International Journal of Clinical Pediatric Dentistry

Register      Login

VOLUME 13 , ISSUE 2 ( March-April, 2020 ) > List of Articles

Original Article

Comparative Evaluation of Antifungal Efficacy of Five Root Canal Sealers against Clinical Isolates of Candida albicans: A Microbiological Study

Sameep Singh, Binita Srivastava, Khyati Gupta, Rashi Singh, Satyavir Singh

Keywords : Agar-well diffusion, Candida albicans, Endomethasone, Metapex, MTA Fillapex, Root canal sealers, Tube dilution test, Zinc oxide eugenol

Citation Information : Singh S, Srivastava B, Gupta K, Singh R, Singh S. Comparative Evaluation of Antifungal Efficacy of Five Root Canal Sealers against Clinical Isolates of Candida albicans: A Microbiological Study. Int J Clin Pediatr Dent 2020; 13 (2):119-123.

DOI: 10.5005/jp-journals-10005-1718

License: CC BY-NC 4.0

Published Online: 23-07-2020

Copyright Statement:  Copyright © 2020; The Author(s).


Abstract

Aim and objective: The aim of this study was to evaluate and compare the antifungal efficacy of MTA Fillapex, Metapex, zinc oxide eugenol cement, Endomethasone, and Endoflas against Candida albicans. Materials and methods: Root canal exudates of 30 patients were tested against MTA Fillapex (Angelus), Metapex (BioMed), zinc oxide eugenol (Deepak Enterprise), Endomethasone (Septodont), Endoflas FS (Sanlor Laboratories), MTA (Angelus) (positive control), and glycerine (negative control). Children with failed endodontic cases were included in the study. Tube dilution and agar diffusion methods were used to check the antifungal efficacy of the root canal sealers. In tube dilution method, 24-well culture plates containing freshly mixed material along with Candida albicans were used. Wells containing MTA (Angelus) along with Sabouraud dextrose agar and Candida albicans served as positive control while glycerine along with Sabouraud dextrose agar and Candida albicans served as negative control. All plates were incubated at 37°C for 24 hours. Growth of the fungi was monitored after 24 hours by the presence of the turbidity. The samples were recultured to test the experimental material using agar well diffusion method, and the Petri plates were incubated for 24 hours and 72 hours. Zone of inhibition was measured after respective time period. Paired t test was used for the data analysis. Results: It was seen in tube dilution method Endomethasone showed least turbidity while maximum was shown by Metapex; similar results were seen in case of agar well diffusion method in which largest zone of inhibition was shown by Endomethasone while smallest was by Metapex. Conclusion: It was concluded that Endomethasone showed maximum efficacy against Candida albicans as compared to Metapex.


HTML PDF Share
  1. Kangarlou A, Sofiabadi S, Yadegari Z, et al. Antifungal effect of calcium enriched mixture cement against Candida albicans. Int Endod J 2009;4(3):101–105.
  2. Baumgartner JC, Watts CM, Xia T. Occurence of Candida albicans in infections of endodontic origin. J Endod 2000;26(12):695–698. DOI: 10.1097/00004770-200012000-00003.
  3. Waltimo TM, Ørstavik D, Meurman JH, et al. In vitro susceptibility of Candida albicans isolates from and marginal periodontitis to common antifungal agents. Oral Microbial Immunol 2000;15(4):245–248. DOI: 10.1034/j.1399-302x.2000.150406.x.
  4. Mohammadi Z, Modaresi J, Mohammad Y. Evaluation of the antifungal effects of mineral trioxide aggregate materials. Aust Endod J 2006;32(3):120–122. DOI: 10.1111/j.1747-4477.2006.00032.x.
  5. Amorim LDFGD, Toledo OAD, Estrela CRDA, et al. Antimicrobial analysis of different root canal filling pastes used in pediatric dentistry by two experimental methods. Braz Dent J 2006;17(4):317–322. DOI: 10.1590/S0103-64402006000400010.
  6. Drucker DB, Natsiou I. Microbial ecology of the dental root canal. Microb Ecol Health Dis 2000;12(3):160–169. DOI: 10.1080/089106000750051837.
  7. Sequeira Jr JF, Sen BH. Fungi in endodontic infection. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2004;97(5):632–641. DOI: 10.1016/j.tripleo.2003.12.022.
  8. Kaiwar A, Nadig G, Hegde J, et al. Assessment of antimicrobial activity of endodontic sealers of Enterococcus faecalis: an in vitro study. World J Dent 2012;3(1):26–31. DOI: 10.5005/jp-journals-10015-1123.
  9. Cassanho ASA, Fernandes AM, Oliveira LD, et al. In vitro activity of zinc oxide eugenol and glass ionomer cements on Candida albicans. Braz Oral Res 2005;19(2):134–138. DOI: 10.1590/S1806-83242005000200011.
  10. Poggio C, Lombardini M, Colombo M, et al. Antibacterial effects of six endodontics sealers. Int J Artif Organs 2011;34(9):908–913. DOI: 10.5301/ijao.5000055.
  11. Mohammadi Z, Khademi AA, Ardakani FE. In vitro evaluation of antifungal effects of Mineral trioxide aggregate and Portland cement on Candida albicans. Int Endod J 2006;1(4):137–140.
  12. Badr AE, Razek AAA, Omar NS, et al. Evaluation of the antimicrobial and cytotoxic activity of epiphany root canal sealer in vitro Egyptian. J Med Microbiol 2007;16(1):95–104.
  13. de Queiroz AM, Nelson-Filho P, da Silva LAB, et al. Antibacterial activity of root canal filling materials for primary teeth: zinc oxide and eugenol cement, calen paste thickened with zinc oxide, Sealapex and Endorez. Braz Dent J 2009;20(4):290–296. DOI: 10.1590/S0103-64402009000400005.
  14. Saha S, Samadi F, Jaiswal JN, et al. Antimicrobial activity of different endodontic sealers: an in vitro evaluation. J Indian Soc Pedod Prev Dent 2010;28(4):251–257. DOI: 10.4103/0970-4388.76151.
  15. Gomes BPFDE, Pedroso JA, Jacinto RC, et al. In vitro evaluation of the antimicrobial activity of five root canal sealers. Braz Dent J 2004;15(1):30–35. DOI: 10.1590/S0103-64402004000100006.
  16. Reyhani MF, Ghasemi N, Milani AS, et al. Antimicrobial effects of apexit plus, epiphany, MTA Fillapex and dorifill sealers on enterococcus faecalis at different time intervals. Eur Int J Sci Human 2015;2(4):10–15.
  17. Scientific profile: MTA Fillapex endodontic sealer (Angelus).
  18. Harini Priya M, Bhat SS, Sandeep Hegde K. Comparative evaluation of bactericidal potential of four root canal filling material against microflora of infected non-vital primary teeth. J Clin Pediatr Dent 2010;35(1):23–29. DOI: 10.17796/jcpd.35.1.u57p4500360g2752.
  19. Bystrom A, Claesson R, Sundqvist G. The antibacterial effect of camphorated paramonochlorophenol, camphorated phenol and calcium hydroxide in the treatment of infected root canals. Endo Dent Traumatol 1985;1(5):170–175. DOI: 10.1111/j.1600-9657.1985.tb00652.x.
PDF Share
PDF Share

© Jaypee Brothers Medical Publishers (P) LTD.