International Journal of Clinical Pediatric Dentistry

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VOLUME 12 , ISSUE 5 ( September-October, 2019 ) > List of Articles

Original Article

Comparative Analysis of CPP–ACP, Tricalcium Phosphate, and Hydroxyapatite on Assessment of Dentinal Tubule Occlusion on Primary Enamel Using SEM: An In Vitro Study

Meghna Bajaj

Keywords : CPP–ACP, Dentinal hypersensitivity, Hydroxyapatite, Scanning electron microscope, Tricalcium phosphate

Citation Information : Bajaj M. Comparative Analysis of CPP–ACP, Tricalcium Phosphate, and Hydroxyapatite on Assessment of Dentinal Tubule Occlusion on Primary Enamel Using SEM: An In Vitro Study. Int J Clin Pediatr Dent 2019; 12 (5):371-374.

DOI: 10.5005/jp-journals-10005-1680

License: CC BY-NC 4.0

Published Online: 01-02-2020

Copyright Statement:  Copyright © 2019; The Author(s).


Background: Over the last few decades, fluoride in various forms has been proved to reduce caries and dentinal hypersensitivity in both the primary and permanent dentitions. Recently, newer materials containing calcium and phosphate ions, tricalcium phosphate, and hydroxyapatite has received much attention. Aim: The aim of the study was to compare CPP–ACP, tricalcium phosphate, and hydroxyapatite in relation to the assessment of dentine tubule occlusion on primary enamel using scanning electron microscope (SEM). Materials and methods: Forty freshly extracted noncarious primary molars were randomly divided into 4 groups (I–IV) with 10 sections in each group—group I: negative control, group II: CPP–ACP, group III: tricalcium phosphate, group IV: hydroxyapatite. To assess tubule occlusion, twenty dentin sections of 2-mm thickness were obtained from the cervical third of sound primary molars. Each section was processed to simulate the hypersensitive dentin and the test agents were brushed over the sections with an electric toothbrush and observed under a SEM for calculation of the percentage of occluded tubules. Results: Groups II and IV showed a greater percentage of tubule occlusion than group III. An intergroup comparison of tubule occlusion potential of groups II and IV was not significant. Conclusion: Hydroxyapatite showed significantly higher dentinal tubule occlusion when compared to CPP–ACP and tricalcium phosphate.

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  1. Parolia A, Kundabala M, et al. Management of dentinal hypersensitivity: a review. J Calif Dent Assoc 2011;39:167–179.
  2. Addy M, Hunter ML. Can tooth brushing damage your health? Effects on oral and dental tissues. Int Dent J 2003;53(3):177–186.
  3. Splieth CH, Tachou A. Epidemiology of dentin hypersensitivity. Clin Oral Invest 2013;17(1):S3–S8. DOI: 10.1007/s00784-012-0889-8.
  4. Cunha-Cruz J, Wataha JC, et al. Northwest Practice-based Research Collaborative in Evidence-based dentistry. The prevalence of dentin hypersensitivity in general dental practices in the northwest United States. J Am Dent Assoc 2013;144:288–296. DOI: 10.14219/jada.archive.2013.0116.
  5. Taji S, Seow WK. A literature review of dental erosion in children. Australian Dental Journal 2010;55:358–367. DOI: 10.1111/j.1834-7819.2010.01255.x.
  6. Çolak H, Dülgergil CT, et al. Early childhood caries update: a review of causes, diagnoses, and treatments. J Nat Sci Biol Med 2013 Jan–Jun;4(1):29–38. DOI: 10.4103/0976-9668.107257.
  7. Schmidlin PR, Sahrmann P. Current management of dentin hypersensitivity. Clin Oral Invest 2013;17(1):S55–S59. DOI: 10.1007/s00784-012-0912-0.
  8. Cummins D. Recent advances in dentin hypersensitivity: clinically proven treatments for instant and lasting sensitivity relief. Am J Dent 2010;23(Spec No A):3A–13A.
  9. Gendreau L, Barlow AP, et al. Overview of the clinical evidence for the use of NovaMin in providing relief from the pain of dentin hypersensitivity. J Clin Dent 2011;22:90–95.
  10. Ritter AV, de La Dias W, et al. Treating cervical dentin hypersensitivity with fluoride varnish: a randomized clinical study. J Am Dent Assoc 2006;137:1013–1020. DOI: 10.14219/jada.archive.2006.0324.
  11. Reynolds EC. Remineralization of enamel subsurface lesions by casein phosphopeptide-stabilized calcium phosphate solutions. J Dent Res 1997;76(9):1587–1595. DOI: 10.1177/00220345970760091101.
  12. Balakrishnan A, Jonathan R, et al. Evaluation to determine the caries remineralization potential of three dentifrices: An in vitro study. J Cons Dent 2013;16(4):375–379. DOI: 10.4103/0972-0707.114347.
  13. Benjamin S, Roshni, et al. Seal that heals. World J Dent 2012;3:243–246. DOI: 10.5005/jp-journals-10015-1164.
  14. Prabhakar AR, Manojkumar AJ, et al. In vitro remineralization of enamel subsurface lesions and assessment of dentine tubule occlusion from NaF dentifrices with and without calcium. J Indian Soc Pedod Prev Dent 2013;31(1):29–35. DOI: 10.4103/0970-4388.112403.
  15. Arrais GC, Micheloni CD, et al. Occluding effect of dentifrices on dentinal tubules. J Dent 2003;31:577–584. DOI: 10.1016/S0300-5712(03)00115-5.
  16. Gillam DG, Mordan NJ, et al. The effects of oxalate-containing products on the exposed dentine surface: an SEM investigation. J Oral Rehabil 2001;28:1037–1044. DOI: 10.1046/j.1365-2842.2001.00775.x.
  17. Kolker JL, Vargas MA, et al. Effect of desensitizing agents on dentin permeability and dentin tubule occlusion. J Adhes Dent 2002;4: 211–221.
  18. Yuan P, Shen X, et al. Effects of dentifrice containing hydroxyapatite on dentinal tubule occlusion and aqueous hexavalent chromium cations sorption: a preliminary study. PLoS One 2012;7(12):e45283. DOI: 10.1371/journal.pone.0045283.
  19. Garberoglio R, Brannstrom M. Scanning electron microscope investigation of human dentinal tubules. Arch Oral Biol 1976;21: 355–362. DOI: 10.1016/S0003-9969(76)80003-9.
  20. Hirayama A, Yamada M, et al. Analytical electron microscopic studies on the dentinal tubules of human deciduous teeth. J Dent Res 1985;64:743.
  21. West NX, Hughes JA, et al. The effect of pH on erosion of dentine and enamel by dietary acids in vitro. J oral Rehabil 2001;28:860–864. DOI: 10.1111/j.1365-2842.2001.00778.x.
  22. Pashley EL, Talman R, et al. Permeability of normal versus carious dentin. Endod Dent Traumatol 1991;7:207–211. DOI: 10.1111/j.1600-9657.1991.tb00437.x.
  23. Tagami J, Hosoda H, et al. Effect of aging and caries on dentin permeability. Proc Finn Dent Soc 1992;88(Suppl. 1):149–154.
  24. Pashley DH. Dentin-predentin complex and its permeability: physiologic overview. J Dent Res 1985;64(Special Issue):613–620. DOI: 10.1177/002203458506400419.
  25. Kowalczyk A, Botulinski B, et al. Evaluation of the product based on RecaldentTM technology in the treatment of dentin hypersensitivity. Adv Med Sci 2006;51:40–42.
  26. Shen P, Manton DJ, et al. Effect of added calcium phosphate on enamel remineralization by fluoride in a randomized controlled in situ trial. J Dent 2011;39:518–525. DOI: 10.1016/j.jdent.2011. 05.002.
  27. Mackey AC. In Vitro Assessment of Dentin Tubule Occlusion by Hypersensitivity Dentifrices. 2009 April 1–4; available from: 120922. Html.
  28. Kamath U, Sheth H, et al. The effect of Remin Pro on bleached enamel hardness: An in vitro study. Indian J Dent Res 2013;24(6):690–693. DOI: 10.4103/0970-9290.127612.
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