International Journal of Clinical Pediatric Dentistry

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VOLUME 13 , ISSUE 1 ( January-February, 2020 ) > List of Articles

Original Article

Impact of Calcium Glycerophosphate-supplemented Carbonated Beverages in Reducing Mineral Loss from the Enamel Surface

Y Hasita Manaswini

Keywords : Calcium glycerophosphate, Carbonated beverage, Erosion, Hardness, Tooth demineralization

Citation Information : Manaswini YH. Impact of Calcium Glycerophosphate-supplemented Carbonated Beverages in Reducing Mineral Loss from the Enamel Surface. Int J Clin Pediatr Dent 2020; 13 (1):1-5.

DOI: 10.5005/jp-journals-10005-1705

License: CC BY-NC 4.0

Published Online: 25-10-2015

Copyright Statement:  Copyright © 2020; The Author(s).


Aim: The aim of this study was to evaluate the surface microhardness and mineral loss from enamel exposed to carbonated beverages supplemented with and without calcium glycerophosphate (CaGP). Materials and methods: Forty enamel blocks were prepared from 20 extracted premolars, and their initial surface microhardness was measured using the Knoop microhardness testing machine. The samples were divided into four groups based on the concentration of CaGP added to the beverage: group I: beverage without CaGP (control group), group II: beverage with 2 mM CaGP, group III: beverage with 5 mM CaGP, and group IV: beverage with 10 mM CaGP. The samples were subjected to four cycles of exposure to plain and CaGP-supplemented carbonated beverage with an intermittent buffering in artificial saliva, after which the final surface microhardness was measured. The mineral loss from enamel blocks was estimated spectrophotometrically. Results: The obtained data were analyzed using paired t test and analysis of variance. A highly significant (p < 0.01) reduction in surface microhardness was observed in group I (beverage without CaGP). The reduction in surface microhardness in group III (beverage + 5 mM CaGP) and group IV (beverage + 10 mM CaGP) was not significantly different from that of sound enamel. A highly significant difference in calcium loss was observed between the groups (p = 0.00). Calcium loss reduced as the CaGP concentration increased in the groups. A similar trend was observed when phosphate loss was analyzed. Conclusion: Addition of CaGP to the carbonated beverages significantly prevented the reduction in surface microhardness of enamel and mineral loss. As the concentration of CaGP in carbonated beverages increased from 2 mM to 10 mM, the mineral loss is decreased. Clinical significance: Consumption of carbonated beverages has been increasing among the children and adolescents, leading to a higher incidence of dental erosion and caries. Hence, supplementation of these acidic beverages with buffering agents such as CaGP may help in preventing such dental problems among vulnerable populations.

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  1. Tahmassebi J, Duggal M, Malik-Kotru G, et al. Soft drinks and dental health: a review of the current literature. J Dent 2006;34(1):2–11. DOI: 10.1016/j.jdent.2004.11.006.
  2. Attin T, Weiss K, Becker K, et al. Impact of modified acidic soft drinks on enamel erosion. Oral Dis 2005;11(1):7–12. DOI: 10.1111/j.1601-0825.2004.01056.x.
  3. Kato M, Buzalaf M. Iron supplementation reduces the erosive potential of a cola drink on enamel and dentin in situ. J Appl Oral Sci 2012;20(3):318–322. DOI: 10.1590/S1678-77572012000300004.
  4. Lynch RJ. Calcium glycerophosphate and caries: a review of the literature. Int Dent J 2004;54(5 Suppl 1):310–314. DOI: 10.1111/j.1875-595X.2004.tb00004.x.
  5. Barbosa C, Montagnolli L, Kato M, et al. Calcium glycerophosphate supplemented to soft drinks reduces bovine enamel erosion. J Appl Oral Sci 2012;20(4):410–413. DOI: 10.1590/S1678-77572012000400004.
  6. Kato MT, Italiani FM, Araujo JJ, et al. Preventive effect of an iron varnish on bovine enamel erosion in vitro. J Dent 2009;37(3):233–236. DOI: 10.1016/j.jdent.2008.11.019.
  7. Sato Y, Sato T, Niwa M, et al. Precipitation of octacalcium phosphates on artificial enamel in artificial saliva. J Mater Sci Mater Med 2006;17(11):1173–1177. DOI: 10.1007/s10856-006-0545-4.
  8. Bhaumik S. The public health threat from sugary drinks in India. BMJ 2014;349:g6216. DOI: 10.1136/bmj.g6216.
  9. Sardana V, Balappanavar AY, Patil GB, et al. Impact of a modified carbonated beverage on human dental plaque and salivary pH: an in vivo study. J Indian Soc Pedod Prev Dent 2012;30(1):7–12. DOI: 10.4103/0970-4388.95563.
  10. Lussi A, Schaffner M. Progression of and risk factors for dental erosion and wedge-shaped defects over a 6-year period. Caries Res 2000;34(2):182–187. DOI: 10.1159/000016587.
  11. Mainwaring PJ, Naylor MN. A four-year clinical study to determine the caries-inhibiting effect of calcium glycerophosphate and sodium fluoride in calcium carbonate base dentifrices containing sodium monofluorophosphate. Caries Res 1983;17(3):267–276. DOI: 10.1159/000260677.
  12. Grenby TH. Trials of three organic phosphorus-containing compounds as protective agents against dental caries in rats. J Dent Res 1973;52(3):454–461. DOI: 10.1177/00220345730520031201.
  13. Grenby TH, Bull JM. Protection against dental caries in rats by glycerophosphates or calcium salts or a mixture of both. Arch Oral Biol 1975;20(11):717–724. DOI: 10.1016/0003-9969(75)90041-2.
  14. Grenby TH, Bull JM. Dental caries in laboratory rats from breakfast cereals and its control by calcium glycerophosphate additive. Arch Oral Biol 1978;23(8):675–680. DOI: 10.1016/0003-9969(78)90193-0.
  15. Zaze A, Dias A, Amaral J, et al. In situ evaluation of low-fluoride toothpastes associated to calcium glycerophosphate on enamel remineralization. J Dent 2014;42(12):1621–1625. DOI: 10.1016/j.jdent.2014.09.001.
  16. Code of Federal Regulations [Title 21, Volume 3] [Revised as of April 1, 2018] [CITE: 21CFR184.1201].
  17. Schlueter N, Hara A, Shellis R, et al. Methods for the measurement and characterization of erosion in enamel and dentine. Caries Res 2011;45(Suppl 1):13–23. DOI: 10.1159/000326819.
  18. Ten Cate JM, Larsen MJ, Pearce EIF, et al. Chemical interactions between the tooth and oral fluids. In: Fejerskov O, Kidd EAM, ed. Dental Caries: The Disease and its Clinical Management. Oxford: Blackwell Publishing; 2003. pp. 49–69.
  19. Magalhaes AC, Moraes SM, Rios D, et al. Effect of ion supplementation of a commercial soft drink on tooth enamel erosion. Food Addit Contam Part A Chem Anal Control Expo Risk Assess 2009;26(2):152–156. DOI: 10.1080/02652030802425326.
  20. Grenby T, Bull J. Chemical studies of the protective action of phosphate compounds against the demineralization of human dental enamel in vitro. Caries Res 1980;14(4):210–220. DOI: 10.1159/000260456.
  21. Gnass C, Klimek J, Schwarz N. A comparative profilometric in vitro study of the susceptibility of polished and natural human enamel and dentin surfaces to erosive demineralization. Arch Oral Biol 2000;45(10):897–902. DOI: 10.1016/S0003-9969(00)00041-8.
  22. Tenovuo J, Rekola M. Some effects of sugar-flavored acid beverages on the biochemistry of human whole saliva and dental plaque. Acta Odontol Scand 1977;35(6):317–330. DOI: 10.3109/00016357709064131.
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