Assessment of Cariogenicity by pH-value Decrement of Plaque Solution with Four Infant Milk Formulas: An In Vitro Study
Anil Pandey, Alok Avinash
Citation Information :
Pandey A, Avinash A. Assessment of Cariogenicity by pH-value Decrement of Plaque Solution with Four Infant Milk Formulas: An In Vitro Study. Int J Clin Pediatr Dent 2022; 15 (3):263-266.
Introduction: People all over the world use a wide variety of infant formulas to nourish the infants. Recent studies demonstrated the high caries-inducing potential of infant formulas. This indicates a need for awareness toward the possible role of the infant formulas in the etiology of early childhood caries (ECC). Aim: The present study was undertaken to evaluate and comparatively assess the change in plaque pH solutions after fermenting four commonly used infant milk formulas. Materials and methods: This simple randomized study was carried out on 40 healthy children aged 4–6 years old. The children received full-mouth prophylaxis before examination. They were asked not to implicate oral hygiene for 24 hours and not to have anything at least 2 hours prior the study. Supragingival plaque from the buccal surface of posterior teeth was collected using a Hu-Friedy's curette. Four commonly used infant milk formulas were prepared and given to children for rinsing. The pH of plaque samples were measured at 30 and 60 minutes at 37°C. Results: The present study showed that all of the four infant milk formulas decreased the mean pH values in plaque solutions significantly after 30 and 60 minutes of preparation. Conclusion: Our results showed that the plaque pH varied in response to the oral rinsing with the various infant formulas and most of infant formulas were able to reduce the pH significantly below the pH before the rinse. Based upon this study further evaluation of the cariogenicity of infant formulas is recommended.
Anil S, Anand PS. Early childhood caries: prevalence, risk factors, and prevention. Front Pediatr 2017;5:157. DOI: 10.3389/fped.2017.00157
Munshi AK, Kavita H, Santhi KP. Acidogenic potential of the infant milk formulas marketed in India. J Indian Soc Pedo Prev Dent 2001;19(1):1–8.
Mahejabeen R, Sudha P, Kulkarni SS, et al. Dental caries prevalence among preschool children of Hubli: Dharwad city. J Indian Soc Pedod Prev Dent 2006;24(1):19–22. DOI: 10.4103/0970-4388.22829
Tan SF, Tong HJ, Lin XY, et al. The cariogenicity of commercial infant formulas: a systematic review. Eur Arch Paediatr Dent 2016;17(3): 145–156. DOI: 10.1007/s40368-016-0228-x
Bibby BG, Volker JF, Kesteren V. Acid production and tooth decalcification by oral bacteria. J Dent Res 1941;21(1):61–72. DOI: 10.1177/00220345420210011001
Chaudhary SD, Chaudhary M, Singh A, et al. An assessment of the cariogenicity of commonly used infant milk formulae using microbiological and biochemical methods. Int J Dent 2011:320798. DOI: 10.1155/2011/320798
Edgar WM, Geddes DAM. Plaque acidity models used for cariogenicity testing. J Dent Res 1986;65:1498–1502.
Tandon S, Shankar M, Gopinath VK, et al. Comparative evaluation of cariogenic potential of different types of milk and milk formula in pre-school children. J Indian Soc Pedo Prev Dent 1997; 15(2):64–68.
Erickson PR Mazhari E. Investigation of the role of human breast milk in caries development Pediatr Dent 1999;21(2):86–90.
Tinanoff N. Introduction to the early childhood caries conference: initial description and current understanding. Community Dent Oral Epidemiol 1998;26(1 Suppl):5–7. DOI: 10.1111/j.1600-0528.1998.tb02089.x
Danchaivijitr A, Nakornchai S, Thaweeboon B, et al. The effect of different milk formulas on dental plaque pH. Int J Paediatr Dent 2006;16(3):192–198. DOI: 10.1111/j.1365-263X.2006.00722.x
Fejerskov O, Scheie AA, Manji F. The effect of sucrose on plaque pH in the primary and permanent dentition of caries-inactive and -active Kenyan children. J Dent Res 1992;71(1):25–31. DOI: 10.1177/00220345920710010401
Jensen ME. Human dental plaque pH following exposure to fermentable carbohydrate. PhD Thesis at University of Minnesota 1982.
Tinanoff N, Association of diet with dental caries in preschool children. Dent Clin North Am 2005;49(4):725–737. DOI: 10.1016/j.cden.2005.05.011
Masih U, Prabhakar M, Joshi JL, et al. A comparative study of acidogenic potential of milk and commonly used milk formula. Int J Dent Clin 2010;2(4):30–32.
Anderson P, Hector MP, Rampersad MA. Critical pH in resting and stimulated whole saliva in groups of children and adults. Int J Paediatr Dent 2001;11(4):266–273. DOI: 10.1046/j.1365-263x.2001.00293.x
Saigal A, Tewari A. Cariogenicity of milk, apple juice and shikanjvi, a dental plaque study. J Am Dent Assoc 1979;80(51–52):373–377.
Schatele CF, Jensen ME. Comparison of methods for monitoring changes in the pH of human dental plaque. J Dent Res 1982;61(10):1117–1125. DOI: 10.1177/00220345820610100201
Quiroz EMA, Lingstrom P, Birkhed D. Influence of short-term sucrose exposure on plaque acidogenicity and cariogenicity micro flora in individuals with different levels of Mutans streptococci. Caries Res 2003;37:51–57. DOI: 10.1159/000068221
Devlin TM. Text Book of Biochemistry with Clinical Correlation. 2nd ed. New York: John Wiley and Sons; 1991.
Bowen WH, Pearson SK, Rosalen LP, et al. Assessing the cariogenic potential of some infant formulas, milk and sugar solutions. J Am Dent Assoc 1997;128(7):865–871. DOI: 10.14219/jada.archive.1997.0336
Reynolds EC, Del Rio A. Effect of casein and whey-protein solutions on caries experience and feeding patterns of the rat. Arch Oral Biol 1984;29(11):927–933. DOI: 10.1016/0003-9969(84)90093-1
Peres RC, Coppi LC, Volpato MC, et al. Cariogenic potential of cows’, human and infant formula milks and effect of fluoride supplementation. Br J Nutr 2009;101(3):376–382. DOI: 10.1017/S0007114508020734
