Citation Information :
Jagannathan J, Nagar P, Kaniappan AS, Raveendran A, Shekhar S. Comparison of Antimicrobial Efficacy of Natural Extracts as a Disinfectant for Removable Orthodontic Appliances: An Ex Vivo Study. Int J Clin Pediatr Dent 2020; 13 (6):640-643.
Aim: The purpose of the study is to formulate a standard protocol for cleaning and disinfecting removable appliances by evaluating the antimicrobial efficacy of three natural extracts.
Materials and methods: Fifty patients with removable orthodontic appliances were selected and divided into five groups [chlorhexidine 0.2% (positive control), extracts of neem, katha, cinnamon, and normal saline (negative control)] with 10 samples each. Saliva sample from the appliance was collected using a sterile cotton swab and cultured. Agar diffusion method was used and zones of inhibition were measured.
Statistical analysis: The obtained data were subjected to statistical analysis by the Kruskal–Wallis ANOVA test and the Mann–Whitney U test.
Results: The neem extract had the highest zone of inhibition measuring 14.95 mm (mean) followed by katha extract with the value of 13.85 mm which was found to be comparable to chlorhexidine with 15.85 mm.
Conclusion: Thus, neem and katha extracts can be used to disinfect removable appliances because of their good antimicrobial activity.
Clinical significance: By following these simple cleaning protocols, it is possible to avoid numerous health hazards caused by unclean removable appliances in children.
Semenoff TDV, Semenoff-Segundo A, Henrique Borges A, et al. Antimicrobial activity of 2% chlorhexidine gluconate, 1% sodium hypochlorite and paramonochlorophenol combined with furacin against S. aureus, C. albicans, E. faecalis and P. aureginosa. Rev Odonto ciênc 2010;25(2):174–177.
Mathur S, Mathur T, Srivastava R, et al. Chlorhexidine: the gold standard in chemical plaque control. Nat J Physiol, Pharm Pharmacol 2011;1(2):45–50.
Francine U, Jeannette U, Pierre. RJ. Assessment of antibacterial activity of neem plant (Azadirachta indica) on Staphylococcus aureus and Escherichia coli. J Med Plants Stud 2015;3(4):85–91.
Parashar G, Sutar N, Sanap S. Antibacterial activity of mixture of leaf extracts of neem (Azadirachta indica Linn.) and Tantani (Lantana camara) Int J Pharm Sci Res 2018;9(6):2545–2549.
Krishnan Y, Wong NK. Cytotoxicity and antimicrobial properties of neem (Azadirachta indica) leaf extracts. Int J Pharm Sci 2015;7(2):179–182.
Hegde V, Kesaria DP. Comparative evaluation of antimicrobial activity of neem, propolis, turmeric, liquorice and sodium hypochlorite as root canal irrigants against E. faecalis and C. albicans - an in vitro study. J Endodontol 2013;25(2):38–45.
Ghonmode WN, Balsaraf OD, Tambe VH, et al. Comparison of the antibacterial efficiency of neem leaf extracts, grape seed extracts and 3% sodium hypochlorite against E. feacalis – an in vitro study. J Int Oral Health 2013;5(6):61–66.
Biswas G, Anup N, Acharya S, et al. Evaluation of the efficacy of 0.2% chlorhexidine versus herbal oral rinse on plaque induced gingivitis - a randomized clinical trail. IOSR J Nurs Health Sc 2014;3(2V):58–63. DOI: 10.9790/1959-03255863.
Lakshmi T, Geetha RV, Roy A. In vitro antibacterial activity of ethanolic bark extract of acacia catechu willd against enteric pathogens. Int J Drug Dev Res 2011;3(3):328–334.
Waty S, Suryanto D, Yurnaliza. Antibacterial activity of cinnamon ethanol extract (Cinnamomum burmannii) and its application as a mouthwash to inhibit streptococcus growth. IOP Conf. Series: Earth and Environmental Science 130 (2018) 012049.
Souza Lamas RR, Suca Salas MM, Cenci TP, et al. Removable orthodontic appliances: frequency and cleaning agents used by students and recommended by dentists. Braz J Oral Sci 2016;15(1):21–26. DOI: 10.20396/bjos.v15i1.8647093.