Aims: The aim of the present study is to evaluate the effect of cavity disinfection with 2% chlorhexidine (CHX) and Ag-Au nanoparticles on microleakage and resin tag penetrability of composite restoration under in vitro conditions.
Materials and methods: Twenty-five human permanent molars extracted for therapeutic reasons were used in the study. Class V cavity of standard dimension was prepared on the buccal and lingual surfaces of the teeth. The teeth were randomly allocated into two groups based on the cavity disinfectant used; group I being 2% chlorhexidine gluconate (chlorhexidine FGM) and group II being cavity disinfectant containing Ag-Au nanoparticles (nanocare gold). In both the groups, the dentin was etched with 37% phosphoric acid and cavity disinfectants were applied following which dentine bonding agent and composite resin were applied and cured. The specimens were then viewed under stereomicroscope and scanning electron microscope for evaluation of microleakage and resin tag formation, respectively.
Results: The results were statistically analyzed using independent ‘t’ test. No significant difference was seen between the two groups with respect to the microleakage and resin tag penetration values (p > 0.05).
Conclusion: Cavity disinfectant containing Ag-Au nanoparticles had no effect on the sealing ability and resin tag penetrability of composite resin in permanent molars when compared with 2% CHX.
Clinical significance: Use of Cavity disinfectant containing Ag-Au nanoparticles with etch and rinse system can be preferred as it satisfies the ideal property of a cavity disinfectant which includes excellent antimicrobial action and non-detrimental effect on the sealing ability and resin tag penetrability.
Carrilho MR, Carvalho RM, de Goes MF, et al. Chlorhexidine preserves dentin bond in vitro. J Dent Res. 2007;86(1):90-94.
Gürgan S, Bolay S, Kiremitçi A. Effect of disinfectant application methods on the bond strength of composite to dentin. J Oral Rehabil. 1999;26(10):836-840.
Singla M, Aggarwal V, Kumar N. Effect of chlorhexidine cavity disinfection on microleakage in cavities restored with composite using a self-etching single bottle adhesive. J Conserv Dent. 2011; 14(4):374-377.
Lundström F, Krasse B. Streptococcus mutans and lactobacilli frequency in orthodontic patients; the effect of chlorhexidine treatments. Eur J Orthod. 1987;9(2):109-116.
Uzer Celik E, Tunac AT, Ates M, et al. Antimicrobial activity of different disinfectants against cariogenic microorganisms. Braz Oral Res. 2016;30(1):e125.
Mackiewicz A, Kowalczyk DO. Microscopic evaluation of surface topography and chemical composition of nanocare gold. J Stoma. 2014; 67(6):826-840.
Sharma V, Rampal P, Kumar S. Shear bond strength of composite resin to dentin after application of cavity disinfectants - SEM study. Contemp Clin Dent. 2011; 2(3):155-159.
Stanislawczuk R, Amaral RC, Zander-Grande C, et al. Chlorhexidine-containing acid conditioner preserves the longevity of resin-dentin bonds. Oper Dent. 2009;34(4):481-490.
Siso HS, Kustarci A, Göktolga EG. Microleakage in resin composite restorations after antimicrobial pre-treatments: effect of KTP laser, chlorhexidine gluconate and Clearfil Protect Bond. Oper Dent. 2009; 34(3):321-327.
Ceballos L, Osorio R, Toledano M, et al. Microleakage of composite restorations after acid or Er-YAG laser cavity treatments. Dent Mater. 2001; 17(4):340-346.
Porenczuk A, Firlej P, Szczepañska G, et al. The laboratory comparison of shear bond strength and microscopic assessment of failure modes for a glass-ionomer cement and dentin bonding systems combined with silver nanoparticles. Acta Bioeng Biomech. 2016; 18(2):59-70.
Lohbauer U, Wagner A, Belli R, Set al. Zirconia nanoparticles prepared by laser vaporization as fillers for dental adhesives. Acta Biomater. 2010;6(12):4539-4546.
Fardal O, Turnbull RS. A review of literature on use of chlorhexidine in dentistry. J. Am. Dent. Assoc. 1986; 112(6): 863-869.
Perdigao J, Denehy GE, Swift EJ Jr. Effects of chlorhexidine on dentin surfaces and shear bond strengths. Am J Dent. 1994;7(2):81-84
Radovic I, Vulicevic ZR, Garcia-Godoy F. Morphological evaluation of 2- and 1-step self-etching system interfaces with dentin. Oper Dent. 2006;31:710-718