International Journal of Clinical Pediatric Dentistry

Register      Login

VOLUME 17 , ISSUE 2 ( February, 2024 ) > List of Articles

ORIGINAL RESEARCH

Comparative Evaluation of Amniotic Membrane Derivative, Chitosan with Mineral Trioxide Aggregate, Diode Laser, and Ferric Sulfate as Pulpotomy Agents in Human Primary Molars: An In Vivo Study

Vibha Chadak Lahoti, Pratik Lahoti, Lakshmi Madhuri Gundreddy, Ravinder Puppala, Valasingam Sandeep, Balaji Kethineni

Keywords : Amniotic membrane derivative, Chitosan with mineral trioxide aggregate, Diode laser, Ferric sulfate, Pulpotomy

Citation Information : Lahoti VC, Lahoti P, Gundreddy LM, Puppala R, Sandeep V, Kethineni B. Comparative Evaluation of Amniotic Membrane Derivative, Chitosan with Mineral Trioxide Aggregate, Diode Laser, and Ferric Sulfate as Pulpotomy Agents in Human Primary Molars: An In Vivo Study. Int J Clin Pediatr Dent 2024; 17 (2):153-157.

DOI: 10.5005/jp-journals-10005-2767

License: CC BY-NC 4.0

Published Online: 22-04-2024

Copyright Statement:  Copyright © 2024; The Author(s).


Abstract

Aim: The aim of the present study was to compare the clinical and radiographic success of amniotic membrane derivative (AMD), chitosan with mineral trioxide aggregate (C-MTA), diode laser (DL), and ferric sulfate (FS) as pulpotomy agents in human primary molars. Materials and methods: In this present study, pulpotomies were performed on 48 primary molars in 30 children aged between 4 and 8 years (12 teeth in each group). Following the pulpotomy procedure, teeth were evaluated clinically and radiographically at 1st, 3rd, 6th, and 9 monthly intervals. Results: After 9 months of follow-up, the clinical success was 91.6% for AMD and C-MTA and 83.3% for DL and FS. Radiographic success was 91.6, 91.6, 75, and 83.3% for AMD, C-MTA, DL, and FS groups, respectively. There is no statistically significant difference between the four groups (p > 0.05). Interpretation and conclusion: Results of our study showed that both AMD and C-MTA were equally successful compared to traditional agents like laser and ferric sulfate as pulpotomy agents. Clinical significance: Amniotic membrane derivative (AMD) and C–MTA are alternative biomimetic pulpotomy agents that can be used in pediatric primary tooth pulpotomies.


PDF Share
  1. Fuks AB. Vital pulp therapy with new materials for primary teeth: new directions and treatment perspectives. J Endod 2008;34(7 Suppl):S18–S24. DOI: 10.1016/j.joen.2008.02.031
  2. Fuks AB. Current concepts in vital primary pulp therapy. Eur J Paediatr Dent 2002;3(3):115–120.
  3. Ranly DM. Pulpotomy therapy in primary teeth: new modalities for old rationales. Pediatr Dent 1994:16(6):403–409.
  4. Guideline on Pulp Therapy for Primary and Immature Permanent Teeth. AAPD reference manual. 36(6):14–15.
  5. Milnes AR. Is formocresol obsolete? A fresh look at the evidence concerning safety issues. J Endod 2008;34(7 Suppl):S40–S46. DOI: 10.1016/j.joen.2008.03.008
  6. Chandrashekhar S, Shashidhar J. Formocresol, still a controversial material for pulpotomy: a critical literature review. J Restor Dent 2014;2(3):114–124. DOI: 10.4103/2321-4619.143594
  7. Stuart JR, Stefan SW, Dennis PT, et al. Effect of PRP on bone growth and osseointegration in human maxillary sinus grafts: three bilateral case reports. Int J Periodont Rest Dent 2002:22(1):45–53.
  8. Faulk WP, Matthews R, Stevens PJ, et al. Human amnion as an adjunct in wound healing. Lancet 1980;1(8179):1156–1158. DOI: 10.1016/s0140-6736(80)91617-7
  9. Sikkerimath BC, Dandagi S, Gudi SS, et al. Comparison of vestibular sulcus depth in vestibuloplasty using standard Clark's technique with and without amnion as graft material. Ann Maxillofac Surg 2012;2(1):30–35. DOI: 10.4103/2231-0746.95313
  10. Talmi YP, Sigler L, Inge E, et al. Antibacterial properties of human amniotic membranes. Placenta 1991;12(3):285–288. DOI: 10.1016/0143-4004(91)90010-d
  11. Toda A, Okabe M, Yoshida T, et al. The potential of amniotic membrane/amnion-derived cells for regeneration of various tissues. J Pharmacol Sci 2007;105(3):215–228. DOI: 10.1254/jphs.cr0070034
  12. Koizumi N, Inatomi T, Sotozono C, et al. Growth factor mRNA and protein in preserved human amniotic membrane. Curr Eye Res 2000;20(3):173–177.
  13. Samandari MH, Adibi S, Khoshzaban A, et al. Human amniotic membrane, best healing accelerator, and the choice of bone induction for vestibuloplasty technique (an animal study). Transplant Res Risk Manage 2011;3:1–8. DOI: 10.2147/TRRM.S11741
  14. Gurinsky B. A novel dehydrated amnion allograft for use in the treatment of gingival recession: an observational case series. J Implant Advanced Clin Dent 2009;1(1).
  15. Spin-Neto R, de Freitas RM, Pavone C, et al. Histological evaluation of chitosan-based biomaterials used for the correction of critical size defects in rat's calvaria. J Biomed Mater Res A 2010;93(1):107–114. DOI: 10.1002/jbm.a.32491
  16. Budiraharjo R, Neoh KG, Kang ET, et al. Bioactivity of novel carboxymethyl chitosan scaffold incorporating MTA in a tooth model. Int Endod J 2010;43(10):930–939. DOI: 10.1111/j.1365-2591.2010.01771.x
  17. Odabaş ME, Cinar C, Tulunoğlu O, et al. New haemostatic agent's effect on the success of calcium hydroxide pulpotomy in primary molars. Pediatr Dent 2011:33(7):529–534.
  18. Chevrier A, Hoemann CD, Sun J, et al. Chitosan-glycerol phosphate/blood implants increase cell recruitment, transient vascularization and subchondral bone remodeling in drilled cartilage defects. Osteoarthritis Cartilage 2007;15(3):316–327. DOI: 10.1016/j.joca.2006.08.007
  19. Klokkevold PR, Newman MG. Current status of dental implants: a periodontal perspective. Int J Oral Maxillofac Implant 2000;15(1):56–65.
  20. Kim IY, Seo SJ, Moon HS, et al. Chitosan and its derivatives for tissue engineering applications. Biotechnol Adv 2008;26(1):1–21. DOI: 10.1016/j.biotechadv.2007.07.009
  21. Agamy HA, Bakry NS, Mounir MF, et al. Comparison of mineral trioxide aggregate and formocresol as pulp-capping agents in pulpotomized primary teeth. Pediatr Dent 2004;26(4):302–309.
  22. Naik S, Hegde AH. Mineral trioxide aggregate as a pulpotomy agent in primary molars: an in vivo study. J Indian Soc Pedod Prev Dent 2005;23(1):13–16. DOI: 10.4103/0970-4388.16020
  23. Farsi N, Alamoudi N, Balto K, et al. Success of mineral trioxide aggregate in pulpotomized primary molars. J Clin Pediatr Dent 2005;29(4):307–311. DOI: 10.17796/jcpd.29.4.n80t77w625118k73
  24. Maroto M, Barberia E, Vera V, et al. Mineral trioxide aggregate as pulp dressing agent in pulpotomy treatment of primary molars: 42-month clinical study. Am J Dent 2007;20(5):283–286.
  25. Holan G, Eidelman E, Fuks AB. Long-term evaluation of pulpotomy in primary molars using mineral trioxide aggregate or formocresol. Pediatr Dent 2005:27(2):129–136.
  26. Miller M, Truhe T. Lasers in dentistry: an overview. J Am Dent Assoc 1993;124(2):32–35. DOI: 10.14219/jada.archive.1993.0034
  27. Shoji S, Nakamura M, Horiuchi H. Histopathological changes in dental pulps irradiated by CO2 laser: a preliminary report on laser pulpotomy. J Endod 1985;11(9):379–384. DOI: 10.1016/S0099-2399(85)80024-8
  28. Toomarian L, Fekrazad R, Sharifi D, et al. Histopathological evaluation of pulpotomy with Er,Cr:YSGG laser vs formocresol. Lasers Med Sci 2008;23(4):443–450. DOI: 10.1007/s10103-007-0505-3
  29. Miserendino LJ, Neiburger EJ, Walia H, et al. Thermal effects of continuous wave CO2 laser exposure on human teeth: an in vitro study. J Endod 1989;15(7):302–305. DOI: 10.1016/S0099-2399(89)80051-2
  30. McNally KM, Gillings BR, Dawes JM. Dye-assisted diode laser ablation of carious enamel and dentine. Aust Dent J 1999;44(3):169–175. DOI: 10.1111/j.1834-7819.1999.tb00218.x
  31. Utsunomiya T. A histopathological study of the effects of low-power laser irradiation on wound healing of exposed dental pulp tissues in dogs, with special reference to lectins and collagens. J Endod 1998;24(3):187–193. DOI: 10.1016/S0099-2399(98)80181-7
  32. Saltzman B, Sigal M, Clokie C, et al. Assessment of a novel alternative to conventional formocresol-zinc oxide eugenol pulpotomy for the treatment of pulpally involved human primary teeth: diode laser-mineral trioxide aggregate pulpotomy. Int J Paediatr Dent 2005;15(6):437–447. DOI: 10.1111/j.1365-263X.2005.00670.x
  33. Golpayegani MV, Ansari G, Tadayon N. Clinical and radiographic success of low level laser therapy (LLLT) on primary molars pulpotomy. Res J Biol Sci 2010;42(5):51–55.
  34. Schtodet U. Effect of an extra-pulpal blood clot on healing following experimental pulpotomy and capping with calcium hydroxide. Odontol Revy 1973;24(3):257–268.
  35. Fei AL, Udin RD, Johnson R. A clinical study of ferric sulfate as a pulpotomy agent in primary teeth. Pediatr Dent 1991;13(6):327–332.
  36. Fuks AB, Eidelman E, Cleaton-Jones P, et al. Pulp response to ferric sulfate, diluted formocresol and IRM in pulpotomized primary baboon teeth. J Dent Child 1997;64(4):254–258.
  37. Smith NL, Seale NS, Nunn ME. Ferric sulfate pulpotomy in primary molars: a retrospective study. Pediatr Dent 2000:22(3):192–199.
  38. Neamatollahi H, Tajik A. Comparison of clinical and radiographic success rates of pulpotomy in primary molars using formocresol, ferric sulfate and mineral trioxide aggregate. J Dent 2006;3(1):6–14.
PDF Share
PDF Share

© Jaypee Brothers Medical Publishers (P) LTD.