Citation Information :
Thomas J, Bilichodmath S, Preethi N. Identification of Specific Anaerobic Bacteria in Endodontic Infections of Primary Teeth—A PCR Study. Int J Clin Pediatr Dent 2019; 12 (1):1-4.
Introduction: Invasion of microorganisms and their multiplication in root canals (RCs) results in endodontic infections of primary teeth. Acute and chronic inflammation may be present in the periapical area and are based on the amount and virulence of microorganisms, especially anaerobic bacteria present in the RC. To identify microorganisms very precisely in endodontic infections, polymerase chain reaction (PCR) is used.
Aim: The aim of the present study is to identify the specific anaerobic bacteria like Porphyromonas gingivalis, Prevotella intermedia, and Actinomyces naeslundii in the RCs of primary teeth using real-time PCR.
Methodology: Fifteen subjects aged 3-8 years who had endodontic infections in primary molars were selected. The cases who had been selected did not receive any endodontic treatment and antibiotics within 3 months, and children with systemic diseases were not included.
Sample collection: Samples were taken by placing absorbent paper points into the largest canals of maxillary and mandibular molars for 60 seconds and are then transferred to a sterile Eppendorf tube with tris-hydochloride EDTA (TE) buffer. The samples were stored at ?80°C. All samples were subjected to PCR analysis.
Result: The specific anaerobes detected in the samples were A. naeslundii (93.3%), Prevotella intermedia (53.3%), and Porphyromonas gingivalis (13.3%).
Conclusion: The results suggested a high bacterial diversity in the RCs of infected primary teeth.
Gomes GB, Onofre SR, et al. An investigation of the presence of specific anaerobic species in necrotic primary teeth. Braz Oral Res 2013;27(2):149–155. DOI: 10.1590/S1806-83242013000100020.
Cogulu D, Uzel A, et al. PCR-based identification of selected pathogens associated with endodontic infections in deciduous and permanent teeth. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2008;106:443–449. DOI: 10.1016/j.tripleo.2008.03.004.
Cogulu D, Uzel A, et al. Detection of Enterococcus faecalis in necrotic teeth root canals by culture and polymerase chain reaction methods. Eur J Dent 2007;1:216–221.
Jacinto RC, Montagner F, et al. Frequency, microbial interactions, and antimicrobial susceptibility of Fusobacterium nucleatum and Fusobacterium necrophorum isolated from primary endodontic infections. J Endod 2008;34(12):1451–1456. DOI: 10.1016/j.joen.2008.08.036.
Tavares WLF, Brito LCN, et al. Microbiota of deciduous endodontic infections analyzed by MDA and Checkerboard DNA–DNA hybridization. Int Endod J 2011;44(3):225–235. DOI: 10.1111/j.1365-2591.2010.01805.x.
Yang QB, Fan LN, et al. Polymerase chain reaction–denaturing gradient gel electrophoresis, cloning, and sequence analysis of bacteria associated with acute periapical abscesses in children. J Endod 2010;36:218–223. DOI: 10.1016/j.joen.2009.11.001.
Cao H, Qi H, et al. Detection of Porphyromonas endodontalis, Porphyromonas gingivalis and Prevotella intermedia in primary endodontic infections in a Chinese population. Int Endod J 2012;45(8):773–781. DOI: 10.1111/j.1365-2591.2012.02035.x.
Topcuoglu N, Bozdoðan E, et al. Presence of oral bacterial species in primary endodontic infections of primary teeth. J Clin Pediatr Dent 2013;38(2):155–160.
Kutllovci T, Iljovska S, et al. Bacteriological identification of selected pathogens in infected primary and young permanent teeth associated with clinical symptoms. Open J Med Microbiol 2015;5:59–68.
Tang G, Samaranayake LP, et al. Direct detection of Actinomyces spp. from infected root canals in a Chinese population: a study using PCR-based, oligonucleotide–DNA hybridization technique. J Dent 2003;31:559–568. DOI: 10.1016/S0300-5712(03)00112-X.
Siqueira JF, Rocas IN. Diversity of endodontic microbiota revisited. J Dent Res 2009;88(11):969–981. DOI: 10.1177/0022034509346549.