International Journal of Clinical Pediatric Dentistry

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VOLUME 12 , ISSUE 2 ( March-April, 2019 ) > List of Articles

Original Article

Effect of Green Tea Extract Mouthwash on Salivary Streptococcus mutans Counts in a Group of Preschool Children: An In vivo Study

Mohamed Th Salama, Zeyad A Alsughier

Keywords : Catechin, Green tea, Mouthwash, Preschool children, Randomized controlled clinical trial, Streptococcus mutans

Citation Information : Salama MT, Alsughier ZA. Effect of Green Tea Extract Mouthwash on Salivary Streptococcus mutans Counts in a Group of Preschool Children: An In vivo Study. Int J Clin Pediatr Dent 2019; 12 (2):133-138.

DOI: 10.5005/jp-journals-10005-1610

License: CC BY-NC 4.0

Published Online: 01-09-2019

Copyright Statement:  Copyright © 2019; The Author(s).


Abstract

Aim: This study was conducted to evaluate the effectiveness of green tea mouthwash on the salivary level of Streptococcus mutans in the preschool children. Materials and methods: In this randomized controlled clinical trial, 40 cooperative children (4-5 years old) were divided into two groups. The study group included 20 children who did the routine tooth brushing 3 times/day, and then green tea extract mouthwash (8 mL/day) 2 times/day for 4 weeks. The control group included other 20 children who did the routine tooth brushing as the study group but did not use any green tea extract mouthwash. The quantitative microbiological laboratory cultivation method of S. mutans was carried out for each child at the baseline, after 2 weeks, and after 4 weeks of the study period. Results: Statistically, the results showed that there was a statistically significant difference in the mean log S. mutans counts between the study and control groups in both follow-up periods after 2 weeks and after 4 weeks. Also, there were statistically significant mean percentage decreases in log S. mutans counts for the two groups. Conclusion: The use of green tea mouthwash showed promising results in reducing the cariogenic salivary S. mutans counts. Clinical significance: Green tea extract mouthwash is a nontoxic and safe, particularly for children. Catechins, the main bioactive ingredient of green tea, show an antibacterial action; thus, it has a promising effect in decreasing the count of salivary S. mutans and in the prevention of dental caries.

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  1. Zafar S, Harnekar SY, et al. Early childhood caries: etiology, clinical considerations, consequences and management. Int Dent Saudi Arabia 2009;11(4):24–36.
  2. Gussy MG, Waters EG, et al. Early childhood caries: current evidence for aetiology and prevention. J Paediatr Child Health 2006 Jan– Feb;42(1–2):37–43. DOI: 10.1111/j.1440-1754.2006.00777.x.
  3. Kwan SYL, Petersen PE, et al. Health-promoting schools: An opportunity for oral health promotion. Bull world Health Organ 2005;83(9):677–685. DOI: /S0042-96862005000900013.
  4. Motegi M, Takagi Y, et al. Assessment of genes associated with Streptococcus mutans biofilm morphology. Appl Environ Microbiol 2006;72(9):6277–6287. DOI: 10.1128/AEM.00614-06.
  5. Ahn SJ, Ahn SJ, et al. Characteristics of biofilm formation by Streptococcus mutans in the presence of saliva. Infect Immun 2008;76(9):4259–4268. DOI: 10.1128/IAI.00422-08.
  6. Banas JA. Virulence properties of Streptococcus mutans. Front Biosci 2004;9:1267–1277. DOI: 10.2741/1305.
  7. Perala SR, Bhupathiraju P. Efficacy of four fluoride mouth rinses on Streptococcus mutans in high caries risk children—a randomized controlled trial. J Clin Diagn Res 2016;10(9):ZC56–ZC60. DOI: 10.7860/JCDR/2016/16107.8508.
  8. Sundas S, Rao A. Comparative evaluation of chlorhexidine and sodium fluoride mouthwashes on Streptococcus mutans. J Nepal Dent Assoc 2011;12:17–21.
  9. Sham B, Sundeep H, et al. Antibacterial activity of green tea extract against Streptococcus mutans —in vitro study. J Indian Dent Assoc 2013;7:28–31.
  10. Moghbel A, Farizadeh A, et al. The effect of green tea on prevention of mouth bacterial infection, halitosis and plaque formation on teeth. Iran J Toxicol 2011;5:502–515.
  11. Sarin S, Marya C, et al. Preliminary clinical evidence of the antiplaque, antigingivitis efficacy of a mouthwash containing 2% green tea—a randomised clinical trial. Oral Health Prev Dent 2015;13:197–203. DOI: 10.3290/j.ohpd.a33447.
  12. Hamilton-Miller JM. Anti-cariogenic properties of tea (Camellia sinensis). J Med Microbiol 2001;50:299–302. DOI: 10.1099/0022-1317- 50-4-299.
  13. Becker R, Hirsh S, et al. Inhibitory Effects of Camellia sinensis (Green Tea) on Streptococcus mutans. Available from: http://www. depts.drew.edu/govschl/njgss2009/journal/TeamPapers/team3. pdf.
  14. Moghbel A, Farjzadeh A, et al. The effect of green tea on prevention of mouth bacterial infection, halitosis, and plaque formation on teeth. Iran J Toxicol 2011;5(14):504–506.
  15. Le Bell Y, Söderling E, et al. Effect of repeated sampling and prestimulation on saliva buffer capacity and flow rate values in children. Scand J Dent Res 1991;99:505–509.
  16. Wu-Yuan C, Chen C, et al. Gallochins inhibit growth, water insoluble glucan synthesis and aggregation of SM bacteria. J Dent Res 1988;67:51–55. DOI: 10.1177/00220345880670011001.
  17. Tehrani MH, Asghari G, et al. Comparing Streptococcus mutans and Lactobacillus colony count changes following green tea mouth rinse or sodium fluoride mouth rinse use in children (randomized doubleblind controlled clinical trial). Dent Res J (Isfahan) 2011;8(Suppl 1): S58–S63.
  18. Neturi RS, Srinivas R, et al. Effects of green tea on Streptococcus mutans counts—a randomised control trail. J Clin Diagn Res 2014;8:ZC128– ZC130. DOI: 10.7860/JCDR/2014/10963.5211.
  19. Curnow MM, Pine CM, et al. A randomised controlled trial of the efficacy of supervised toothbrushing in high-caries-risk children. Caries Res 2002;36:294–300. DOI: 10.1159/000063925.
  20. Ferrazzano GF, Roberto L, et al. Antimicrobial properties of green tea extract against cariogenic microflora: an in vivo study. J Med Food 2011;14:907–911. DOI: 10.1089/jmf.2010.0196.
  21. Hashimoto F, Ono M, et al. Evaluation of the anti-oxidative effect (in vitro) of tea polyphenols. Biosci Biotechnol Biochem 2003;67:396–401. DOI: 10.1271/bbb.67.396.
  22. Yang CS, Wang X, et al. Cancer prevention by tea: animal studies, molecular mechanisms and human relevance. Nat Rev Cancer 2009;9:429–439. DOI: 10.1038/nrc2641.
  23. Awadalla HI, Ragab MH, et al. A pilot study of the role of green tea use on oral health. Int J Dent Hyg 2011;9:110–116. DOI: 10.1111/j.1601- 5037.2009.00440.x.
  24. Matsumoto M, Minami T, et al. Inhibitory effects of oolong tea extract on caries-inducing properties of mutans streptococci. Caries Res 1999;33:441–445. DOI: 10.1159/000016549.
  25. Thomas A, Thakur SR, et al. Anti-microbial efficacy of green tea and chlorhexidine mouth rinses against Streptococcus mutans, Lactobacilli spp. and Candida albicans in children with severe early childhood caries: a randomized clinical study. J Indian Soc Pedod Prev Dent 2016;34:65–70. DOI: 10.4103/0970-4388.175518.
  26. Goyal AK, Bhat M, et al. Effect of green tea mouth rinse on Streptococcus mutans in plaque and saliva in children: an in vivo study. J Indian Soc Pedod Prev Dent 2017;35:41–46. DOI: 10.4103/0970-4388.199227.
  27. Abdelmegid F, Al-Agamy M, et al. Effect of honey and green tea solutions on Streptococcus mutans. J Clin Pediatr Dent 2015;39:435–441. DOI: 10.17796/1053-4628-39.5.435.
  28. Hirao K, Yumoto H, et al. Tea catechins reduce inflammatory reactions via mitogen-activated protein kinase pathways in toll-like receptor 2 ligand-stimulated dental pulp cells. Life Sci 2010;86:654–660. DOI: 10.1016/j.lfs.2010.02.017.
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