Comparison of Antimicrobial Efficacy of Diode Laser, Triphala, and Sodium Hypochlorite in Primary Root Canals: A Randomized Controlled Trial
Seby Thomas, Baby John, Geetha Priya, S Kumar
Laser, Sodium hypochlorite, Triphala
Citation Information :
Thomas S, John B, Priya G, Kumar S. Comparison of Antimicrobial Efficacy of Diode Laser, Triphala, and Sodium Hypochlorite in Primary Root Canals: A Randomized Controlled Trial. Int J Clin Pediatr Dent 2017; 10 (1):14-17.
Aim: To evaluate the antimicrobial efficacy of diode laser, triphala, and sodium hypochlorite (NaOCl) against Enterococc-cus faecalis contaminated primary root canals.
Materials and methods: Forty-nine single-rooted human primary teeth were reduced up to cemento-enamel junction and biomechanically prepared. After sterilization, five teeth were selected as negative controls and remaining teeth were inoculated with E. faecalis. The teeth were then randomly divided into four groups. The first group was irradiated with diode laser, the second group was irrigated with sodium hypochlorite, and the third group with triphala solution. The fourth group served as the positive control. The antimicrobial efficacy was tested by collecting transfer fluid saline from the canals and counting the colony forming units (CFUs) of viable E. faecalis on agar plates. The Mann-Whitney test was used to analyze the results, using Statistical Package for the Social Sciences software version 19.
Results: The results showed that mean bacterial CFU were 8.00 ± 7.87 for laser, 58.60 ± 16.63 for triphala, and 69.80 ± 19.57 for NaOCl. Laser group showed significant reduction in the colony count compared to the other groups. Triphala group showed better antibacterial activity than NaOCl, but the difference was not statistically significant.
Conclusion: Laser was most effective against E. faecalis and triphala can be used as an alternative disinfectant to NaOCl in primary root canals.
Murvindran V, Raj D J. Antibiotics as an Intracanal Medicament in Endodontics. J Pharm Sci Res 2014;6(9):297-301.
Rana V, Baba SM, Pandey A. Bacteriology of infected deciduous root canal – a review. People J Sci Res 2009;2(2):45-48.
Estrela C, Silva JA, de Alencar AH, Leles CR, Decurcio DA. Efficacy of sodium hypochlorite and chlorhexidine against Enterococcus faecalis – a systematic review. J Appl Oral Sci 2008 Nov-Dec;16(6):364-368.
Gutknecht N, van Gogswaardt D, Conrads G, Apel C, Schubert C, Lampert F. Diode laser radiation and its bactericidal effect in root canal wall dentin. J Clin Laser Med Surg 2000 Apr;18(2):57-60.
Moritz A, Gutknecht N, Schoop U, Goharkhay K, Doertbudak O, Sperr W. Irradiation of infected root canals with a diode laser in vivo: results of microbiological examinations. Lasers Surg Med 1997;21(3):221-226.
Prabhakar J, Senthilkumar M, Priya MS, Mahalakshmi K, Sehgal PK, Sukumaran VG. Evaluation of antimicrobial efficacy of herbal alternatives (Triphala and green tea polyphenols), MTAD, and 5% sodium hypochlorite against Enterococcus faecalis biofilm formed on tooth substrate: an in vitro study. J Endod 2010 Jan;36(1):83-86.
Mehrvarzfar P, Saghiri MA, Asatourian A, Fekrazad R, Karamifar K, Eslami G, Dadresanfar B. Additive effect of a diode laser on the antibacterial activity of 2.5% NaOCl, 2% CHX and MTAD against Enterococcus faecalis contaminating root canals: an in vitro study. J Oral Sci 2011 Sep;53(3): 355-360.
Stuart CH, Schwartz SA, Beeson TJ, Owatz CB. Enterococcus faecalis: its role in root canal treatment failure and current concepts in retreatment. J Endod 2006 Feb;32(2):93-98.
Siqueira JF Jr, Rôças IN, Favieri A, Lima KC. Chemomechanical reduction of the bacterial population in the root canal after instrumentation and irrigation with 1%, 2.5%, and 5.25% sodium hypochlorite. J Endod 2000 Jun;26(6):331-335.
George S, Kishen A, Song KP. The role of environmental changes on monospecies biofilm formation on root canal wall by Enterococcus faecalis. J Endod 2005 Dec;31(12):867-872.
Moritz, A.; Beer, F.; Goharkhay, K.; Schoop, U. Laser supported root canal sterilization. Oral Laser Application. 1st ed. Chicago (IL): Quintessence Publishing; 2006. p. 254-277.
de Souza EB, Cai S, Simionato MR, Lage-Marques JL. Highpower diode laser in the disinfection in depth of the root canal dentin. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2008 Jul;106(1):e68-e72.
Schoop U, Kluger W, Moritz A, Nedjelik N, Georgopoulos A, Sperr W. Bactericidal effect of different laser systems in the deep layers of dentin. Lasers Surg Med 2004;35(2):111-116.
Kuvvetli SS, Sandalli N, Topcuoglu N, Kulekci G. Antibacterial efficacy of diode and Er:YAG laser irradiation in experimentally contaminated primary molar root canals. J Clin Pediatr Dent 2009 Fall;34(1):43-48.
Parker S. Laser regulation and safety in general dental practice. Br Dent J 2007 May;202(9):523-532.
Nara A, Dhanu, Chandra A, Anandakrishna L, Dhananjaya. Comparative Evaluation of Antimicrobial Efficacy of MTAD, 3% NaOCl and Propolis Against E Faecalis. Int J Clin Pediatr Dent 2010 Jan-Apr;3(1):21-25.
de Rezende GP, da Costa LR, Pimenta FC, Baroni DA. In vitro antimicrobial activity of endodontic pastes with propolis extracts and calcium hydroxide: a preliminary study. Braz Dent J 2008;19(4):301-305.