International Journal of Clinical Pediatric Dentistry

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VOLUME 3 , ISSUE 3 ( September-December, 2010 ) > List of Articles

ORIGINAL ARTICLE

To Evaluate Cytotoxicity of Resin-based Restorative Materials on Human Lymphocytes by Trypan Blue Exclusion Test: An in vitro Study

Vinay Kumar Srivastava, Rajat Kumar Singh, SN Malhotra

Citation Information : Kumar Srivastava V, Kumar Singh R, Malhotra S. To Evaluate Cytotoxicity of Resin-based Restorative Materials on Human Lymphocytes by Trypan Blue Exclusion Test: An in vitro Study. Int J Clin Pediatr Dent 2010; 3 (3):147-152.

DOI: 10.5005/jp-journals-10005-1070

License: CC BY-NC 4.0

Published Online: 01-06-2018

Copyright Statement:  Copyright © 2010; The Author(s).


Abstract

The cytotoxicity of a resin-based material can be evaluated on isolated human lymphocyte. Since resin-based dental materials have been used with increasing frequency in anterior and posterior teeth restorations, the uncured resin monomers are leached out from the restorations and diffuses into the dentine and ultimately hampers the odontoblastic layers of pulp as well as gingiva. It is also reaches into the saliva and circulatory blood. The study evaluates and compares the relative cytotoxicity of resin-based dental materials at different time interval, i.e. 24, 48, and 72 hours on human lymphocyte by Trypan blue exclusion method. All resins were found to be cytotoxic to human lymphocyte. Resin samples cytotoxicity was the highest in first 24 hours followed by 48 and 72 hours.


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