International Journal of Clinical Pediatric Dentistry

Register      Login

VOLUME 3 , ISSUE 3 ( September-December, 2010 ) > List of Articles


To Evaluate Cytotoxicity of Resin-based Restorative Materials on Human Lymphocytes by Trypan Blue Exclusion Test: An in vitro Study

Vinay Kumar Srivastava, Rajat Kumar Singh, SN Malhotra

Citation Information : Kumar Srivastava V, Kumar Singh R, Malhotra S. To Evaluate Cytotoxicity of Resin-based Restorative Materials on Human Lymphocytes by Trypan Blue Exclusion Test: An in vitro Study. Int J Clin Pediatr Dent 2010; 3 (3):147-152.

DOI: 10.5005/jp-journals-10005-1070

License: CC BY-NC 4.0

Published Online: 01-06-2018

Copyright Statement:  Copyright © 2010; The Author(s).


The cytotoxicity of a resin-based material can be evaluated on isolated human lymphocyte. Since resin-based dental materials have been used with increasing frequency in anterior and posterior teeth restorations, the uncured resin monomers are leached out from the restorations and diffuses into the dentine and ultimately hampers the odontoblastic layers of pulp as well as gingiva. It is also reaches into the saliva and circulatory blood. The study evaluates and compares the relative cytotoxicity of resin-based dental materials at different time interval, i.e. 24, 48, and 72 hours on human lymphocyte by Trypan blue exclusion method. All resins were found to be cytotoxic to human lymphocyte. Resin samples cytotoxicity was the highest in first 24 hours followed by 48 and 72 hours.

PDF Share
  1. Sweeney M, Creanor SL, Smith RA, Foye RH. The release of mercury from dental amalgam and potential neurotoxicological effects. J Dent 2002 Jul-Aug;30(5-6):243-250
  2. Problems and benefits associated with restorative materials: side effects and long-term cost. Adv Dent Res 1992 Sep;6:7-16
  3. Cytotoxicity of a BIS-GMA dental composite before and after leaching in organic solvents. J Biomed Mater Res 1991 Apr;25(4):443-457
  4. Permeability of dentine. Malays J Med Sci 2003 Jan;10(1):27-36
  5. Cytotoxicity of composite materials polymerized with LED curing units. Oper Dent 2008 Jan-Feb;33(1):23-30
  6. Cytotoxicity of composite resins polymerized with different curing methods. Int Endod J 2004 Feb;37(2):151-156
  7. Genetic and cellular toxicology of dental resin monomers. J Dent Res 2006 Oct;85(10):870-877
  8. Diffusion of monomers from bonding resin-resin composite combinations through dentine in vitro. J Dent 1996 Jan-Mar;24(1-2):125-128.
PDF Share
PDF Share

© Jaypee Brothers Medical Publishers (P) LTD.