International Journal of Clinical Pediatric Dentistry

Register      Login

VOLUME 12 , ISSUE 6 ( November-December, 2019 ) > List of Articles

Original Article

Effect of Herbal Water, Ozonated Water, Water, and Chlorhexidine Mouthrinses on Oral Health Status of Children: A Randomized Controlled Trial

Jijo Mon, Sharath Asokan, Pollachi RG Priya, Thoppe DY Kumar, Mythili G Balasubramaniam

Citation Information : Mon J, Asokan S, Priya PR, Kumar TD, Balasubramaniam MG. Effect of Herbal Water, Ozonated Water, Water, and Chlorhexidine Mouthrinses on Oral Health Status of Children: A Randomized Controlled Trial. Int J Clin Pediatr Dent 2019; 12 (6):514-519.

DOI: 10.5005/jp-journals-10005-1693

License: CC BY-NC 4.0

Published Online: 24-11-2020

Copyright Statement:  Copyright © 2019; The Author(s).


Abstract

Aim: To evaluate the antibacterial effect of herbal water, ozonated water, and chlorhexidine mouthrinses on salivary Streptococcus mutans level and to assess their effectiveness of these mouthrinses on the oral health status of children. Materials and methods: A parallel multiarm randomized controlled trial was conducted in 100 children aged 10–12 years. Debris index-simplified (DI-S), calculus index-simplified (CI-S), oral hygiene index-simplified (OHI-S) scores, and Streptococcus mutans counts were recorded at baseline (T1). They were randomly divided into four equal groups (n = 25): HW, herbal water; OW, ozonated water; W, water; and CHX, chlorhexidine. Intervention period was 15 days and data collection was repeated after 15 days (T2) and 30 days (T3). Saliva samples were used to evaluate S. mutans count. Results were tabulated and analyzed statistically. Results: OW had minimum DI-S score at T2 and T3 periods. HW had the minimum CI-S score at T2 and T3 periods. CHX had the minimum S. mutans count at T2 and T3 periods. OW showed maximum reduction in OHI-S score at T2 period. HW showed maximum reduction in OHI-S score at T3 period. Conclusion: Herbal water and ozone water can be used as an alternative to chlorhexidine in maintaining the oral health status. Clinical significance: Herbal water and ozonated water can be used in children instead of chemical mouthrinses to avoid any adverse effects.


PDF Share
  1. Bagramian RA, Garcia-Godoy F, Volpe AR. The global increase in dental caries. A pending public health crisis. Am J Dent 2009;22(1): 3–8.
  2. Kaur RK, Singh MP, Chopra R, et al. To evaluate the efficacy of three commercially available herbal mouthwashes in treatment of chronic gingivitis: a comparative clinical study. Int J Dent Med Res 2014;1(4):42–46.
  3. Nagayoshi M, Fukuizumi T, Kitamura C, et al. Efficacy of ozone on survival and permeability of oral microorganisms. Oral Microbiol Immunol 2004;19(4):240–246. DOI: 10.1111/j.1399-302X.2004.00146.x.
  4. Deshmukh MA, Dodamani AS, Karibasappa G, et al. Comparative evaluation of the efficacy of probiotic, herbal and chlorhexidine mouthwash on gingival health: a randomized clinical trial. J Clin Diagn Res 2017;11(3):ZC13–ZC16. DOI: 10.7860/JCDR/2017/23891. 9462.
  5. Nagappan N, John J. Antimicrobial efficacy of herbal and CHX mouth rinse –a systematic review. J Dent Med Sci 2012;2(4):5–10.
  6. Ahmed SI, Sekhara Reddy VC, Sudhir KM, et al. Effect of tulsi extract and honey mouthrinses on salivary Streptococcus mutans count in comparison with 0.2% of chlorhexidine: a randomized controlled trial. J Indian Assoc Public Health Dent 2017;15:306–311. DOI: 10.4103/jiaphd.jiaphd_55_17.
  7. Mehta S, Pesapathy S, Joseph M, et al. Comparative evaluation of a herbal mouthwash (Freshol) with chlorhexidine on plaque accumulation, gingival inflammation, and salivary Streptococcus mutans growth. J Int Soc Prev Communit Dent 2013;3(1):25–28. DOI: 10.4103/2231-0762.115717.
  8. Agarwal P, Nagesh L. Comparative evaluation of efficacy of 0.2% chlorhexidine, listerine and tulsi extract mouth rinses on salivary Streptococcus mutans count of high school children-RCT. Contemp Clin Trials 2011;32(6):802–808. DOI: 10.1016/j.cct.2011.06.007.
  9. Hems RS, Gulabivala K, Ng YL, et al. An in vitro evaluation of the ability of ozone to kill a strain of Enterococcus faecalis. Int Endod J 2005;38(1):22–29. DOI: 10.1111/j.1365-2591.2004.00891.x.
  10. Arita M, Nagayoshi M, Fukuizumi T, et al. Microbicidal efficacy of ozonated water against Candida albicans adhering to acrylic denture plates. Oral Microbiol Immunol 2005;20(4):206–210. DOI: 10.1111/j.1399-302X.2005.00213.x.
  11. Baysan A, Lynch E. The use of ozone in dentistry and medicine. Part 2. Ozone and root caries. Prim Dent Care 2006;13(1):37–41. DOI: 10.1308/135576106775193897.
  12. Bezrukova IV, Petrukhina NB, Voinov PA. Experience in medical ozone use for root canal treatment. Stomatologiia (Mosk) 2005;84(6):20–22.
  13. Piaget J. Piaget and his school. Piaget's theory. Springer Berlin Heidelberg; 1976. pp. 11–23.
  14. WHO Oral Health Surveys - Basic Methods, 4th ed., New Delhi: A. I. T. B. S. Publishers and Distributors; 1997.
  15. Agarwal P, Nagesh L, Murlikrishnan. Evaluation of the antimicrobial activity of various concentrations of Tulsi (Ocimum sanctum) extract against Streptococcus mutans: an in vitro study. Indian J Dent Res 2010;21(3):357–359. DOI: 10.4103/0970-9290.70800.
  16. Anand SK, Ebenezar AV, Anand N, et al. A comparative analysis of antimicrobial property of wine and ozone with calcium hydroxide and chlorhexidine. J Clin Diagn Res 2015;9(6):ZC04–ZC06. DOI: 10.7860/JCDR/2015/11355.6030.
  17. Katti SS, Chava VK. Effect of ozonised water on chronic periodontitis - a clinical study. J Int Oral Health 2013;5(5):79–84.
  18. Dawes C, MacPherson LM. The distribution of saliva and sucrose around the mouth during the use of chewing gum and the implications for the site specificity of caries and calculus deposition. J Dent Res 1993;72(5):852–857. DOI: 10.1177/00220345930720050401.
  19. Löe H, Mandell M, Derry A, et al. The effect of mouthrinses and topical application of chlorhexidine on calculus formation in man. J Periodontal Res 1971;6(4):312–314. DOI: 10.1111/j.1600-0765.1971.tb00623.x.
  20. Löe H, Schiott CR. The effect of mouthrinses and topical application of chlorhexidine on the development of dental plaque and gingivitis in man. J Periodontal Res 1970;5:79–83. DOI: 10.1111/j.1600-0765.1970.tb00696.x.
  21. Celiberti P, Pazera P, Lussi A. The impact of ozone treatment on enamel physical properties. Am J Dent 2006;19:67–72.
  22. Anumula L, Kumar KVS, Krishna CM, et al. Antibacterial activity of freshly prepared ozonated water and chlorhexidine on mutans streptococcus when used as an oral rinse – a randomised clinical study. J Clin Diagn Res 2017;11(7):ZC05–ZC08.
  23. Cline NV, Layman DL. The effects of chlorhexidine on the attachment and growth of cultured human periodontal cells. J Periodontol 1992;63(7):598–602. DOI: 10.1902/jop.1992.63.7.598.
PDF Share
PDF Share

© Jaypee Brothers Medical Publishers (P) LTD.