Citation Information :
Hugar S, Patel PM, Nagmoti J, Uppin C, Mistry L, Dhariwal N. An in vitro Comparative Evaluation of Efficacy of Disinfecting Ability of Garlic Oil, Neem Oil, Clove Oil, and Tulsi Oil with autoclaving on Endodontic K Files tested against Enterococcus faecalis. Int J Clin Pediatr Dent 2017; 10 (3):283-288.
Aim: To comparatively evaluate the efficacy of disinfecting ability of garlic oil, neem oil, clove oil, and tulsi oil with autoclaving on endodontic K files tested against Enterococcus faecalis.
Materials and methods: Fifty endodontic K files were exposed to the test micro-organism and checked for its disinfecting ability using three different methods.
Result: Garlic oil, clove oil, tulsi oil and autoclave showed considerable effectiveness against E. faecalis except neem oil.
Conclusion: Garlic oil, clove oil and tulsi oil are an effective disinfectant and can be used as an alternative to autoclaving against the test micro-organism.
Clinical Significance: Herbs and herbal extracts are a natural and harmless way of controlling infection. These products are readily available and comparable to gold standard, thus can have its applications in rural India.
Al-Jamell D, Al-Nasrawi S, Al Quraine N, Aljdaimi, A. The effectiveness of three different methods for sterilization of the endodontic files (an in vitro study). Adv Lif Sci Technol 2014;27:1-5.
Venkatasubramanian R, Jayanthi, Das UM, Bhatnagar S. Comparison of the effectiveness of sterilizing endodontic files by 4 different methods: an in vitro study. J Indian Soc Pedod Prev Dent 2010 Jan-Mar;28(1):2-5.
Aslam A, Panuganti V, Nanjundasetty JK, Halappa M, Krishna VH. Knowledge and attitude of endodontic postgraduate students toward sterilization of endodontic files: a cross-sectional study. Saudi Endod J 2014 Jan;4(1):18.
Roth TP, Whitney SI, Walker SG, Friedman S. Microbial contamination of endodontic files received from the manufacturer. J Endod 2006 Jul;32(7):649-651.
Aasim SA, Mellor AC, Qualtrough AJ. The effect of presoaking and time in the ultrasonic cleaner on the cleanliness of sterilized endodontic files. Int Endod J 2006 Feb;39(2):143-149.
Neal RG, Craig RG, Powers JM. Effect of sterilization and irrigants on the cutting ability of stainless steel files. J Endod 1983 Mar;9(3):93-96.
Estrela C, Silva JA, de Alancer AHG, Leles CR, Decurcio DA. Efficacy of sodium hypochlorite and chlorhexidine against Enterococcus faecalis—a systematic review. J Appl Oral Sci 2008 Nov-Dec;16(6):364-368.
Ambareen Z, Chinappa A. Go green-keep the root canal clean!!!. Int J Dent Sci Res 2014 Dec;2(6B):21-25.
Stuart CH, Schwatrz SA, Beeson TJ, Owatz CB. Enterococcus faecalis: its Role in Root Canal Treatment Failure and Current Concepts in Retreatment. J Endod 2006 Feb;32(2):93-98.
Groppo FC, Ramacciato JC, Simões RP, Flório FM, Sartoratto A. Antimicrobial activity of garlic, tea tree oil, and chlorhexidine against oral microorganisms. Int Dent J 2002 Dec;52(6): 433-437.
Tsao S, Yin M. In vitro activity of garlic oil and four diallyl sulphides against antibiotic-resistant Pseudomonas aeruginosa and Klebsiella pneumoniae. J Antimicrob Chemother 2001 May;47(5):665-670.
Bakri IM, Douglos CWI. Inhibitory effect of garlic extract on oral bacteria. Arch Oral Biol 2005 Aug;50(7):645-651.
Ruddock PS, Liao M, Foster BC, Lawson L, Arnason JT, Dillon JA. Garlic natural health products exhibit variable constituent levels and antimicrobial activity against Neisseria gonorrhoeae, Staphylococcus aureus and Enterococcus faecalis. Phytother Res 2005 Apr;19(4):327-334.
Ankri, S, Mirelman D. Antimicrobial properties of allicin from garlic. Microbes Infect 1999 Feb;1(2):125-129.
Nascimento GGF, Locatelli J, Freitas PC, Silva GL. Antibacterial activity of plant extracts and phytochemicals on antibiotic- resistant bacteria. Braz J Microbiol 2000 Oct-Dec;31(4): 247-256.
Joseph B, Sujatha S. Bioactive compounds and its autochthonous microbial activities of the extract and clove oil (Syzygiumaromaticum L.) on some food borne pathogens. Asian J Biol Life Sci 2011 Jan;4(1):35-43.
Hugar SM, Mistry LN, Hogade S, Badkar CM. An in vitro comparative evaluation of efficacy of disin-fecting ability of garlic oil (lasuna), clove leaf oil (la-vang) and autoclaving on endodontic k files tested against Enterococcus faecalis. Int Ayu Med J 2015 Aug;3(8):2277-2284.
Bayoub K, Baibai T, Mountassif D, Retmane A, Soukri A. Antibacterial activities of the crude ethanol extracts of medicinal plants against Listeria monocytogenes and some other pathogenic strains. Afr J Biotechnol 2010 Jul;9(27):4251-4258.
Fine DH, Furgang D, Barnett ML, Drew C, Steinberg L, Charles CH, Vincent JW. Effect of an essential oil containing antiseptic mouthrinse on plaque and salivary Streptococcus mutans levels. J Clin Periodontol 2000 Mar;27(3):157-161.
Agarwal P, Nagesh L. Comparative evaluation of efficacy of 0.2% Chlorhexidine, Listerine and Tulsi extract mouth rinses on salivary Streptococcus mutans count of high school children–RCT. Contemp Clin Trials 2011 Nov;32(6):802-808.
Gupta A, Duhan J, Tewari S, Sangwan P, Yadav A, Singh G, Juneja R, Saini H. Comparative evaluation of antimicrobial efficacy of Syzygiumaromaticum, Ocimum sanctum and Cinnamomum zeylanicum plant extracts against Enterococcus faecalis: a preliminary study. Int Endod J 2013 Aug;46(8):775-783.
Subbiya A, Mahalakshmi K, Pushpangadan S, Padmavathy K, Vivekanandan P, Sukumaran VG. Antibacterial efficacy of Mangiferaindical. kernel and Ocimum sanctum L. leaves against Enterococcus faecalis dentinal biofilm. J Conserv Dent 2013 Sep;16(5):454-457.
Elavarasu S, Abinaya P, Elanchezhiyan S, Thangakumaran, Venilla K, Naziya KB. Evaluation of anti-plaque microbial activity of Azadirachtaindica (neem oil) in vitro: a pilot study. J Pharm Bioallied Sci 2012 Aug;4(Suppl 2):S394-S396.
Bohora A, Hegde V, Kokate S. Comparison of the antibacterial efficiency of neem leaf extract and 2% sodium hypochlorite against E faecalis, C albicans and mixed culture—an in vitro study. Endodontology 2010;22(1): 10-14.
Kumar D, Sidhu P. The antimicrobial activity of Azardirachta indica, Glycyrrhiza glabra, Cinnamum zeylanicum, Syzygium aromaticum, Accacia nilotica on Streptococcus mutans and Enterococcus faecalis—an in vitro study. Edit Orig Res 2011;23(1):18-25.
Farooqui NA, Dey A, Singh GN, Easwari TS. Antibacterial potential of Nimbolide from Azadirachtaindica. Int J Pharm Pharm Sci 2014 May;6(5):636-638.
Oyawale FA, Olaoye AE. Design and construction of an autoclave. Pac J Sci Tech 2007 Jan;8(2):224-230.