canal against Enterococcus faecalis: A Microbiological Study. Int J Clin Pediatr Dent 2020; 13 (6):694-699.

DOI: 10.5005/jp-journals-10005-1860

License: CC BY-NC 4.0

Published Online: 31-03-2021

Copyright Statement:  Copyright © 2020; The Author(s).


Abstract

Aim: Evaluation and comparison of the antibacterial efficacy of sodium hypochlorite, ozonated water, diode laser, and diode laser in combination with ozonated water against Enterococcus faecalis. Materials and methods: One hundred and twenty extracted premolar teeth were sectioned at the cementoenamel junction and root canals were prepared using step-back technique with K-file up to #40. The teeth were arbitrarily allocated to four groups and stowed into brain heart infusion broth containing a microbial suspension of E. faecalis. Group I samples were irrigated with 3% sodium hypochlorite. Group II samples were irrigated with ozonated water. Group III samples were irrigated firstly by distilled water followed by laser irradiation. Group IV samples were irrigated by ozonated water followed by laser irradiation. After the treatment, the teeth were kept in vials containing 2 mL of nutrient broth. The vials were then incubated at 37°C for 24 h. Standard methods were then used to identify grown colonies. Statistical analysis: Kruskal–Wallis test, Mann–Whitney test, Chi-square test, and Wilcoxon signed-rank test were used to measure the colony-forming units (CFUs) obtained at the end of the incubation period among the various groups for the evaluation of antibacterial efficacy of various disinfection protocols. Results: The highest mean CFU/mL is recorded in group II (with ozonated water) followed by group I (with sodium hypochlorite), group III (with a laser), and the least mean CFU/mL is seen in group IV (with laser and ozonated water). The variation in CFU/mL among the three groups is statistically significant (p < 0.05). Conclusion: The outcome of this study reveals that 980 nm diode laser along with ozonated water when used together can eliminate and disinfect the root canals with E. faecalis.


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  1. Weine FS. Endodontic Therapy. 3rd ed., St. Louis, London: The C.V. Mosby Company; 1982.
  2. Love RM. Invasion of dentinal tubules by root canal bacteria. Endod Topics 2004;9(1):52–65. DOI: 10.1111/j.1601-1546.2004.00078.x.
  3. Mohamed HE, Adel ME, Ahmed AZ. Electron microscopic study on the effect of diode laser and some irrigants on root canal dentinal wall. CDJ 2008;24(3):421–427.
  4. Charles HS, Scott A, Thomas J, et al. Enterococcus faecalis: it's role in root canal treatment failure and current concepts in retreatment. J Endod 2006;32(2):93–98. DOI: 10.1016/j.joen.2005.10.049.
  5. Siqueira J, Machado A, Silveira R, et al. Evaluation of the effectiveness of sodium hypochlorite used with three irrigation methods in the elimination of enterococcus faecalis from the root canal, in vitro. Int Endod J 1997;30(4):279–282. DOI: 10.1111/j.1365-2591.1997. tb00708.x.
  6. El-Batanouny M, EI-Khodery A, Zaky A. Electron microscopic study on the effect of diode laser and some irrigants on root canal dentinal walls. Cairo Dent J 2008;24(3):421–427.
  7. Sunnen G. Ozone in medicine: Overview and future directions. J Adv Med 1988;1(3):159–174.
  8. Komali G. Ozone therapy: a revolutionary non invasive therapy in dentistry. Open Access Sci Rep 2012;1(12):1–4.
  9. Hems RS, Gulabivala K, Ready D, et al. An in vitro evaluation of the ability of ozone to kill a strain of Enterococcus faecalis. Int Endod J 2005;38(1):22–29. DOI: 10.1111/j.1365-2591.2004.00891.x.
  10. Broadwater WT, Coehn RC, King PH. Sensitivity of three selected bacterial species to ozone. Appl Microbiol 1973;26(3):391–393. DOI: 10.1128/AM.26.3.391-393.1973.
  11. Kouchi Y, Ninomiya J, Yasuda H, et al. Location of Streptococcus mutans in the dentinal tubules of open infected root canals. J Dent Res 1980;59(12):2038–2046. DOI: 10.1177/00220345800590120301.
  12. Berutti E, Marini R, Angeretti A. Penetration ability of different irrigants into dentinal tubules. J Endod 1997;23(12):725–727. DOI: 10.1016/S0099-2399(97)80342-1.
  13. Portenier I, Waltimo TMT, Haapasalo M. Enterococcus faecalis-the root canal survivor and star in post-treatment disease. Endodontic Topics 2003;6(1):135–159. DOI: 10.1111/j.1601-1546.2003.00040.x.
  14. Lewis PR. Sodium hypochlorite root canal therapy. J Florida Dent Soc 1954;24(1):10–11.
  15. Estrela C, Estrela CRA, Barbin EL, et al. Mechanism of action of sodium hypochlorite. Braz Dent J 2002;13(2):113–117. DOI: 10.1590/S0103-64402002000200007.
  16. Farren ST, Sadoff RS, Penna KJ. Sodium hypochlorite chemical burn: case report. NYSDJ 2008;74(1):61–62.
  17. Eldeniz AU, Ozer F, Hadimli HH, et al. Bactericidal efficacy of ErCr:YSGG laser irradiation against Enterococcus faecalis compared with sodium hypochlorite irrigation: an ex vivo pilot study. Int Endod J 2007;40(2):112–119. DOI: 10.1111/j.1365-2591.2006.01190.x.
  18. Mithra N, Krishna R, Shishir S, et al. Comparative evaluation of bactericidal effects on Enterococcus faecalis using diode laser irradiation, sodium hypochlorite and chlorhexidine gluconate irrigation”- an in vitro study. Oral Health Dent Manag 2013;12(3):145–150.
  19. Brazzelli M, McKenzie L, Fielding S, et al. Systematic review of the effectiveness and cost-effectiveness of HealOzone for the treatment of occlusal pit/fissure caries and root caries. Health Techno Assess 2006;10(16):3–4. DOI: 10.3310/hta10160.
  20. Loncar B, Mravak Stipetic M, Matosevic D, et al. Ozone application in dentistry. Arch Med Res 2009;40(2):136–137. DOI: 10.1016/j.arcmed.2008.11.002.
  21. Nagayoshi M, Kitamura C, Fukuizumi T, et al. Antimicrobial effect of ozonated water on bacteria invading dentinal tubules. J Endod 2004;30(11):778–781. DOI: 10.1097/00004770-200411000-00007.
  22. Klinke T, Klimm W, Gutknecht N. Antibacterial effects of Nd:YAG laser irradiation within root canal dentin. J Clin Laser Med Surg 1997;15(1):29–31. DOI: 10.1089/clm.1997.15.29.
  23. Kreisler M, Kohnen W, Beck M, et al. Efficacy of NaOCI/H2O2 irrigation and GaAlAs laser in decontamination of root canals in vitro. Lasers Surg Med 2003;32(3):189–196. DOI: 10.1002/lsm.10148.
  24. Hardee MW, Miserendino L, Kos W, et al. Evaluation of the antibacterial effects of intracanal Nd: YAG laser irradiation. J Endod 1994;20(8):377–380. DOI: 10.1016/S0099-2399(06)80294-3.
  25. Myers TD, McDaniel JD. The pulsed Nd: YAG laser: review of clinical applications. J Calif Dent Assoc 1991;19(11):25–30.
  26. Moritz N, Gutknecht K, Goharkhay K, et al. In vitro irradiation of infected root canals with a diode laser: results of microbiological, infrared spectrometric, and stain penetration examinations. Quintessence Int 1997;28(3):205–209.
  27. Schoop U, Kluger W, Moritz A, et al. Bactericidal effect of different laser systems in the deep layers of dentin. Lasers Surg Med 2004;35(2):111–116. DOI: 10.1002/lsm.20026.
  28. Radaelli CM, Zezell DM, Cai S, et al. Effect of a high power diode laser irradiation in root canals contaminated with Enterococcus faecalis. “In vitro study”. Int Congr Scr 2003;1248:273–276. DOI: 10.1016/S0531-5131(02)01308-0.
  29. Souza LC, Brito PR, Oliveira JC, et al. Photodynamic therapy with two different photosensitizers as a supplement to instrumentation/irrigation procedures in promoting intracanal reduction of Enterococcus faecalis. J Endod 2010;36(2):292–296. DOI: 10.1016/j.joen.2009.09.041.
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