International Journal of Clinical Pediatric Dentistry

Register      Login

SEARCH WITHIN CONTENT

FIND ARTICLE

Volume / Issue

Online First

Archive
Volume  17 (2024)
Volume  16 (2023)
Volume  15 (2022)
Volume  14 (2021)
Volume  13 (2020)
Volume  12 (2019)
Volume  11 (2018)
Volume  10 (2017)
Volume  9 (2016)
Volume  8 (2015)
Volume  7 (2014)
Volume  6 (2013)
Volume  5 (2012)
Volume  4 (2011)
Volume  3 (2010)
Volume  2 (2009)
Volume  1 (2008)
Related articles

VOLUME 9 , ISSUE 4 ( October-December, 2016 ) > List of Articles

Original Article

Blood Group Determination using DNA extracted from Exfoliated Primary Teeth at Various Time Durations and Temperatures: A PCR Study

Afreen Salman, Sundeep Hegde

Keywords : ABO, Blood grouping, Human identification, Polymerase chain reaction, Primary tooth, Pulp

Citation Information : Salman A, Hegde S. Blood Group Determination using DNA extracted from Exfoliated Primary Teeth at Various Time Durations and Temperatures: A PCR Study. Int J Clin Pediatr Dent 2016; 9 (4):308-312.

DOI: 10.5005/jp-journals-10005-1383

License: CC BY-NC 4.0

Published Online: 01-04-2019

Copyright Statement:  Copyright © 2016; The Author(s).


Abstract

Aim: To determine polymerase chain reaction (PCR)-based blood group on tooth pulp obtained from teeth stored for 1 month, 6 months, and 1 year following extraction and to evaluate the stability of deoxyribonucleic acid (DNA) in primary tooth subjected to a temperature of 200°C ± 5°C for 15 minutes. Materials and methods: Dental pulp tissue was collected from 40 exfoliated primary teeth stored for various time durations and temperature and preserved at 4°C till DNA extraction was carried out. Deoxyribonucleic acid was extracted using silica membrane-based spin-column procedure of QIAamp DNA minikit from BioRad. Deoxyribonucleic acid was subjected to PCR amplification and monoplex allele-specific PCR primers for ABO genotyping. Statistical analysis used: The data were analyzed by comparison (based on percentage). Results: In our study, overall, 85% samples showed a DNA yield. Cent percent results were obtained for samples studied at the end of 1 month followed by 90 and 80% for samples studied for 6 months and 1 year respectively. Heated samples showed 70% result. Conclusion: Polymerase chain reaction was found to be an effective method for blood group determination for teeth stored at various time durations and temperatures. However, as the time interval increased, the number of positive results obtained decreased. How to cite this article: Pai RK, Bhat SS, Salman A, Hegde S. Blood Group Determination using DNA extracted from Exfoliated Primary Teeth at Various Time Durations and Temperatures: A PCR Study. Int J Clin Pediatr Dent 2016;9(4):308-312.

PDF Share
  1. Rothwell BR. Principles of dental identification. Dent Clin North Am 2001 Apr;45(2):2253-2270.
  2. Woolridge ED Jr. Forensic dentistry. In: Eckert WG, editor. Introduction to forensic science. 2nd ed. St. Louis: C.V. Mosby Company; 1980. p. 114-117.
  3. Sweet D, Hilderand DP. Recovery of DNA from human teeth by cryogenic grinding. J Forensic Sci 1998 Oct;43(6): 1199-1202.
  4. Vij K. Identification. In: Textbook of forensic medicine and toxicology principles and practice. 3rd ed. New Delhi: Elsevier India; 2005. p. 50-51, 83.
  5. Pillay VV. Identification. In: Handbook of forensic medicine and toxicology. 14th ed. Hyderabad: Paras Medical Publishers, 2004. p. 48, 78.
  6. Shetty M, Premalatha K. ABO Blood Grouping from tooth material. J Indian Acad Forensic Med 32(4).
  7. Shetty HM, Shetty N, Karkera BV. Sex determination using dental tissue. Medicolegal Update 2008;8(2): 7-12.
  8. Sharma AK, Chattopadhyay PK. Blood groups and enzyme types from human teeth. J Forensic Sci Soc 1993 Jan- Mar;33(1):39-44.
  9. Kumar MG, Hegde AM. Sex identification from exfoliated primary teeth – a PCR study. J Clin Pediatr Dent 2005 Fall;30(1):19-22.
  10. Lee HY, Park MJ, Kim NY, Yang WI, Shin KJ. Rapid direct PCR for ABO Blood Typing. J Forensic Sci 2011 Jan;56(Suppl 1): 179-182.
  11. Xingzhi X, Ji L, Hao F, Ming L, Zhuyao L. ABO blood grouping on dental tissue. J Forensic Sci 1993;38(4):956-960.
  12. Inamdar P, Inamdar PK, Rai K, Mirajkar AM, Venkatesh S. Teeth – hidden treasure of blood group. Indian J Forensic Med Pathol 2011 Jul-Sep;4(3):113-118.
  13. Tsuchimochi T, Iwasa M, Maeno Y, Koyama H, Inoue H, Isobe I, Matoba R, Yokoi M, Nagao M. Chelating resin-based extraction of DNA from dental pulp and sex determination from incinerated teeth with y-chromosomal alphoid repeat and short tandem repeats. Am J Forensic Med Pathol 2002 Sep;23(3):268-271.
  14. Korszun AK, Causton BE, Lincoln PJ. Thermostability of ABO(H) blood-group antigens in human teeth. J Forensic Sci 1978 May-Jun;11(3):231-239.
PDF Share
PDF Share

© Jaypee Brothers Medical Publishers (P) LTD.