International Journal of Clinical Pediatric Dentistry

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VOLUME 12 , ISSUE 2 ( March-April, 2019 ) > List of Articles

Original Article

Testing the Clinical Applicability of Resin Infiltration of Developmental Enamel Hypomineralization Lesions Using an In Vitro Model

Dagmar Schnabl, Viktoria Dudasne-Orosz, Rudolf Glueckert, Stephan Handschuh, Ines Kapferer-Seebacher, Herbert Dumfahrt

Keywords : Cheese molars, Developmental enamel hypomineralization, Laboratory research, Low-viscosity resin, Penetration depth, Resin infiltration

Citation Information : Schnabl D, Dudasne-Orosz V, Glueckert R, Handschuh S, Kapferer-Seebacher I, Dumfahrt H. Testing the Clinical Applicability of Resin Infiltration of Developmental Enamel Hypomineralization Lesions Using an In Vitro Model. Int J Clin Pediatr Dent 2019; 12 (2):126-132.

DOI: 10.5005/jp-journals-10005-1609

License: CC BY-NC 4.0

Published Online: 01-09-2019

Copyright Statement:  Copyright © 2019; The Author(s).


Aim: The aim of this study is to investigate the penetration abilities of a commercially available low-viscosity resin infiltrant into developmentally hypomineralized teeth in vitro. Materials and methods: Four extracted third molars of a 17-year-old patient with signs of developmental enamel hypomineralization (discoloration, increased opacity, and surface roughness) were infiltrated with a low-viscosity resin mixed with a fluorescent dye, according to the manufacturer's standard protocol. Four extracted molars with sound enamel or showing only initial fissure caries were used as a control group. Specimens were embedded in polymethylmethacrylate, and grindings were prepared. High-resolution projectional radiography of the grindings was performed, and, for one specimen, quantitative micro-computed tomography was used to measure hydroxyapatite density in enamel and dentin lesions. After decalcification, the grindings were examined by reflected bright-field microscopy, wide-field fluorescence microscopy, and confocal laser scanning microscopy. Fluorescence micrographs were superimposed on the radiographs and analyzed correlatively. Results: The pattern of hypo-/demineralization in enamel and dentin in developmentally hypomineralized teeth showed a good congruence with the pattern of resin infiltration. Cavitations and dentin tubules up to a depth of 2 mm beyond cavitations were filled by the infiltrant. In control teeth, the penetration of the infiltrant was limited to decalcified enamel areas (initial fissure caries). Conclusions: In vitro infiltration of developmentally hypomineralized enamel was successful. Clinical significance: Resin infiltration might be considered as a routine procedure in the treatment of developmentally hypomineralized teeth. Further investigations with higher sample sizes, different degrees of severity, different stages of lesion extension, and modified treatment protocols are necessary.

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