International Journal of Clinical Pediatric Dentistry

Register      Login

VOLUME 12 , ISSUE 1 ( January-February, 2019 ) > List of Articles

Original Article

Nanotechnology in Dentin Disinfection: Can We Preserve the Bond?

Prabhakar Attiguppe, Amrita P Tripathi, Suriyan Sugandhan, Saraswathi V Naik, Bikshavathi M Deepak

Keywords : Ag-Au (silver-gold) nanoparticles, Chlorhexidine, Microleakage, Resin tag

Citation Information : Attiguppe P, Tripathi AP, Sugandhan S, Naik SV, Deepak BM. Nanotechnology in Dentin Disinfection: Can We Preserve the Bond?. Int J Clin Pediatr Dent 2019; 12 (1):42-46.

DOI: 10.5005/jp-journals-10005-1590

License: CC BY-NC 4.0

Published Online: 01-02-2019

Copyright Statement:  Copyright © 2019; The Author(s).


Abstract

Aims: The aim of this study is to evaluate the effect of cavity disinfection with 2% chlorhexidine (CHX) and Ag-Au nanoparticles on microleakage and resin tag penetrability of composite restoration under in vitro conditions. Materials and methods: Twenty-five human permanent molars extracted for therapeutic reasons were used in the study. Class V cavity of standard dimension was prepared on the buccal and lingual surfaces of the teeth. The teeth were randomly allocated into two groups based on the cavity disinfectant used: group I being 2% CHX gluconate (chlorhexidina Friedrich and Bianca Mittelstadt (FGM)) and group II being cavity disinfectant containing Ag-Au nanoparticles (nanocare gold). In both the groups, the dentin was etched with 37% phosphoric acid and cavity disinfectants were applied following which dentine bonding agent and composite resin were applied and cured. The specimens were then viewed under a stereomicroscope and a scanning electron microscope for microleakage and resin tag formation, respectively. Results: The results were statistically analyzed using an independent “t” test. No significant difference was seen between the two groups with respect to both microleakage and resin tag penetration values (p > 0.05). Conclusion: The cavity disinfectant containing Ag-Au nanoparticles did not affect the sealing ability and resin tag penetrability of composite resin in permanent molars when compared with 2% CHX.


PDF Share
  1. Murdoch-Kinch CA, Mclean ME. Minimally invasive dentistry. J Am Dent Assoc 2003;134:87–95. DOI: 10.14219/jada.archive.2003.0021.
  2. Bocongel JS, Kraul AO, et al. Influence of disinfectant solutions on the tensile bond strength of a fourth generation dentin bonding agent. Pesq Odont Bras 2000;14(2):107–111. DOI: 10.1590/S1517-74912000000200003.
  3. Meiers JC, Kresin JC. Cavity disinfectants and dentin bonding. Oper Dent 1996 Jul–Aug;21(4):153–159.
  4. Carrilho MR, Carvalho RM, et al. Chlorhexidine preserves dentin bond in vitro. J Dent Res 2007 Jan;86(1):90–94. DOI: 10.1177/154405910708600115.
  5. Gürgan S, Bolay S, et al. Effect of disinfectant application methods on the bond strength of composite to dentin. J Oral Rehabil 1999 Oct;26(10):836–840. DOI: 10.1046/j.1365-2842.1999.00458.x.
  6. Singla M, Aggarwal V, et al. Effect of chlorhexidine cavity disinfection on microleakage in cavities restored with composite using a selfetching single bottle adhesive. J Conserv Dent 2011 Oct;14(4):374–377. DOI: 10.4103/0972-0707.87201.
  7. Lundström F, Krasse B. Streptococcus mutans and lactobacilli frequency in orthodontic patients; the effect of chlorhexidine treatments. Eur J Orthod 1987 May;9(2):109–116. DOI: 10.1093/ejo/9.2.109.
  8. Uzer Celik E, Tunac AT, et al. Antimicrobial activity of different disinfectants against cariogenic microorganisms. Braz Oral Res 2016 Nov 28;30(1):e125. DOI: 10.1590/1807-3107bor-2016.vol30.0125.
  9. Mackiewicz A, Kowalczyk DO. Microscopic evaluation of surface topography and chemical composition of nanocare gold. J Stoma 2014;67(6):826–840. DOI: 10.5604/00114553.1137107.
  10. Sharma V, Rampal P, et al. Shear bond strength of composite resin to dentin after application of cavity disinfectants – SEM study. Contemp Clin Dent 2011 Jul;2(3):155–159. DOI: 10.4103/0976-237X.86438.
  11. Stanislawczuk R, Amaral RC, et al. Chlorhexidine-containing acid conditioner preserves the longevity of resin–dentin bonds. Oper Dent 2009 Jul–Aug;34(4):481–490. DOI: 10.2341/08-016-L.
  12. Siso HS, Kustarci A, et al. Microleakage in resin composite restorations after antimicrobial pre-treatments: effect of KTP laser, chlorhexidine gluconate and Clearfil Protect Bond. Oper Dent 2009 May–Jun;34(3):321–327. DOI: 10.2341/08-96.
  13. Ceballos L, Osorio R, et al. Microleakage of composite restorations after acid or Er-YAG laser cavity treatments. Dent Mater 2001 Jul;17(4):340–346. DOI: 10.1016/S0109-5641(00)00092-0.
  14. Porenczuk A, Firlej P, et al. The laboratory comparison of shear bond strength and microscopic assessment of failure modes for a glassionomer cement and dentin bonding systems combined with silver nanoparticles. Acta Bioeng Biomech 2016;18(2):59–70.
  15. Lohbauer U, Wagner A, et al. Zirconia nanoparticles prepared by laser vaporization as fillers for dental adhesives. Acta Biomater 2010;6:4539–4546. DOI: 10.1016/j.actbio.2010.07.002.
  16. Fardal O, Turnbull RS. A review of the literature on use of chlorhexidine in dentistry. J Am Dent Assoc 1986;112(6):863–869.
  17. Perdigão J, Denehy GE, et al. Effects of chlorhexidine on dentin surfaces and shear bond strengths. Am J Dent 1994;7(2):81–84.
  18. Radovic I, Vulicevic ZR, et al. Morphological evaluation of 2- and 1-step self-etching system interfaces with dentin. Oper Dent 2006;31:710–718. DOI: 10.2341/05-145.
PDF Share
PDF Share

© Jaypee Brothers Medical Publishers (P) LTD.