Citation Information :
Anandakrishna L, Chandra P, Nara A, D, D. Comparative Evaluation of Antimicrobial Efficacy of MTAD, 3% NaOCl and Propolis Against E Faecalis. Int J Clin Pediatr Dent 2010; 3 (1):21-25.
Aim : The present study sought to compare the antimicrobial efficacy of 3% NaOCl, Biopure MTAD (Tulsa Dentsply, Tulsa, OK) and Brazilian
ethanolic extract of propolis (EEP) against Enterococcus faecalis (E. faecalis).
Methodology : The study utilized 55 extracted human permanent teeth with single root canal. The samples were decoronated, instrumented
and sterilized. The teeth were infected with E faecalis for 48 hours. The teeth were divided randomly into 3 groups according to the
irrigants used and kept in contact with the respective irrigant for 5 minutes. All the samples were incubated in brain heart infusion (BHI)
broth for 96 hours. Disinfection of the samples was determined based on presence or absence of turbidity in the BHI broth 96 hours later.
Statistical analysis was done using Chi-square test.
Results : All the samples treated with MTAD showed complete absence of turbidity, while all the 15 teeth treated with propolis showed
presence of turbidity, 8 out of 15 teeth treated with NaOCl showed presence of turbidity. Statistical analysis of the data using chi-square
test showed significant difference between the groups (P < 0.05).
Conclusion : The study concluded that MTAD was more effective than 3% NaOCl and propolis against E. faecalis.
Kakehashi S, Stanley HR, Fitzerald RJ. The effects of surgical exposures of dental pulps in germ-free and conventional laboratory rats. Oral Surg Oral Med Oral Pathol 1965 Sep;20:340-349
Root canal obturation. J Am Dent Assoc 1956 Jul;53(1):47-55
Influence of infection at the time of root filling on the outcome of endodontic treatment of teeth with apical periodontitis. Int Endod J 1997 Sep;30(5):297-306
Association of Enterococcus faecalis with different forms of periradicular diseases. J Endod 2004 May;30(5):315-320
Disinfection by endodontic irrigants and dressings of experimentally infected dentinal tubules. Endod Dent Traumatol 1990 Aug;6(4):142-149
A bacteriological study of the pulp canals of infected primary molars. J Dent Child 1967 Nov;34(6):460- 470
Comparative study of the bacterial flora in the surroundings the root canals and sockets of deciduous molars. Int Dent J 1971 Sep;21(3):375-388. Comparative Evaluation of Antimicrobial Efficacy of MTAD, 3% NaOCl and Propolis Against E Faecalis International Journal of Clinical Pediatric Dentistry, January-April 2010;3(1):21-25 25 8. Byström A, Sundqvist G. Bacteriologic evaluation of the efficacy of mechanical root canal instrumentation in endodontic therapy. Scand J Dent Res 1981 Aug;89(4):321-328
The effect of sodium hypochlorite and chlorhexidine on cultured human periodontal ligament cells. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2001 Oct;92(4):446-450
A new solution for the removal of the smear layer. J Endod 2003 Mar;29(3):170-175
The antimicrobial effect of MTAD: an in vitro investigation. J Endod 2003 Jun;29(6):400-403
Histological analysis of rat dental pulp tissue capped with propolis. J Oral Sci 2005 Sep;47(3):135-138
A quantitative analysis of Propolis: A promising new storage media following avulsion. Dent Traumatol 2004 Apr;20(2):85-89
The influence of novel propolis on mutans streptococci biofilms and caries development in rats. Arch Oral Biol 2006 Jan;51(1):15-22
The effect of propolis on dentinal hypersensitivity and level of satisfaction among patients from a university hospital Riyadh, Saudi Arabia. Indian J Dent Res 1999 Oct-Dec;10(4):130-137
In vitro antimicrobial efficacy of MTAD and sodium hypochlorite. J Endod 2003 Jul;29(7):450-452
Mechanical reduction of the bacterial population in the root canal by three instrumentation techniques. J Endod 1999 May;25(5):332-335
Antimicrobial activity of 2.5% sodium hypochlorite and 0.2% chlorhexidine gluconate separately and combined, as endodontic irrigants. J Endod 1998 Jul;24(7):472- 476
Comparative investigation of the toxic and necrotic tissue dissolving effects of different endodontic irrigants. J Dent Faculty Ìstanbul Uni 1998;32:87-94
Elimination of infection in pulpectomized deciduous teeth: short-term study using iodoform paste. J Endod 1994 May;20(5):233-235
Evaluation of the effectiveness of sodium hypochlorite used with three irrigation methods in the elimination of Enterococcus faecalis from the root canal, in vitro. Int Endod J 1997 Jul;30(4):279-282
The antimicrobial effect of calcium hydroxide in root canals pretreated with 5% iodine potassium iodide. Endod Dent Traumatol 1999 Oct;15(5):205- 209
Antimicrobial evaluation of calcium hydroxide in infected dentinal tubules. J Endod 1999 Jun;25(6):351-353
Microorganisms from canals of root-filled teeth with periapical lesions. Int Endod J 2003 Jan;36(1):1-11
Evaluation of cytotoxicity of MTAD using the MTT-Tetrazolium method. J Endod 2003 Oct;29(10):654-657
Effect of MTAD on Enterococcus faecalis-contaminated root canals of extracted human teeth. J Endod 2003 Sep;29(9):576-579
The antimicrobial effect of Biopure MTAD on eight strains of Enterococcus faecalis: an in vitro investigation. J Endod 2007 Nov;33(11):1352-1354
Killing of Enterococcus faecalis by MTAD and chlorhexidine digluconate with or without cetrimide in the presence or absence of dentine powder or BSA. J Endod 2006 Feb;32(2):138-141
An evaluation of microbial leakage after using MTAD as a final irrigation. J Endod 2007 Feb;33(2):173-176
An in vitro comparision of the antimicrobial effects of various endodontic medicaments on Enterococcus faecalis. J Endod 2007 May;33(5):567-569
Comparative evaluation of endodontic irrigants against Enterococcus faecalis Biofilms. J Endod 2006 Jun;32(6):527-531
Comparision of the antimicrobial efficacy of 1.3% NaOCl/BioPure MTAD to 5.25% NaOCl/15% EDTA for root canal irrigation. J Endod 2007 Jan;33(1):48-51
The antimicrobial effect of MTAD, sodium hypochlorite, doxycycline, and citric acid on Enterococcus faecalis. J Endod 2007 Jan;33 (1):28-30
Brazilian propolis: physicochemical properties, plant origin and antibacterial activity on periodontopathogens. Phytother Res 2003 Mar;17(3):285-289
A comparative study of Propolis and saline application on human dentin: A SEM study. Indian J Dent Res 2001 Jan-Mar;12(1):21-27
Comparison of sodium hypochlorite, propolis and saline as root canal irrigants: a pilot study. Saudi Dent J 2003 May-Aug;15(2):100-103
Ambrosano GM, Park YK, Cury JA. In vitro antimicrobial activity of Propolis and Arnica Montana against oral pathogens. Arch Oral Biol 2000 Feb;45(2):141- 148
Efficacy of propolis as an intracanal medicament against Entercoccus faecalis. Gen Dent 2006 Sep-Oct;54(5):319-322
The treatment of simple pulp gangrene with the epitherapeutic agent “Propolis” [Tratamentul gangrenei pulpare simple cu produsul apiterapic “Propolis”]. Rev Chir Oncol Radiol O R L Oftalmol Stomatol Ser Stomatol 1986 Apr-Jun;33(2):115-117. (Rom)
Preliminary report on the effects of propolis on wound healing in the dental pulp. Z Naturforsch C 1998 Nov- Dec;53(11-12):1045-1048
Effect of propolis on human fibroblasts from the pulp and periodontal ligament. J Endod 2004 May;30(5):359-361.