International Journal of Clinical Pediatric Dentistry

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VOLUME 14 , ISSUE 1 ( January-February, 2021 ) > List of Articles

RESEARCH ARTICLE

Comparative Evaluation of Efficacy of Kefir Milk Probiotic Curd and Probiotic Drink on Streptococcus mutans in 8–12-year-old Children: An In Vivo Study

Saida Reddy, Vasepalli Madhu, Rachuri Punithavathy, Martha Satyam, Uday Kumar Chowdary, Raparla Mythraiye

Keywords : ANOVA, Kefir milk, Post hoc, Probiotic curd, Probiotic drink, Streptococcus mutans

Citation Information : Reddy S, Madhu V, Punithavathy R, Satyam M, Chowdary UK, Mythraiye R. Comparative Evaluation of Efficacy of Kefir Milk Probiotic Curd and Probiotic Drink on Streptococcus mutans in 8–12-year-old Children: An In Vivo Study. Int J Clin Pediatr Dent 2021; 14 (1):120-127.

DOI: 10.5005/jp-journals-10005-1883

License: CC BY-NC 4.0

Published Online: 14-07-2021

Copyright Statement:  Copyright © 2021; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

Aim and objective: To compare the effect of probiotic products in reducing the levels of salivary Streptococcus mutans before and after their consumption. Materials and methods: Eighty school children with initial carious lesions in the age group of 8–12 years were selected and divided into four groups. Children in group I (control) were undergone with restorations without supplementation of probiotics. Children in group II (kefir milk), group III (probiotic curd), and group IV (probiotic drink) supplemented 100 mL of their respective probiotics for 1 month. Assessment of saliva sample was done at baseline, 1 hour after administration of probiotics followed by weekly intervals till 1 month. Results: The study showed a marked reduction in colony-forming units (CFUs) at a 1-hour time interval in all four groups when compared to baseline. On the 30th-day, children in group II and group III have shown an equal reduction of CFUs when compared to group IV and group I. Conclusion: Probiotic products like kefir milk and probiotic curd have shown an equal and marked decrease in CFUs when compared to the probiotic drink group. Clinical significance: The administration of probiotics along with dairy products can be used as an adjuvant to routine preventive treatment procedures in the prevention of dental caries along with the remineralization of the demineralized tooth structure.


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  1. Patidar D, Sogi S, Singh V, et al. Salivary levels of Streptococcus mutans and Streptococcus sanguinis in early childhood caries: An in vivo study. J Indian Soc Pedod Prev Dent 2018;36:386–390.
  2. Kundu H, Basavaraj P, Ashish S, et al. Dental caries scenario among 5, 12 and 15-year-old children in India - a retrospective analysis. J Clin Diagnos Res 2015;9(7):ZE01–ZE05.
  3. Caglar E, Kargul B, Tanboga I. Bacteriotherapy and probiotics’ role on oral health. Oral Dis 2005;11:131–137.
  4. Srivastava S, Saha S, Kumari M, et al. Effect of probiotic curd on salivary pH and Streptococcus mutans: A double-blind parallel randomized controlled trial. JCDR 2016;10(2):13–16.
  5. Lee SH, Kim YJ. A comparative study of the effect of probiotics on cariogenic biofilm model for preventing dental caries. Arch Microbiol 2014;196(8):601–609.
  6. Guzel-Seydim ZB, Kok-Tas T, Greene AK, et al. Review: functional properties of kefir. Crit Rev Food Sci Nutrit 2011;51(3):261–268.
  7. Yousuf A, Nagaraj A, Shravani G, et al. Comparative evaluation of commercially available freeze-dried powdered probiotics on mutans streptococci count. A randomized, double-blind, clinical study. JDT Tums 2015;12(10):729–738.
  8. Xiolong L, Chen XI, Yan T, et al. Effect of probiotic lactobacilli on the growth of Streptococcus mutans and multispecies biofilms isolated from children with active caries. Med Sci Monit 2017;2017(23): 4175–4181.
  9. Kim Y-J, Lee S-H. Inhibitory effect of Lactococcus lactis HY 449 on cariogenic biofilm. J Microbiol Biotechnol 2016;26(11):1829–1835.
  10. Reddy KS, Reddy S, Ravindhar P, et al. Prevalence of dental caries among 6–12 years school children of Mahbubnagar District, Telangana State, India: A cross-sectional study. Indian J Dent Sci 2017;9:1–7.
  11. Prado MR, Blandón LM, Vandenberghe LPS, et al. Milk kefir: composition, microbial cultures, biological activities, and relatedproducts. Front Microbiol 2015;6:1177.
  12. Farnworth ER. Kefir-a complex probiotic. Food Sci Technol Bull: Funct Foods 2005;2(1):1–17.
  13. Rodrigues KL, Caputo LRG, Carvalho JCT, et al. Antimicrobial and healing activity of kefir and kefiran extract. Int J Antimicro Agents 2005;25(5):404–408.
  14. Cogulu D, Topaloglu-Ak A, Caglar E, et al. Potential effects of a multistrain probiotic-kefir on salivary Streptococcus mutans and Lactobacillus spp. J Dent Sci 2010;5(3):144–149.
  15. Yadav M, Poornima P, Roshan N, et al. Evaluation of probiotic milk on salivary mutans streptococci count: an in vivo microbiological study. J Clin Pediat Dentis 2014;39(1):23–26.
  16. Gold OG, Jordan HV, van Houte J. A selective medium for Streptococcus mutans. Archi Oral Biol 1973;18(11):1357–1364.
  17. ANKER HS, JOHNSON BA. Bacitracin; methods of production, concentration, and partial purification, with a summary of the chemical properties of crude bacitracin. J Bacteriol 1948;55(2):249–255.
  18. Lima LM, Motisuki C, Spolidorio DM, et al. In vitro evaluation of probiotics microorganisms’ adhesion to an artificial caries model. Eur J Clin Nutr 2005;59:884–886.
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