Evaluation of Chemokines in the Gingival Crevicular Fluid of Children with Down Syndrome
Harshini Togaru, Veerakishore Kumar Reddy, Naveen K Kommineni, Prathyusha Padakandla, John P Indupalli, Swapna P Nanga
Chemokines, Down syndrome, Gingival crevicular fluid, Inflammation, Macrophage inflammatory protein 1&alpha , Macrophage inflammatory protein 1â
Citation Information :
Togaru H, Reddy VK, Kommineni NK, Padakandla P, Indupalli JP, Nanga SP. Evaluation of Chemokines in the Gingival Crevicular Fluid of Children with Down Syndrome. Int J Clin Pediatr Dent 2018; 11 (4):288-293.
Aim: The goal of the study was to detect the presence of macrophage inflammatory protein (MIP)-1α and MIP-1β and to estimate their levels in gingival crevicular fluid (GCF) of children with Down syndrome.
Materials and methods: MIP-1α and MIP-1β levels were estimated in GCF samples of 20 healthy and 20 Down syndrome individuals. Gingival status was assessed by measuring the gingival index (GI), plaque index (PI), clinical attachment level (CAL), and probing pocket depth (PPD).
The GCF samples were obtained from the subjects and MIP-1α and MIP-1β levels were quantified by enzyme-linked immunosorbent assay (ELISA).
Results: The mean MIP-1α concentrations in healthy and Down syndrome individuals were 209 and 1411 pg/μL respectively, and MIP-1α levels were 342 and 1404 pg/μL respectively.
The levels of MIP-1α and MIP-1β in the GCF of subjects with Down syndrome were significantly higher than in the healthy individual, and statistically significant differences were present among the two groups.
Conclusion: The GCF showed dynamic changes according to the severity of periodontal disease, and the levels of MIP-1α and MIP-1β had a strong relationship with clinical parameters. The MIP-1α and MIP-1β can therefore be considered as novel biomarkers in the biological mechanism underlying the pathogenesis of periodontal disease.
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