International Journal of Clinical Pediatric Dentistry

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VOLUME 10 , ISSUE 1 ( January-March, 2017 ) > List of Articles

Original Article

Effects of Fluoride Varnish on Streptococcus mutans Count in Saliva

Sourabh Badjatia, Rini G Badjatia, K Thanveer, Ajith CG Krishnan

Keywords : Dental caries, Fluoride varnish, Streptococcus mutans

Citation Information : Badjatia S, Badjatia RG, Thanveer K, Krishnan AC. Effects of Fluoride Varnish on Streptococcus mutans Count in Saliva. Int J Clin Pediatr Dent 2017; 10 (1):62-66.

DOI: 10.5005/jp-journals-10005-1409

License: CC BY-NC 4.0

Published Online: 01-04-2009

Copyright Statement:  Copyright © 2017; The Author(s).


Aim: To evaluate the effect of fluoride varnish on Streptococcus mutans count in saliva among 12-year-old school children. Materials and methods: A field experiment was conducted to evaluate the effects of fluoride varnish on S. mutans count in saliva among 12-year-old school children. A total of 42 school-going children attending schools in Vadodara district, Gujarat, India, were divided into two groups. Group I was treated with fluoride varnish and group II received no treatment. Assessment of S. mutans was carried out at baseline and 3 to 6 months postfluoride varnish application. Friedman analysis of variance test and post hoc test were applied to detect statistically significant differences between baseline, 3 to 6 months of fluoride varnish application, and also between groups I and II. Results: The mean number of salivary S. mutans value found in case group at baseline, 3 to 6 months was 31.23 ± 1.119, 9.27 ± 0.852, and 9.39 ± 0.908 × 104 colony-forming unit CFU/mL respectively. The difference in S. mutans count from baseline to 3 to 6 months was highly statistically significant (p = 0.000), but the difference from 3 to 6 months was not statistically significant (p = 0.142). In control group, the mean S. mutans value found at baseline, 3 to 6 months was 30.63 ± 1.436, 31.23 ± 1.351, and 31.40 ± 1.374 × 104 CFU/mL respectively. The differences between these values were not statistically significant (p = 0.11). Conclusion: Statistically significant reduction in S. mutans count in saliva was seen 3 to 6 months after fluoride varnish application.

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  1. International Obfuscated C Code Contest Report on Dental Caries [Internet]. 1995 [cited 2012 May 3]. Available from:
  2. Van Houte J. Role of micro-organisms in caries etiology. J Dent Res 1994 Mar:73(3):672-681.
  3. Loesche WJ. Role of streptococcus mutans in human dental decay. Microbiol Rev 1986 Dec;50(4):353-380.
  4. Bratthall, D.; Carlsson, P. Clinical microbiology of human saliva: chemistry and microbiology. Boca Raton: CRC Press;
  5. p. 204-223.
  6. ten Cate JM. Current concepts on the theories of the mechanism of action of fluoride. Acta Odontol Scand 1999 Dec;57(6);325-329.
  7. Marinho, VCC.; Higgins, JPT.; Logan, S.; et al. Fluoride varnishes for preventing dental caries in children and adolescents. Cochrane Database of Systematic Reviews 2013, Issue 7. Available from: 14651858.CD002279.pub2/abstract.
  8. Peter, S. Essentials of preventive and community dentistry. 1st ed. New Delhi: Arya (Medi) Publishing House: New Delhi 2002.
  9. Hamilton IR. Biochemical effects of fluoride on oral bacteria. J Dent Res 1990 Feb;69 Spec No:660-667.
  10. Association of State and Territorial Dental Directors – Fluorides Committee. Fluoride varnish: an evidence-based approach: research brief [Internet].2007 Sep. [cited 2012 Jun 3]. Available from:
  11. Nyvad B. Diagnosis versus detection of caries. Caries Res 2004 May-Jun;38(3):192-198.
  12. Shi S, Liang Q, Hayashi Y, Yakushiji M, Machida Y. The relationship between caries activity and the status of dental caries – application of the dentocult SM method. Chin J Dent Res 1998 Dec;1(3):52-55.
  13. Murray, JJ.; Rugg-Gunn, AJ.; Jenkins, GN. Fluorides in caries prevention. 3rd ed. Mumbai: Varghese Publishing House.
  14. Fluoride varnish – an evidence-based approach research brief association of state and territorial dental directors fluorides committee September 2007. Available from: http://www.
  15. Zickert I, Emilson CG. Effect of a fluoride-containing varnish on streptococcus mutans in plaque and saliva. Scand J Dent Res 1982 Dec;90(6):423-428.
  16. Araujo AM, Naspitz GM, Chelotti A, Cai S. Effect of cervitec on mutans streptococci in plaque and on caries formation on occlusal fissures of erupting permanent molars. Caries Res 2002 Sep-Oct;36(5):373-376.
  17. Ekenbäck SB, Linder LE, Lönnies H. Effect of four dental varnishes on the colonization of cariogenic bacteria on exposed sound root surfaces. Caries Res 2000 Jan-Feb;34(1):70-74.
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