International Journal of Clinical Pediatric Dentistry

Register      Login

SEARCH WITHIN CONTENT

FIND ARTICLE

Volume / Issue

Online First

Archive
Volume  17 (2024)
Volume  16 (2023)
Volume  15 (2022)
Volume  14 (2021)
Volume  13 (2020)
Volume  12 (2019)
Volume  11 (2018)
Volume  10 (2017)
Volume  9 (2016)
Volume  8 (2015)
Volume  7 (2014)
Volume  6 (2013)
Volume  5 (2012)
Volume  4 (2011)
Volume  3 (2010)
Volume  2 (2009)
Volume  1 (2008)
Related articles

VOLUME 6 , ISSUE 3 ( September-December, 2013 ) > List of Articles

RESEARCH ARTICLE

Comparison of Antibacterial Efficacy of Calcium Hydroxide Paste, 2% Chlorhexidine Gel and Turmeric Extract as an Intracanal Medicament and their Effect on Microhardness of Root Dentin: An in vitro Study

AR Prabhakar, Swapnil Taur, Savita Hadakar, S Sugandhan

Keywords : Intracanal medicaments, Ca(OH)2, 2% Chlorhexidine gel, Turmeric extract, Microhardness

Citation Information : Prabhakar A, Taur S, Hadakar S, Sugandhan S. Comparison of Antibacterial Efficacy of Calcium Hydroxide Paste, 2% Chlorhexidine Gel and Turmeric Extract as an Intracanal Medicament and their Effect on Microhardness of Root Dentin: An in vitro Study. Int J Clin Pediatr Dent 2013; 6 (3):171-177.

DOI: 10.5005/jp-journals-10005-1213

License: CC BY-NC 4.0

Published Online: 01-06-2019

Copyright Statement:  Copyright © 2013; The Author(s).


Abstract

Aim: To evaluate and compare the antibacterial efficacy of turmeric extract as an intracanal medicament against E. faecalis and its effect on the microhardness of root dentin in comparison with calcium hydroxide and 2% chlorhexidine gel. Materials and methods: One hundred and fourty dentin blocks were prepared from 70 extracted human single-rooted teeth and standardized. For antibacterial assessment, 120 blocks were infected for 21 days with E. faecalis (n = 24/group). Dentin blocks were treated with group I (Ca(OH)2), group II (2% chlorhexidine gel), group III (turmeric extract), group IV (saline) and group V (negative control). Dentin shavings were obtained in TSB at depth of 400 μm and plated to count CFUs at 24 hours, 3 and 7 days (n = 8/day). For microhardness assessment, eight samples of 2 mm thickness were prepared form four dentin blocks (n = 8/group). Following treatment with medicaments, microhardness test was performed at 24 hours, 3 and 7 days using Vickers hardness indentation machine at 400 μm from canal lumen. Results: Complete inhibition of E. faecalis was observed with group II, followed by 64% with group I and 54% with group III which was statistically highly significant (p < 0.001). Highest effect on microhardness of root dentin was shown by group I, followed by group II and no effect was seen with group III which was statistically highly significant (p < 0.001). Conclusion: Turmeric extract has substantial antibacterial activity with no effect on microhardness of root dentine and hence has a potential to be used as intracanal medicament if its antibacterial activity could be enhanced. How to cite this article: Prabhakar AR, Swapnil T, Savita H, Sugandhan S. Comparison of Antibacterial Efficacy of Calcium Hydroxide Paste, 2% Chlorhexidine Gel and Turmeric Extract as an Intracanal Medicament and their Effect on Microhardness of Root Dentin: An in vitro Study. Int J Clin Pediatr Dent 2013;6(2):171-177.

PDF Share
  1. Law A, Messer H. An evidence-based analysis of the antibacterial effectiveness of intracanal medicaments. J Endod 2004;30(10):689-694.
  2. Santhorn C, Parashos P, Messer H. Antibacterial efficacy of calcium hydroxide intracanal dressing: a systematic review and meta-analysis. Int Endod J 2007;40(1):2-10.
  3. Ferraz CC, Gomes BP, Zaia AA, Teixeira FB, Souza- Filho FJ. In vitro assessment of the antimicrobial action and mechanical ability or chlorhexidine gel as an endodontic irrigant. J Endod 2001;27(7):452-455.
  4. Groppo FC, Bergamaschi Cde C, Cogo K, Franz-Montan M, Motta R, de Andrade ED. Use of Phytotherapy in Dentistry. Phytother Res 2008;22(8):993-998.
  5. Chaturvedi TP. Uses of turmeric in dentistry in dentistry - an update. Indian J Dent Res 2009;20(1):107-109.
  6. Yoldas O, Dogan C, Seydaoglu G. The effect of two different calcium hydroxide combination on root dentin microhardness. Int Endod J 2004;37(12):828-831.
  7. Oliveira LD, Carvalho CA, Nunes W, Valera MC, Camargo CH, Jorge AO. Effects of chlorhexidine and sodium hypochlorite on the microhardness of root canal dentin. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2007;104(4):e125-e128.
  8. Cwikla SJ, Belanger M, Giguere S, Progulske-Fox A, Vertucci FJ. Dentinal tubule disinfection using three calcium hydroxide formulations. J Endod 2005;31(1):50-52.
  9. Niamsa N, Sittiwet C. Antimicrobial Activity of Curcuma longa Aqueous Extract. J Pharmacol Toxicol 2009;4(4):173-177.
  10. Wang YJ, Pan MH, Cheng AL, Lin LI, Ho YS, Hsieh CY, Lin JK. Stability of curcumin in buffer solutions and characterization of its degradation products. J Pharm Biomed Anal 1997;15(12):1867-1876.
  11. Krithikadatta J, Indira R, Dorothykalyani AL. Disinfection of dentinal tubules with 2% chlorhexidine, 2% metronidazole, bioactive glass when compared with calcium hydroxide as intracanal medicament. J Endod 2007;33(12):1473-1476.
  12. Porteneir J, Haapsolon, Ostrovik D, Yamauchi M, Haapasalo M. Inactivation of the antibacterial activity of iodine potassium iodide and chlorhexidinedigluconate against Enterococcus faecalis by dentin, dentin matrix, type-I collagen and heat killed microbial whole cell. J Endod 2002;28(9):634-637.
  13. Rai D, Singh JK, Roy N, Panda D. Curcumin inhibits FtsZ assembly: an attractive mechanism for its antibacterial activity. Biochem J 2008;410(1):147-155.
  14. White JD, Lacefield WR, Chavers LS, Eleaser PD. The effect of three commonly used endodontics material on the strength and hardness of root dentin. J Endod 2002;28(12):828-830.
  15. Saleh AA, Ettman WM. Effect of endodontic irrigation solutions on microhardness of root canal dentin. J Dent 1999;27(1):43-46.
PDF Share
PDF Share

© Jaypee Brothers Medical Publishers (P) LTD.