Objectives: To study the influence of nanoparticles of hydroxyapatite, zirconia, and glass on the wear and the microhardness of the organic matrix of experimental dental composite resin. Materials and methods: The dental composite resin matrix was fabricated from bisphenol A-glycidyl methacrylate (Bis-GMA) (40 wt%), triethylene glycol dimethacrylate (TEGDMA) (36 wt%), and camphorquinone (0.4 wt%). Nanohydroxyapatite, glass, and zirconia fillers were silane treated. Nano-hydroxyapatite, glass, and zirconia were incorporated at three different concentrations. The polymerization of the dental composite resin was done using a light curing unit. Experimental dental composite resins were evaluated for wear and microhardness. The data were analyzed by one-way analysis of variance (ANOVA) test. Results: The experimental dental composite resin composed of 32% of nanohydroxyapatite, 27% of zirconia, and 19% of glass as filler showed the minimum amount of wear. The Vickers hardness (VHN) number was observed to be minimum for the experimental dental composite resin composed of 24.1% of nanohydroxyapatite, 22% of zirconia, and 14.5% of glass. Conclusion: The inclusion of 32% nanohydroxyapatite, 27% of zirconia, and 19% of glass as filler into the experimental dental composite resin decreased the wear and increased the hardness.