Root canal sealants placed in primary tooth are unique in various characteristics. Chitosan nanoparticles have gained an important milestone in research area due to its biodegradable and its bioavailability nature. Chitosan nanoparticles synthesized by ionic gelation method and studied for its physical characteristics when added with root canal sealant provides a promising result. In this study, biocompatibility nature of Chitosan nanoparticles with Vero cell line was being investigated. Several solution parameters of Chitosan nanoparticles and root canal sealants were investigated to optimize diameter of nanoparticles after being characterized to XRD, SEM, and FTIR. The Chitosan nanoparticles were found to be biocompatible to fibroblasts and on a dose-dependent manner, these can be used in combination with the root canal sealant in primary teeth.
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