Citation Information :
Shingare P, Chaugule V. An In Vitro Microleakage Study for Comparative Analysis of Two Types of Resin-based Sealants Placed by Using Three Different Types of Techniques of Enamel Preparation. Int J Clin Pediatr Dent 2021; 14 (4):475-481.
Background: Clinicians always experience dilemmas while choosing the type of pit and fissure sealant and the method of enamel preparation before the application of sealant. This study was accomplished using the unfilled and filled types of resin sealant deploying three different techniques of enamel preparation.
Aim and objective: To do a comparative analysis of unfilled and filled sealants by deploying three techniques of enamel preparation.
Materials and methods: The total number of 60 extracted teeth were divided into 3 groups, each containing 20 samples. Conventional acid etching of enamel was labeled as (group I), laser application as (group II), and fissurotomy bur (group III). The samples of group I were prepared by conventional acid etching, the group II was subjected to Er:YAG lasing, while in group III fissurotomy followed by acid etching was done. The sealant placement was done using split tooth design in all the samples. Dye penetration using 5% methylene blue was used for microleakage assessment.
Results: The highest microleakage was found with Gr. II whereas Gr. I exhibited the least microleakage. No statistical difference was observed between the unfilled and filled sealant (p = 0.652).
Conclusion: Conventional acid etching alone or with fissurotomy weighed up appropriate option regardless of the type of sealant material used.
Clinical significance: In regard to the selection of material and proper technique of enamel preparation, this study will be useful to clinicians.
Agarwal A, Shigli A. Comparison of six different methods of cleaning and preparing occlusal fissure surface before placement of pit and fissure sealant: an in vitro study. J Indian Soc Pedod Prev Dent 2012;30(1):51–55. DOI: 10.4103/0970-4388.95582.
Perez-Lajarin L, Cortes-Lillo O, Garcia-Ballesta C, et al. Marginal microleakage of two fissure sealants: a comparative study. J Dentis Child 2003;70(1):24–28.
Fiegal RJ. The use of pit and fissure sealants. Pediatr Dent 2002;24:415–422.
Waggoner WF, Siegal M. Pit and fissure sealant application: updating the technique. J Am Dent Associat 1996;127(3):351–361. DOI: 10.14219/jada.archive.1996.0205.
Droz D, Schiele MJ, Panighi MM. Penetration and microleakage of dental sealants in artificial fissures. J Dentist Child (Chic) 2004;71(1):41–44.
Geiger SB, Gulayev S, Weiss EI. Improving fissure sealant quality: mechanical preparation and filling level. J Dentis 2000;28(6):407–412. DOI: 10.1016/s0300-5712(00)00016-6.
Hatibovic-Kofman S, Wright GZ, Braverman I. Microleakage of sealants after conventional, bur and air abrasion preparation of pits and fissures. Pediat Dentis 1998;20(3):173–176.
Herle GP, Joseph T, Varma B, et al. Comparative evaluation of glass ionomer and resin based fissure sealant using noninvasive and invasive technique-A SEM and microleakage study. J Indian Soc Pedod Prevent Dentis 2004;21(2):56–62.
Mentes A, Gencoglu N. An in vitro study of microleakage of sealants after mechanical or air abrasion techniques with or without acid etching. Eur J Pediatr Dent 2000;1(4):151–156.
Subramanian P, Girish Babu KL, Naveen HK. Effect of tooth preparation on sealant success—an in vitro study. J Clin Pediat Dentis 2009;33(4):325–331. DOI: 10.17796/jcpd.33.4.b276r0374mtx2250.
Duangthip D, Lussi A. Microleakage and penetration ability of resin sealant verses bonding system when applied following contamination. Pediatr Dent 2003;25(5):505–511.
Pardi V, Sinhoreti MA, Pereira AC, et al. In vitro evaluation of microleakage of different materials used as pit and fissure sealants. Braz Dent J 2006;17(1):49–52. DOI: 10.1590/s0103-64402006000100011.
Selecman JB, Owens BM, Johnson WW. Effect of preparation technique, fissure morphology, and material characteristics on the in vitro margin permeability and penetrability of pit and fissure sealants. Pediatr Dent 2007;29(4):308–314.
Dukic W, Dukic OL, Milardovic S. The influence of healozone on microleakage and fissure penetration of different sealing materials. Coll Antropol 2009;33(1):157–162.
Kwom HB, Park KT. SEM and microleakage evaluation of 3 flowable composites as sealants without using bonding agents. Pediatr Dent 2006;28(1):48–53.
Blackwood JA, Dilley DC, Roberts MW, et al. Evaluation of pumice, fissure enameloplasty and air abrasion on sealant microleakage. Pediatr Dent 2002;24(3):199–203.
Koyuturk AE, Kusgoz A, Ulker M, et al. Effects of mechanical and thermal aging on microleakage of different fissure sealants. Dent Mater J 2008;27(6):795–801. DOI: 10.4012/dmj.27.795.
Salama FS, Al-Hammad NS. Marginal seal of sealant and compomer materials with and without enameloplasty. Int J of Pediatr Dent 2002;12(1):39–46. DOI: 10.1046/j.0960-7439.2001.00320.x.
Aguilar FG, Drubi-Filho B, Casemiro LA, et al. Retention and penetration of a conventional resin-based sealant and a photochromatic flowable composite resin placed on occlusal pits and fissures. J Indian Soc Pedod Prev Dent 2007;25(4):169–173. DOI: 10.4103/0970-4388.37012.
Olive G, Genovese MD. Effect of Er: YAG laser parameters on enamel: SEM observations. J Oral Laser Applicat 2007;7(1):27–35.
Khanna R, Pandey RK, Singh N, et al. A comparison of enameloplasty sealant technique with conventional sealant technique: a scanning electron microscope study. J Indian Soc of Pedod and Prev Dent 2009;3(27):158–163. DOI: 10.4103/0970-4388.57096.
Kane B, Karren J, Garcia-Godoy C, et al. Sealant adaptation and penetration into occlusal fissures. Am J Dent 2009;22(2):89–91.
Francescut P, Lussi A. Performance of a conventional sealant and a flowable composite on minimally invasive prepared fissures. Oper Dent 2006;31(5):543–550. DOI: 10.2341/05-91.
Rontani RM, Ducatti CH, Garcia-Godoy F, et al. Effect of etching agent on dentinal adhesive interface in primary teeth. J Clin Pediat Dentis 2000;24(3):205–209.
Overbo RC, Raadal M. Microleakage in fissures sealed with resin or glass Ionomer cement. Scandinav J Dent Res 1990;98:66–69.
Jensen OE, Handleman SL. Effect of an autopolymerizing sealant on viability of microflora in occlusal dental caries. Scandinav J Dent Res 1980;88(5):382–388. DOI: 10.1111/j.1600-0722.1980.tb01243.x.
Meiers JC, Jensen ME. Management of the questionable carious fissure: invasive versus non invasive techniques. J Am Dent Assoc 1984;108(1):64–68. DOI: 10.14219/jada.archive.1984.0203.
Williams B, Von Fraunhofer JA, Winter GB. Microleakage in fissure sealants as determined by dye penetration and zero resistant current measurement studies. Brazel Dent J 1975;139:237–241.
Swartz ML, Phillips RW. In vitro studies on the marginal leakage of restorative materials. J Am Dent Assoc 1961;62(2):141–151. DOI: 10.14219/jada.archive.1961.0021.
Theodoridou-Pahini S, Tolidis K, Papadogiannis Y. Degree of microleakage of some pit and fissure sealants: an in vitro study. Int J Paediatr Dent 1996;6(3):173–176. DOI: 10.1111/j.1365-263x.1996.tb00237.x.
Youssef MN, Youssef FA, Souza-Zaroni WC, et al. Effect of enamel preparation method on in vitro marginal microleakage of a flowable composite used as pit and fissure sealant. Int J Paediatr Dent 2006;16(5):342–347. DOI: 10.1111/j.1365-263X.2006.00751.x.
Ganesh M, Shobha T. Comparative evaluation of the marginal sealing ability of fuji VII and concise as pit and fissure sealants. J Contemp Dent Pract 2007;8(4):1–8. DOI: 10.5005/jcdp-8-4-10.
Borsatto MC, Corona SA, Dibb RJ, et al. Microleakage of a resin sealant after acid etching, Er:YAG laser irradiation and air abrasion of pits and fissures. J Clin Laser Med Surg 2001;19(2):83–87. DOI: 10.1089/104454701750285403.
Manhart J, Huth KC, Chen HY, et al. Influence of the pretreatment of occlusal pits and fissures on the retention of a fissure sealant. Am J Dentis 2004;17(1):12–18.
Grande RH, Ballester R, Singer Jda M, et al. Microleakage of a universal adhesive used as a fissure sealant. Am J Dentis 1998;11(3):109–113.
Boj JR, Xalabrade A, Garcia-Godoy F. Microleakage of fissure sealants after enameloplasty. Pediat Dentis 1995;17:143. (abstract).
Hatibovic-Kofman S, Butler SA, Sadek H. Microleakage of three sealants following conventional, bur, and air-abrasion preparation of pit and fissures. Int J Pediat Dentis 2001;11(6):409–416. DOI: 10.1046/j.0960-7439.2001.00303.x.
Xalabarde A, Garcia-Godoy F, Boj JR, et al. Microleakage of fissure sealants after occlusal enameloplasty and thermocycling. J Clin Pediat Dentis 1998;22(3):231–235.
Algarni M, Elkwatehy W. Microleakage of different pit and fissure sealants. Int J Dentis Oral Sci 2017;4(10):532–536.
Halterman CW, Rayman R, Rabbach V. Survey of pediatric dentists concerning dental sealants. Pediat Dentis 1995;17(7):455–456.
Borsatto MC, Corona SA, Ramos RP, et al. Microleakage at sealant/enamel interface of primary teeth: effect of Er:YAG laser ablation of pits and fissures. J Dent Child (Chic) 2004;71(2):143–147.
do Rego MA, de Araujo MA. Microleakage evaluation of pit and fissure sealants done with different procedures, materials, and laser after invasive technique. J Clin Pediat Dentis 1999;24(1):63–68.
Lupi-Pégurier L, Bertrand MF, Genovese O, et al. Microleakage of resin-based sealants after Er:YAG laser conditioning. Lasers Med Sci 2007;22(3):183–188. DOI: 10.1007/s10103-006-0437-3.
Corona SAM, Borsatto MC, Pecora JD, et al. Assessing microleakage of different class V restorations after Er: YAG laser and bur preparation. J Oral Rehabilitat 2003;30(10):1008–1014. DOI: 10.1046/j.1365-2842.2003.01173.x.
Topaloglu-Ak A, Onçag O, Gökçe B, et al. The effect of different enamel surface treatments on microleakage of fissure sealants. Acta Med Acad 2013;42(2):223–228. DOI: 10.5644/ama2006-124.90.
Moshonov J, Stabholz A, Zyskind D, et al. Acid etched and Erbium:Yttrium Aluminium garnet laser treated enamel for fissure sealants: a comparison of microleakage. Int J Pediatr Dent 2005;15(3):205–209. DOI: 10.1111/j.1365-263X.2005.00624.x.
Dostalova T, Jelinkova H, Krejsa O, et al. Er:YAG laser etching of enamel. Scanning Microsc 1998;12(2):309–315.
Park K, Georgescu M, Scherer W, et al. Comparison of shear strength, fracture patterns, and microleakage among unfilled, filled, and fluoride-releasing sealants. Pediatr Dent 1993;15(6):418–421.
Xalabarde A, Garcia-Godoy F, Boj JR, et al. Fissure micromorphology and sealant adaptation after occlusal enameloplasty. J Clin Pediat Dentis 1996;20(4):299–304.