International Journal of Clinical Pediatric Dentistry

Register      Login

VOLUME 16 , ISSUE 6 ( November-December, 2023 ) > List of Articles

ORIGINAL RESEARCH

Development of an Advanced Material for Obturation in Primary Teeth Based on the Microbiological Analysis of the Antimicrobial Efficacy of Various Concentrations: An In Vitro Study

Lavanya Govindaraju

Keywords : Child dentistry, Novel, Obturating material, Pediatric endodontics, Pulpectomy

Citation Information : Govindaraju L. Development of an Advanced Material for Obturation in Primary Teeth Based on the Microbiological Analysis of the Antimicrobial Efficacy of Various Concentrations: An In Vitro Study. Int J Clin Pediatr Dent 2023; 16 (6):800-803.

DOI: 10.5005/jp-journals-10005-2721

License: CC BY-NC 4.0

Published Online: 01-02-2024

Copyright Statement:  Copyright © 2023; The Author(s).


Abstract

Introduction: Endodontics in primary teeth has brought a revolution in preserving the primary teeth until it is physiological exfoliation. However, reinfection and failure of the pulpectomy-treated teeth should not be neglected. This raises the need for the best root canal filling material with the strongest antibacterial capabilities for primary teeth. The current study set out to assess the antimicrobial efficacy of different concentrations of the novel obturating material against Streptococcus mutans and Enterococcus faecalis, the two most common microorganisms found in primary tooth infections. Materials and methods: The antimicrobial efficacy of the novel obturating material was evaluated against Streptococcus mutans and Enterococcus faecalis using Mueller–Hinton agar (MHA) for 24 hours at 37°C. Twelve distinct concentrations of the material were prepared by varying the proportions of calcium hydroxide, zinc oxide, and metronidazole. After a day, the zone of inhibition was measured in millimeters. After entering the input into a Microsoft Excel spreadsheet, the values underwent statistical analysis. Results: The mean zone of inhibition against Streptococcus mutans and Enterococcus faecalis was greater with 2% 60–40 concentration of the novel obturating material. However, no statistically significant difference was noted (p = 0.74, 0.22, respectively). Conclusion: The most effective formulation of the novel obturating material with the greatest antimicrobial property was determined to be 2% 60–40 concentration of metronidazole, calcium hydroxide, and zinc oxide.


PDF Share
  1. Tronstad L. Recent development in endodontic research. Eur J Oral Sci 1992;100(1):52–59. DOI: 10.1111/j.1600-0722.1992.tb01809.x
  2. Goerig AC, Camp JH. Root canal treatment in primary teeth: a review. Pediatr Dent 1983;5(1):33–37.
  3. Garcia-Godoy F. Evaluation of an iodoform paste in root canal therapy for infected primary teeth. ASDC J Dent Child 1987;54(1):30–34.
  4. Lemos SS, Cesar DE, Procópio SW, et al. Qualitative and quantitative molecular analysis of bacteria in root canals of primary teeth with pulp necrosis. Braz Oral Res 2020;34:e093. DOI: 10.1590/1807-3107bor-2020.vol34.0093
  5. Rifkin A. The root canal treatment of abscessed primary teeth–a three to four year follow-up. ASDC J Dent Child 1982;49(6):428–431.
  6. Reddy S, Ramakrishna Y. Evaluation of antimicrobial efficacy of various root canal filling materials used in primary teeth: a microbiological study. J Clin Pediatr Dent 2007;31(3):193–198. DOI: 10.17796/jcpd.31.3.t73r4061424j2578
  7. Kriplani R, Thosar N, Baliga MS, et al. Comparative evaluation of antimicrobial efficacy of various root canal filling materials along with aloevera used in primary teeth: a microbiological study. J Clin Pediatr Dent 2013;37(3):257–262. DOI: 10.17796/jcpd.37.3.j62u53q2300484x5
  8. Tchaou WS, Turng BF, Minah GE, et al. Inhibition of pure cultures of oral bacteria by root canal filling materials. Pediatr Dent 1996;18(7):444–449.
  9. Pabla T, Gulati MS, Mohan U. Evaluation of antimicrobial efficacy of various root canal filling materials for primary teeth. J Indian Soc Pedod Prev Dent 1997;15(4):134–140.
  10. Ninomiya J, Sugawara M, Yamachika K, et al. Studies on antimicrobial activity of Vitapex in vitro. J Conserv Dent 1980;23:625–630.
  11. Nurko C, Ranly DM, García-Godoy F, et al. Resorption of a calcium hydroxide/iodoform paste (Vitapex) in root canal therapy for primary teeth: a case report. Pediatr Dent 2000;22(6):517–520.
  12. Spedding RH. Incomplete resorption of resorbable zinc oxide root canal fillings in primary teeth: report of two cases. ASDC J Dent Child 1985;52(3):214–216.
  13. Hendry JA, Jeansonne BG, Dummett CO Jr, et al. Comparison of calcium hydroxide and zinc oxide and eugenol pulpectomies in primary teeth of dogs. Oral Surg Oral Med Oral Pathol 1982;54(4):445–451. DOI: 10.1016/0030-4220(82)90394-2
  14. Cox ST Jr, Hembree JH Jr, McKnight JP. The bactericidal potential of various endodontic materials for primary teeth. Oral Surg Oral Med Oral Pathol 1978;45(6):947–954. DOI: 10.1016/s0030-4220(78)80017-6
  15. Kayaoglu G, Ørstavik D. Virulence factors of Enterococcus faecalis: relationship to endodontic disease. Crit Rev Oral Biol Med 2004;15(5):308–320. DOI: 10.1177/154411130401500506
  16. Coll JA, Sadrian R. Predicting pulpectomy success and its relationship to exfoliation and succedaneous dentition. Pediatr Dent 1996;18(1):57–63.
  17. Schleifer KH, Kilpper-Bälz R. Transfer of Streptococcus faecalis and Streptococcus faecium to the genus Enterococcus nom. rev. as Enterococcus faecalis comb. nov. and Enterococcus faecium comb. nov. Int J Syst Evol Microbiol 1984;34(1):31–34.
  18. Ryan KJ, Ray CG. Medical Microbiology. McGraw Hill; 2004.
PDF Share
PDF Share

© Jaypee Brothers Medical Publishers (P) LTD.