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VOLUME 14 , ISSUE 2 ( March-April, 2021 ) > List of Articles

RESEARCH ARTICLE

Antimicrobial Efficacy of Triple Antibiotic Paste and Propolis as an Intracanal Medicament in Young Permanent Teeth: An In Vivo Study

Edara Lillygrace, Balaji Kethineni, Hemanth KL Raichurkar, Srilekha Ambati, Kanamarlapudi V Saikiran

Keywords : Propolis, Sodium hypochlorite, Triple antibiotic paste, Young permanent teeth

Citation Information : Lillygrace E, Kethineni B, Raichurkar HK, Ambati S, Saikiran KV. Antimicrobial Efficacy of Triple Antibiotic Paste and Propolis as an Intracanal Medicament in Young Permanent Teeth: An In Vivo Study. Int J Clin Pediatr Dent 2021; 14 (2):243-248.

DOI: 10.5005/jp-journals-10005-1944

License: CC BY-NC 4.0

Published Online: 30-07-2021

Copyright Statement:  Copyright © 2021; The Author(s).


Abstract

Aim and objective: To evaluate the antimicrobial efficacy of triple antibiotic paste and propolis extracts as an intracanal medicament in young permanent teeth. Materials and methods: A total of 30 single-rooted non-vital young permanent teeth with open apex were selected randomly from the children aged between 7 years and 14 years with no systemic complications. Group I—triple antibiotic paste and group II be propolis allocating 15 teeth in each group. After access opening, the first sample (S1) was collected by inserting paper point into the root canal, the second sample (S2) was collected immediately after irrigation, and the third sample (S3) was collected after post-intracanal medication after 3–4 weeks. Samples were sent for microbiological analysis to assess the bacterial count, and for the obtained data, statistical analysis was done. Results: The mean colony count among the triple antibiotic paste group was 1906.75. After access opening, which was reduced to 315.12 after irrigation, and after 3–4 weeks, it was 817.25. There was a significant difference between sample 1, sample 2, and sample 3 (p value = 0.008). The mean colony count among the propolis group was 1427.87 after access opening, which was reduced to 436.00 after irrigation, and after 3–4 weeks, it has reduced to 252.37. There was a significant difference between sample 1, sample 2, and sample 3 (p value = 0.032). Intergroup comparison between the groups showed no statistical difference between the samples. Conclusion: Propolis exhibited similar antimicrobial efficacy, which is comparable to triple antibiotic paste. So, propolis can be utilized as an intracanal medicament in young permanent teeth with an open apex. Clinical significance: Propolis is a naturally occurring flavonoid-rich resinous product with antibacterial, antifungal, antiviral, immunomodulatory, and antioxidant effects. It is safe without any drug allergies and bacterial sensitivity and is a promising alternative to triple antibiotic paste for disinfecting non-vital young permanent teeth.

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  1. Mente J, Hage N, Pfefferle T, et al. Mineral trioxide aggregate apical plugs in teeth with open apical foramina: a retrospective analysis of treatment outcome. J Endod 2009;35(10):1354–1358. DOI: 10.1016/j.joen.2009.05.025.
  2. Sheehy EC, Roberts GJ. Use of calcium hydroxide for apical barrier formation and healing in non-vital immature permanent teeth: a review. Br Dent J 1997;183(7):241–246. DOI: 10.1038/sj.bdj.4809477.
  3. Huang GT. Apexification: the beginning of its end. Int Endod J 2009;42(10):855–866. DOI: 10.1111/j.1365-2591.2009.01577.x.
  4. Hoshino E, Kurihara-Ando N, Sato I, et al. In-vitro antibacterial susceptibility of bacteria taken from infected root dentine to a mixture of ciprofloxacin, metronidazole and minocycline. Int Endod J 1996;29(2):125–130. DOI: 10.1111/j.1365-2591.1996.tb01173.x.
  5. Bose R, Nummikoski P, Hargreaves K. A retrospective evaluation of radiographic outcomes in immature teeth with necrotic root canal systems treated with regenerative endodontic procedures. J Endod 2009;35(10):1343–1349. DOI: 10.1016/j.joen.2009.06.021.
  6. Jong HK, Yuram K, Shei SJ, et al. Tooth discoloration of immature permanent incisor associated with triantibiotic paste therapy. A case report. J Endod 2010;36(6):1086–1091. DOI: 10.1016/j.joen.2010.03.031.
  7. Ikeno K, Ikeno T, Miyazawa C. Effect of propolis on dental caries in rats. Caries Res 1991;25(5):347–351. DOI: 10.1159/000261390.
  8. Margaret P, Martin, Pileggi R. A quantitative analysis of propolis; a promising new storage media following avulsion. Dent Traumatol 2004;20(2):85–89. DOI: 10.1111/j.1600-4469.2004.00233.x.
  9. Gopikrishna V, Parminder S, Bameja, et al. Comparison of coconut water, propolis, HBSS on PDL cell survival. J Endod 2008;34(5):587–589. DOI: 10.1016/j.joen.2008.01.018.
  10. Sabir A, Tabbu CR, Agustina P, et al. Histological analysis of rat dental pulp tissue capped with propolis. J Oral Sci 2005;47(3):135–138. DOI: 10.2334/josnusd.47.135.
  11. Almas K, Mahmoud A, Dehla A. A comparative study of propolis and saline application on human dentin. A SEM study. Indian J Dent Res 2001;12(1):21–27.
  12. Al Shaheer A, Wallace J, Agarwal S, et al. Effect of propolis on human fibroblast from the dental pulp and periodontal ligament. J Endod 2004;30(5):359–361. DOI: 10.1097/00004770-200405000-00012.
  13. Bankora VS, Propov SS, Marekov NL. A study of flavanoid of propolis. J Nat Prod 1983;46(4):471–474. DOI: 10.1021/np50028a007.
  14. Kandaswamy D, Venkateshbabu N, Gogulnath D, et al. Dentinal tubule disinfection with 2% chlorhexidine gel, propolis, morinda citrofolia juice, 2% povidone iodine and calcium hydroxide. Int Endod J 2010;43(5):419–423. DOI: 10.1111/j.1365-2591.2010.01696.x.
  15. Awawdeh L, Bertarm AL, Hammad M. Effectiveness of propolis and calcium hydroxide as a short term intracanal medicament against Enterococcus faecalis: a laboratory study. Aust Endod J 2009;35(2):52–58. DOI: 10.1111/j.1747-4477.2008.00125.x.
  16. Torabinejad M, Chivian N. Clinical applications of mineral trioxide aggregate. J Endod 1999;25(3):197–205. DOI: 10.1016/S0099-2399(99)80142-3.
  17. Becerra P, Ricucci D, Loghin S, et al. Histological study of a human immature permanent premolar with chronic apical abscess after revascularisation/revitalisation. J Endod 2014;40(1):133–139. DOI: 10.1016/j.joen.2013.07.017.
  18. Torneck CD, Smith JS, Grindall P. Biologic effects of endodontic procedures on developing incisor teeth: II - effect of pulp injury and oral contamination. Oral Surg Oral Med Oral Pathol 1973;35(3):378–388. DOI: 10.1016/0030-4220(73)90076-5.
  19. Mohammad Z. Sodium hypochlorite in endodontics: an update review. Int Dent J 2008;58(6):329–341. DOI: 10.1111/j.1875-595X.2008.tb00354.x.
  20. Kontakiotis EG, Filippatos CG, Tzanetakis GN, et al. Regenerative endodontic therapy: a data analysis of clinical protocols. J Endod 2015;41(2):146–154. DOI: 10.1016/j.joen.2014.08.003.
  21. Trevino EG, Patwardhan AN, Henry MA, et al. Effect of irrigants on the survival of human stem cells of the apical papilla in a platelet-rich plasma scaffold in human root tips. J Endod 2011;37(8):1109–1115. DOI: 10.1016/j.joen.2011.05.013.
  22. Kalhoro FA, Anwar K, Shaikh MA, et al. Rate of apical extrusion of sodium hypochlorite: open ended versus closed ended needles. Pak Oral Dental J 2014;34(1):159–163.
  23. Chen MH, Chen KL, Chen CA, et al. Responses of immature permanent teeth with infected necrotic pulp tissue and apical periodontitis/abscess to revascularisation procedures. Int Endod J 2012;45(3):294–305. DOI: 10.1111/j.1365-2591.2011.01978.x.
  24. El Ashiry EA, Farsi NM, Abuzeid ST, et al. Dental pulp revascularisation of necrotic permanent teeth with immature apices. J Clin Pediatr Dent 2016;40(5):361–366. DOI: 10.17796/1053-4628-40.5.361.
  25. Sato I, Ando-Kurihara N, Kota K, et al. Sterilization of infected root-canal dentine by topical application of a mixture of ciprofloxacin, metronidazole and minocycline in situ. Int Endod J 1996;29(2):118–124. DOI: 10.1111/j.1365-2591.1996.tb01172.x.
  26. Ruparel NB, Teixeira FB, Ferraz CC, et al. Direct effect of intracanal medicaments on survival of stem cells of the apical papilla. J Endod 2012;38(10):1372–1375. DOI: 10.1016/j.joen.2012.06.018.
  27. Kousedghi H, Ahangari Z, Eslami G, et al. Antibacterial activity of propolis and Ca(OH)2 against Lactobacillus, Enterococcus faecalis, Peptostreptococcus and Candida albicans. Afr J Microbiol Res 2012;6(14):3510–3515. DOI: 10.5897/AJMR12.029.
  28. Bankova V, Christov R, Kujumgiev A, et al. Chemical composition and antibacterial activity of Brazilian propolis. Z Naturforsch C J Biosci 1995;50(3-4):167–172. DOI: 10.1515/znc-1995-3-402.
  29. Al-Qathami H, Al-Madi E. Comparison of sodium hypochlorite, propolis and saline as root canal irrigants: a pilot study. Saudi Dent J 2003;15(2):100–103.
  30. Rouhani A, Erfanzadeh M, Jafarzadeh H, et al. Comparison of residual triple antibiotic paste, propolis and calcium hydroxide on root canal walls in natural open apex teeth: an in vitro study. Iran Endod J 2018;13(1):25–29. DOI: 10.22037/iej.v13i1.15807.
  31. Pagliarin CM, Londero CD, Felippe MC, et al. Tissue characterisation following revascularisation of immature dog teeth using different disinfection pastes. Braz Oral Res 2016;30(1):e74. DOI: 10.1590/1807-3107BOR-2016.vol30.0074.
  32. El-Tayeb MM, Abu-Seida AM, El Ashry SH, et al. Evaluation of antibacterial activity of propolis on regenerative potential of necrotic immature permanent teeth in dogs. BMC Oral Health 2019;19(1):174. DOI: 10.1186/s12903-019-0835-0.
  33. Parolia A, Kundabala M. Nonsurgical retreatment of failed surgical endodoSntic therapy using propolis as an intra-canal medicament: a case report. Res J Med Sci 2010;4(4):292–297. DOI: 10.3923/rjmsci.2010.292.297.
  34. Ding RY, Cheung GS, Chen J, et al. Pulp revascularisation of immature teeth with apical periodontitis: a clinical study. J Endod 2009;35(5):745–749. DOI: 10.1016/j.joen.2009.02.009.
  35. Windley W, Teixeira F, Levin L, et al. Disinfection of immature teeth with a triple antibiotic paste. J Endod 2005;31(6):439–443. DOI: 10.1097/01.don.0000148143.80283.ea.
  36. Asgary S, Fazlyab M, Nosrat A. Regenerative endodontic treatment versus apical plug in immature teeth: three-year follow-up,. J Clin Pediatr Dent 2016;40(5):356–360. DOI: 10.17796/1053-4628- 40.5.356.
  37. Neelamurthy PS, Kumar RA, Balakrishnan V, et al. Revascularisation in immature and mature teeth with necrotic pulp: a clinical study. J Contemp Dent Pract 2018;19(11):1393–1399. DOI: 10.5005/jp-journals-10024-2438.
  38. Chua EG, Parolia A, Ahlawat P, et al. Antifungal effectiveness of various intra canal medicaments against Candida albicans: an ex-vivo study. BMC Oral Health 2014;14(1):1–8. DOI: 10.1186/1472-6831-14-53.
  39. Sabharwal S, Bhagat SK, Gami KS, et al. An in vivo study to compare antimicrobial activity of triantibiotic paste, 2% chlorhexidine gel, and calcium hydroxide on microorganisms in the root canal of immature teeth. J Int Soc Prev Community Dent 2019;9(3):263–268. DOI: 10.4103/jispcd.JISPCD_400_18.
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