Evaluation and Comparison of Biological Cleaning Efficacy of Two Endofiles and Irrigants as Judged by Microbial Quantification in Primary Teeth – An In Vivo Study
Varun Goyal, Asha Singh, Chetan Bhat
Keywords :
Post and core, apexogenesis, reinforced tooth
Citation Information :
Goyal V, Singh A, Bhat C. Evaluation and Comparison of Biological Cleaning Efficacy of Two Endofiles and Irrigants as Judged by Microbial Quantification in Primary Teeth – An In Vivo Study. Int J Clin Pediatr Dent 2009; 2 (3):15-22.
The endodontic triad comprises of cleaning and shaping, disinfection and obturation. Success of root canal therapy is majorly achieved by proper cleaning and shaping. However, elimination of bacterial contaminants as well as necrotic debris of the canals requires the adjunctive use of irrigants.
To achieve a satisfactory biological and mechanical preparation proper selection of endodontic instruments and irrigants is necessary.
In this study we are comparing and evaluating cleaning efficacy of endofiles (K-files and handprotapers) and root canal irrigants (sodium hypochlorite and chlorhexidine) by microbial quantification. Root canal samples were collected in autoclavable bottles containing transport media (nutrient broth) and samples were cultured in tryptose soya agar at incubation temperature of 37°C for 24-48 hours and colonies were counted with digital colony counter.
The significance of this study is to help the clinician select proper instrument and irrigant which minimize the failure rate of root canal treatment for the benefit of patients.
Walker A. Definite and dependable therapy for pulpless teeth. J Am Dent Assoc 1936;23:14-18.
Walton RE. Histologic evaluation of different methods of enlarging the pulp canal space. J Endod 1976 Oct;2(10): 304-311.
Allison Da. Weber CR, Walton RE. The influence of the method of canal preparation on the quality of apical and coronal obturation. J Endod 1979 Oct;5(10):298-304.
Martin MP. “Irrigation investigation in endodontics, master's theses, Paris, France Paris VII 1980.
Parsons GJ, Patterson SS, Miller CH, Katz S, Kafrawy AH, Newton CW. Uptake and release of chlorhexidine by bovine pulp and dentin specimens and their subsequent acquisition of antibacterial properties. Oral Surg Oral Med Oral Pathol 1980 May;49(5):455-459.
Forrester DJ, Wagner ML, James F. Pediatric dental medicine. Philadelphia: Lea and Fabringer Co.; 1981. p. 324-340.
Walia HM, Brantley WA, Gerstein H. An initial investigation of the bending and torsional properties of Nitinol root canal files. J Endod 1988 Jul;14(7):346-351.
McGreevey E. Investigation of profile series 29..04 taper rotary instruments. Boston Boston University:, Master Thesis; 1995.
Yesilsoy C, Whitaker E, Cleveland D, Philips E, Trope M. Antimicrobial and toxic effects of established and potential root canal irrigants. J Endod 1995 Oct;21(10):513-515.
Lekshmy DS, Kamath PM. Antimicrobial efficacy of 0.2 and 2% chlorhexidine and sodium hypochlorite as root canal irrigant: An in vivo study. Endodontology 2001;13:57-62.
Hoskinson SE, Ng YL, Hoskinson AE, Moles DR, Gulabivala K. A retrospective comparison of outcome of root canal treatment using two different protocols. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2002 Jun;93(6):705-715.
Kim S. Modern endodontic practice: instruments and techniques. Dent Clin North Am 2004 Jan;48(1):1-9.
Hülsmann M, Peters OA, and Paul MH. Dummer Mechanical preparation of root canals: shaping goals, techniques and means. Endodontic Topics 2005;10:30-76.
Kuo CI, Wang YL, Chang HH, Huang GF, Lin CP, Guo MK. Application of Ni-Ti rotary files for pulpectomy in primary molars. J Dent Sci 2006;1:10-15.