International Journal of Clinical Pediatric Dentistry

Register      Login

SEARCH WITHIN CONTENT

FIND ARTICLE

Volume / Issue

Online First

Archive
Volume  17 (2024)
Volume  16 (2023)
Volume  15 (2022)
Volume  14 (2021)
Volume  13 (2020)
Volume  12 (2019)
Volume  11 (2018)
Volume  10 (2017)
Volume  9 (2016)
Volume  8 (2015)
Volume  7 (2014)
Volume  6 (2013)
Volume  5 (2012)
Volume  4 (2011)
Volume  3 (2010)
Volume  2 (2009)
Volume  1 (2008)
Related articles

VOLUME 15 , ISSUE S2 ( Special Issue-2 (Cariology), 2022 ) > List of Articles

RESEARCH ARTICLE

A Comparative Evaluation of Microhardness and Chemical Structure of Radicular Dentin with Two Combinations of TAP and MTAP: An In Vitro Study

Muthumula Daneswari, Nagireddy Venugopal Reddy, Annie P Chris, Nikhila V Reddy, Saigeeta Kondamadugu, P Niharika

Citation Information :

DOI: 10.5005/jp-journals-10005-2170

License: CC BY-NC 4.0

Published Online: 16-03-2022

Copyright Statement:  Copyright © 2022; The Author(s).


Abstract

Background: The success of regenerative endodontics depends on various factors and the most vital being the complete eradication of microorganisms in the dentinal tubules. This could lead to changes that leave the radicular dentin prone to fracture. Aim: The purpose of the present study is to investigate the effects of triple antibiotic paste (TAP) and modified triple antibiotic paste (MTAP) of different concentrations on the microhardness and chemical structure of radicular dentin. Materials and methods: Human root cylinders were instrumented and randomized into four treatment groups and an untreated control group. Two treatment groups received 1 g/mL TAP or MTAP, and the other two treatment groups received 1 mg/mL methylcellulose-based TAP or MTAP. Cylinders were stored at 100% relative humidity for 4 weeks. Each root cylinder was subjected to a microhardness test before and after treatment. Different sets of radicular dentin specimens were treated as mentioned previously, and were examined using attenuated total reflection Fourier transform infrared spectroscopy. Results: Significant reductions in microhardness of treated groups was noticed when compared to untreated control roots at 1,000 and/or 500 µm from the pulp-dentin interface. Conclusion: The use of 1 mg/mL methylcellulose-based TAP and MTAP may minimize the reduction in microhardness of roots compared with the currently used 1 g/mL concentration of these antibiotics.

HTML PDF Share
  1. Sangappa SK, Javanaiahar N, Kumar AP, et al. Regenerative endodontic: current progress. IOSR J Dent Med Sci (IOSR-JDMS) 2014;13(4):88–95. e-ISSN: 2279-0853, p-ISSN: 2279-0861. www.iosrjournals.org
  2. Nosrat A, Seifi A, Asgary S. Regenerative endodontic treatment for necrotic immature permanent molars: a review and report of two cases with a new material. J Endod 2011;37(4):562–567. DOI:10.1016/j.joen.2011.01.011
  3. Baumgartner JC, Siqueira JF, Jr, Sedgley CM, et al.Microbiology of endodontic disease. In: Ingle JI, Bakland LK, Baumgartner JC, editors. Ingle's Endodontics. Sixth ed. Hamilton, Canada: BC Decker; 2008. pp. 221–308.
  4. Verma R, Sharma DS, Pathak AK. Antibacterial efficacy of pastes against E faecalis in primary root dentin: A confocal microscope study. Journal of Clinical Pediatric Dentistry. 2015;39(3):247–254. DOI: https://doi.org/10.17796/1053-4628-39.3.247
  5. Ashish PM, Pravin PG, Heeresh SK, et al. Role of triple antibiotic paste in revascularization and endodontics – A review. Int J Contemp Med Res 2015;2(2):21–25.
  6. Lee BN, Moon JW, Chang HS, et al. A study of the regenerative endodontic treatment procedure. Restor Dent Endod 2015;40(4):322–327. DOI: https://doi.org/10.5395/rde.2015.40.3.179
  7. Haapasalo M, Udnæs T, Endal U. Persistent, recurrent and acquired infection of the root canal system posttreatment. Endod Top 2003;6(1):29–56. DOI:10.1111/j.1601-1546.2003.00041.x
  8. Yelick PC, Vacanti JP. Bioengineered teeth from tooth bud cells. Dent Clin North Am 2006;50:191–203. DOI: 10.1016/j.cden.2005.11.005
  9. Dummer PM, Hicks R, Huws D. Clinical signs and symptoms of disease. Int Endod J1980;13(1):27–35. DOI: 10.1111/j.1365-2591.1980.tb00834.x
  10. Trope M. Treatment of the immature tooth with a non–vital pulp and apical periodontitis. Dent Clin N Am 2010;54(2):313–324. DOI: 10.1016/j.cden.2009.12.006
  11. Peters OA, Laib A, Gohring TN, et al. Changes in root canal geometry after preparation assessed by high resolution computed tomographyJ Endod2001;27(1):1–6. DOI: 10.1097/00004770-200101000-00001
  12. Haapasalo M, Qian W, Portenier I, et al. Effects of dentin on the antimicrobial properties of endodontic medicaments. J Endod2007;33(8):917–925. DOI: 10.1016/j.joen.2007.04.008
  13. Sedgley CM, Lee EH, Martin MJ, et al. Antibiotic resistance gene transfer between Streptococcus gordonii and Enterococcus faecalis in root canals of teeth-ex vivo. J Endod2008;34(5):570–574. DOI: 10.1016/j.joen.2008.02.014
  14. Turk BT, Sen BH, Ozturk T. In vitro antimicrobial activity of calcium hydroxide mixed with different vehicles against Enterococcus faecalis and Candida albicans. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2009;108(2):297–301. DOI: 10.1016/j.tripleo.2009.03.029
  15. Goodson J. Pharmacokinetic principles controlling efficacy of oral therapy. J Dent Res1989;68:1625–1632. DOI: https://doi.org/10.1016/0016-2361(89)90312-8
  16. Sathorn C, Parashos P, Messer HH. How useful is root canal culturing in predicting treatment outcome?J Endod2007;33(3):220–225. DOI: 10.1016/j.joen.2006.11.006
  17. Hoshino E, Sato M, Sasano T, et al. Characterization of bacterial deposits formed in vivo on hydrogen-ion sensitive field transistor electrodes and enamel surfaces. Shika Kiso Igakkai Zasshi 1989;31(1):102–106. DOI: 10.2330/joralbiosci1965.31.102
  18. Yassen GH, Vail MM, Chu TG, et al. The effect of medicaments used in endodontic regeneration on root fracture and microhardness of radicular dentinInt Endod J 2013;46:688–695. DOI:10.1111/iej.12046
  19. Liu JY, Chen X, Yue L, et al. CXC Chemokine receptor 4 is expressed paravascularly in apical papilla and coordinates with stromal cell derived factor-1αduring transmigration of stem cells from apical papilla. J Endod 2015;41(9):1430–1436. DOI:10.1016/j.joen.2015.04.006
  20. Chaudhary S, Talwar S, Ravi R, et al. Revascularization of immature permanent tooth with periapical lesion using a new biomaterial - a case report. Int J Dent Sci Res 2013;1(1):20–22. DOI: 10.12691/ijdsr-1-1-5
  21. Yadlapati M, Souza LC, Dorn S, et al. Deleterious effect of triple antibiotic paste on human periodontal ligament fibroblasts. Int Endod J 2014;47(8):769–775. DOI: 10.1111/iej.12216
  22. Ashish M, Pravin P, Heeresh S et al. Role of triple antibiotic paste in revascularization and endodontics - a review. Int J Contemp Med Res 2015;2(2)78–81. DOI: https://doi.org/10.5395/rde.2018.43.e28
  23. Albuquerque MT, Ryan SJ, Münchow EA, et al. Antimicrobial effects of novel triple antibiotic paste-mimic scaffolds on actinomyces naeslundii biofilm. J Endod 2015;41(8):1337–1343. DOI: 10.1016/j.joen.2015.03.005
  24. Prather BT, Ehrlich Y, Spolnik K, et al. Effects of two combinations of triple antibiotic paste used in endodontic regeneration on root microhardness and chemical structure of radicular dentin. J Oral Sci 2014; 56(4):245–251. DOI: 10.2334/josnusd.56.245
  25. Nerness A, Ehrlich Y, Spolnik K, et al. Effect of triple antibiotic paste with or without ethylene diamine tetraacetic acid on surface loss and surface roughness of radicular dentin. Odontology 2015;6:2–9. DOI: 10.1007/s10266-014-0191-0
  26. Yassen GH, Eckert GH, Platt JA. Effect of intracanal medicaments used in endodontic regeneration procedures on microhardness and chemical structure of human immature radicular dentin: a n in-vitro study. J Endod 2013;39(2):269–273 DOI: 10.1016/j.joen.2012.09.020
PDF Share
PDF Share

© Jaypee Brothers Medical Publishers (P) LTD.