International Journal of Clinical Pediatric Dentistry

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VOLUME 10 , ISSUE 1 ( January-March, 2017 ) > List of Articles

Original Article

Salivary Proteins—A Barrier on Enamel Demineralization: An in vitro Study

Ankit R Sajnani

Keywords : Albumin, Demineralization, Enamel, Salivary protein

Citation Information : Sajnani AR. Salivary Proteins—A Barrier on Enamel Demineralization: An in vitro Study. Int J Clin Pediatr Dent 2017; 10 (1):10-13.

DOI: 10.5005/jp-journals-10005-1398

License: CC BY-NC 4.0

Published Online: 01-12-2008

Copyright Statement:  Copyright © 2017; The Author(s).


Aim: The aim of this study is to evaluate the protective effect of the salivary proteins on the demineralization of enamel. Materials and methods: Twenty freshly extracted human molar teeth were used in this study. Enamel samples (2 mm thickness) were prepared from the buccal and lingual surfaces of the teeth selected. An acid-resistant nail varnish was used to cover every aspect of the sample, except an area of 5 × 5 mm limited by an adhesive tape. After drying, the adhesive tape was removed, exhibiting a rectangular area on the enamel surface. Samples were divided into two groups:   • Group I (10 samples): Each sample was coated by 100 μg of albumin for 2 hours at 37°C.   • Group II (10 samples): Each sample was exposed to 100 μL of deionized water. Samples were washed by dipping once in deionized water. They were then disposed into individual tubes containing demineralization solution for 1, 2, 3, and 4 minutes at 37°C with gentle agitation. The demineralization solution was utilized to determine the calcium loss from specimens at 1, 2, 3, 4 minutes using an ultraviolet-visible spectrophotometer. Results: Calcium loss was less from the albumin-coated samples than control group at all times and was statistically significant (p < 0.05). Also, calcium loss was maximum at the end of 1 minute, and it decreased as time interval increased and was statistically significant (p < 0.001). Conclusion: Albumin has provided a strong protection against enamel demineralization at all times compared to the one without it.

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