Introduction: Dental caries is an infectious disease with Streptococcus mutans as the main cariogenic bacteria. Children with early S. mutans colonization have a higher risk of developing dental caries than those with later colonization. Therefore, prevention or delay of S. mutans colonization may be advantageous for the prevention of early childhood caries (ECC). Aim: To evaluate and compare the effectiveness of xylitol and placebo wipes on S. mutans count in 19- to 35-monthold children. Materials and methods: Forty-four children were randomly selected from a daycare center and divided into two groups. Allocation concealment was done and both (placebo and xylitol) wipes were distributed to their parents. Instructions were given regarding their use, to be used twice daily for 2 weeks and the S. mutans levels in the saliva were enumerated before and after wipes usage. The collected data were tabulated and statistically analyzed using paired and unpaired t-tests. Results: A clinically significant decrease in the S. mutans count was observed in the xylitol wipes group than the placebo wipes group. Intergroup comparison results were found to be statistically insignificant. Conclusion: Xylitol wipes usage could serve as a useful adjunct in reducing the cariogenic bacteria, especially S. mutans, and thus can be considered as an adjunct oral hygiene tool for caries prevention in young children