International Journal of Clinical Pediatric Dentistry

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VOLUME 14 , ISSUE 2 ( March-April, 2021 ) > List of Articles


Estimation of Copper Levels in Saliva and Its Relation to Dental Caries and Hemoglobin Levels

Vundela Rajashekar Reddy, Shruthi Devakar, Sakleshpura Mruthyunjaya Chaitan, Ravigna Peddi, Paluvary Sharath Kumar

Keywords : Caries, Copper levels, Hemoglobin, Saliva

Citation Information : Reddy VR, Devakar S, Chaitan SM, Peddi R, Kumar PS. Estimation of Copper Levels in Saliva and Its Relation to Dental Caries and Hemoglobin Levels. Int J Clin Pediatr Dent 2021; 14 (2):235-237.

DOI: 10.5005/jp-journals-10005-1938

License: CC BY-NC 4.0

Published Online: 30-07-2021

Copyright Statement:  Copyright © 2021; The Author(s).


Context: A very small quantity of trace elements will be present in saliva. Salivary composition influences caries inhibition and promotion based on the different organic, inorganic, and trace elements. Aim and objective: To evaluate the levels of copper in saliva in children with caries-active and caries-free group and to associate copper levels of saliva with hemoglobin (Hb) levels of blood. Materials and methods: Sixty subjects between the ages of 6 and 14 years were divided into group I—caries-free and group II—caries-active. The caries-active group was further subdivided into group IIa (deft/DMFT < 3) and group IIb (deft/DMFT > 3). To maintain the uniformity of salivary composition, unstimulated saliva (5 mL) was collected in noontime before food from all the participants. The copper levels were estimated by atomic absorption spectrophotometry. Hemoglobin levels in the blood and their relation with copper levels in saliva were estimated. Statistical analysis: The results thus obtained were analyzed by t-test and Karl Pearson's correlation coefficient. It was considered significant if the “p” value is 0.05 or less. Results: Copper levels were higher in group II showing statistically highly significant results compared with that of group I with p ≤ 0.00001. The results were no significant with respect to Hb scores among the groups. Conclusion: A definite positive correlation is seen with dental caries and levels of copper in saliva. Hence, the amount of copper in saliva can be considered as a caries risk assessment tool.

  1. Sejdini M, Begzati A, Salihu S, et al. The role and impact of salivary Zn levels on dental caries. Int J Dent 2018;2018:8137915. DOI: 10.1155/2018/8137915.
  2. Hussein AS, Ghasheer HF, Ramli NM, et al. Salivary trace elements in relation to dental caries in a group of muiti-ethnic schoolchildren in shah Alam, Malaysia. Eur J Paediatr Dent 2013;14(2):113–118. DOI: 10.1007/s40368-013-0018-7.
  3. Sekhri P, Sandhu M, Sachdev V, et al. Estimation of trace elements in mixed saliva of caries-free and caries active children. J Clin Pediatr Dent 2018;42(2):135–139. DOI: 10.17796/1053-4628-42.2.9.
  4. Duggal M, Chawla H, Curzon M. A study of the relationship between trace element in saliva and dental caries in children. Arch of Oral Biol 1991;36(12):881–884. DOI: 10.1016/0003-9969(91)90118-e.
  5. Watanabe K, Tanaka T, Shigemi T, et al. Mn and Cu concentrations in mixed saliva of elementary school children in relation to sex, age and dental caries. J Trace Elem Med Bio 2009;23(2):93–99. DOI: 10.1016/j.jtemb.2009.01.003.
  6. Hegde MN, Hegde ND, Ashok A, et al. Biochemical indicators of dental caries in saliva: an in vivo study. Caries Res 2014;48(2):170–173. DOI: 10.1159/000355580.
  7. Daryani KK, Poonacha KS, Deshpande AN, et al. Comparative evaluation of serum iron level, serum ferritin level and salivary pH with dental caries in children with iron deficiency anaemia - an observational cross-sectional study. J Adv Med Dent Sci Res 2019;7(4):16–18.
  8. Hashemi A, Bahrololoomi Z, Salarian S. Relationship between early childhood caries and anemia: a systematic review. Iran J Ped Hematol Oncol 2018;8(2):126–138.
  9. Bhattacharya PT, Misra SR, Hussain M. Nutritional aspects of essential trace elements in oral health and disease; an extensive review. Scientifica (Cairo) 2016;2016:5464373. DOI: 10.1155/2016/5464373.
  10. Hedge MN, Attavar SH, Shetty N, et al. Saliva as a biomarker for dental caries: a systemic review. J Conserv Dent 2019;22:2–6.
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