Aim: The purpose of this study was to compare the reduction in salivary mutans streptococci counts and to evaluate any change in the salivary pH values after the use of oral probiotics, chlorhexidine, and herbal mouthrinse.
Method: Oral probiotics, chlorhexidine, and herbal mouthrinse [Hiora®] were evaluated for their efficacy against S. mutans in 75 caries-active children, aged 6–14 years.
Results: The values obtained were subjected to statistical analysis. Oral probiotics showed lesser reduction in the S. mutans count as compared to chlorhexidine but the values were statistically insignificant. Herbal mouthrinse was not as effective as the oral probiotics or chlorhexidine in reducing the S. mutans count.
Conclusion: Oral probiotics show a similar efficacy as the gold standard, chlorhexidine, in reduction of oral S. mutans.
Fardal O, Turnbull RS. A review of the literature on use of chlorhexidine in dentistry. JADA 1986;112:863–869. DOI: 10.14219/jada.archive.1986.0118
Nagappan N, John J. Antimicrobial efficacy of herbal and chlorhexidine mouthrinse - a systematic review. IOSR-JDMS 2012;2(4):05–10.
Mishra R, Tandon S, Rathore M, et al. Antimicrobial efficacy of probiotic oral rinses against Candida albicans in children: a randomized clinical trial. Int J Clin Pediatr Dent 2016;9(1):25–30. DOI: 10.5005/jp-journals-10005-1328
Renuka S, Muralidharan NP. Comparison in benefits of herbal mouthwashes with chlorhexidine mouthwash: a review. Asian J Pharm Clin Res 2017;10(2):3–7. DOI: 10.22159/ajpcr.2017.v10i2.13304
Hotel AC, Cordoba A. Health and nutritional properties of probiotics in food including powder milk with live lactic acid bacteria. Prevention 2001;5(1):1–34.
Cortes- Dorantes N, Ruiz-Rodríguez MS, Karakowsky-Kleiman L, et al. Probiotics and their effect on oral bacteria count in children: a pilot study. Eur J Pediatr Dent 2015;16(1):56–60.
Zahradnik RT, Magnusson I, Walker C, et al. Preliminary assessment of safety and effectiveness in human of ProBiora3, a probiotic mouthwash. J Appl Microbiol 2009;682–690. DOI: 10.1111/j.1365-2672.2009.04243.x
Sundaram M, Nayak UA, Ramalingam K, et al. A comparative evaluation of Oratest with the microbiological method of assessing caries activity in children. J Pharm Bioallied Sci 2013;5(Suppl. S1):5–9. DOI: 10.4103/0975-7406.113283
Alaluusua S, Renkonen O. Streptococcus mutans establishment and dental caries experience in children from 2 to 4 years old. Scand J Dent Res 1983;91(6):453–457. DOI: 10.1111/j.1600-0722.1983.tb00845.x
Rogers AH. The proportional distribution and characteristics of streptococci in human dental plaque. Caries Res1969;3:238–248. DOI: 10.1159/000259598
Pannu P, Gambhir R, Sujlana A. Correlation between the salivary Streptococcus mutans levels and dental caries experience in adult population of Chandigarh, India. Eur J Dent 2013;7(2):191–195. DOI: 10.4103/1305-7456.110169
Kristofferson K, Axelsson P, Birkhed D, et al. Caries prevalence, salivary streptococcus mutans and dietary scores in 13 years old Swedish school children. Community Dent Oral Epidemiol 1986;14(4):202–205. DOI: 10.1111/j.1600-0528.1986.tb01535.x
Maltz M, Zicfcert I, Krasse B. Effect of intensive treatment with chlorhexidine and number of Streptococcus mutans in saliva. Scand J Dent Res 1981:89:445-449. DOI: 10.1111/j.1600-0722.1981.tb01707.x
Järvinen H, Tenovuo J, Huovinen P. In vitro susceptibility of Streptococcus mutans to chlorhexidine and six other antimicrobial agents. Antimicrob Agents Chemother 1993;37(5): 1158–1159. DOI: 10.1128/aac.37.5.1158
Subramaniam P, Gupta T. Efficacy of a herbal mouthrinse on oral microbial load in down syndrome children. Int J Herb Med 2013;1(4):50–54.
Sharma A, Agarwal N, Anand A, et al. To compare the effectiveness of different mouthrinses on Streptococcus mutans count in caries active children. J Oral Biol Craniofac Res 2018;8(2):113–117. DOI: 10.1016/j.jobcr.2018.05.002
Ramamurthy J. Comparison of effect of Hiora mouthwash and chlorhexidine mouthwash in gingivitis patients: a clinical trial. Asian J Pharm Clin Res 2018;11(7):84. DOI: 10.22159/ajpcr.2018.v11i7.24783
Jaidka S, Somani R, Bajaj N, et al. Comparative evaluation of various mouthwashes for their effect on oral health: an in- vivo study. Int J Oral Care.
Gupta R, Yadav OP, Khan M, et al. Comparative evaluation of efficacy of Hiora, Terminalia chebula and Chlorhexidine as mouthwash on dental plaque. J Dent Health Oral Disord Ther 2017;8(4):00290. DOI: 10.15406/jdhodt.2017.08.00290
Laleman I, Yilmaz E, Ozcelik O, et al. The effect of a streptococci containing probiotic in periodontal therapy: a randomized controlled trial. J Clin Periodontol 2015; 42:1032–1041. DOI: 10.1111/jcpe.12464
Twetman S, Stecksen- Blicks C. Probiotics and oral health effects in children. Int J Paed Dent 2008;18:3–10. DOI: 10.1111/j.1365-263X.2007.00885.x
Jain P, Sharma P. Probiotics and their efficacy in improving oral health: a review. J App Pharm Sci 2012;2(11):151–163. DOI: 10.7324/JAPS.2012.21128
Hedayati-Hajikand T, Lundberg U, Eldh C, et al. Effect of probiotic chewing tablets on early childhood caries- a randomized controlled trial. BMC Oral Health 2015;15:112. DOI: 10.1186/s12903-015-0096-5
Elahi S, Pang G, Ashman R, et al. Enhanced clearance of Candida albicans from the oral cavities of mice following oral administration of Lactobacillus acidophilus. Clin Exp Immunol 2005;141(1):29–36. DOI: 10.1111/j.1365-2249.2005.02811.x
Stamatova I, Kari K, Meurman JH. In vitro evaluation of antimicrobial activity of putative probiotic lactobacilli against oral pathogens. Int J Probiotics Prebiotics 2007;2:225–232.